Subnanometre single-molecule localization, registration and distance measurements

Nature

Volumn 466, Issue 7306, 2010, Pages 647-651

  Pertsinidis, Alexandros ^a,b   Zhang, Yunxiang ^a,b   Chu, Steven ^a,b,c,d,e  

^a STANFORD UNIVERSITY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

^e U S DEPARTMENT OF ENERGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIFFRACTION; FLUORESCENCE; IMAGE RESOLUTION; IMAGING METHOD; MICROSCOPY; OPTICAL INSTRUMENT; OPTICAL METHOD; WAVELENGTH;

ACCURACY; ARTICLE; DISTANCE PERCEPTION; FLUORESCENCE ANALYSIS; MICROWAVE RADIATION; OPTICAL RESOLUTION; OPTICS; PHOTON; PRIORITY JOURNAL; SEPARATION TECHNIQUE; SIGNAL NOISE RATIO; SPECTROSCOPY; CALIBRATION; CHEMISTRY; COLOR; DEVICES; FLUORESCENCE; MICROSCOPY; NANOTECHNOLOGY; PROCEDURES; SENSITIVITY AND SPECIFICITY; UNCERTAINTY;

BUFFERS; CADHERINS; CALIBRATION; COLOR; DNA; FLUORESCENCE; FLUORESCENT DYES; MICROSCOPY; MICROSPHERES; NANOTECHNOLOGY; PHOTONS; SENSITIVITY AND SPECIFICITY; SILICON; UNCERTAINTY;

BUFFER; CADHERIN; DNA; FLUORESCENT DYE; MICROSPHERE; SILICON;

EID: 77955175217     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature09163     Document Type: Article

References (30)

1. Bobroff, N. Position measurement with a resolution and noise-limited instrument. Rev. Sci. Instrum. 57, 1152-1157 (1986).
2. Yildiz, A. et al. Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization. Science 300, 2061-2065 (2003).
3. Gordon, M. P., Ha, T. & Selvin, P. R. Single-molecule high-resolution imaging with photobleaching. Proc. Natl Acad. Sci. USA 101, 6462-6465 (2004).
4. Qu, X. et al. Nanometer-localized multiple single-molecule fluorescence microscopy. Proc. Natl Acad. Sci. USA 101, 11298-11303 (2004).
5. Ram, S., Ward, E. S. & Ober, R. J. Beyond Rayleigh's criterion: a resolution measure with application to single-molecule microscopy. Proc. Natl Acad. Sci. USA 103, 4457-4462 (2006).
6. Betzig, E. et al. Imaging intracellular fluorescent proteins at nanometer resolution. Science 313, 1642-1645 (2006).
7. Rust, M. J., Bates, M. & Zhuang, X. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nature Methods 3, 793-796 (2006).
8. Hess, S. T., Girirajan, T. P. K. & Mason, M. D. Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. Biophys. J. 91, 4258-4272 (2006).
9. Burns, D. H. et al. Strategies for attaining superresolution using spectroscopic data as constraints. Appl. Opt. 24, 154-161 (1985).
10. Bornfleth, H. et al. High-precision distance measurements and volume-conserving segmentation of objects near and below the resolution limit in three-dimensional confocal fluorescence microscopy. J. Microsc. 189, 118-136 (1998).
11. Betzig, E. Proposed method for molecular optical imaging. Opt. Lett. 20, 237-239 (1995).
12. van Oijen, A. M. et al. Far-field fluorescence microscopy beyond the diffraction limit. J. Opt. Soc. Am. A 16, 909-915 (1999).
13. Lacoste, T. D. et al. Ultrahigh-resolution multicolor colocalization of single fluorescent probes. Proc. Natl Acad. Sci. USA 97, 9461-9466 (2000).
14. Antelman, J. et al. Nanometer distance measurements between multicolor quantum dots. Nano Lett. 9, 2199-2205 (2009).
15. Koyama-Honda, I. et al. Fluorescence imaging for monitoring the colocalization of two single molecules in living cells. Biophys. J. 88, 2126-2136 (2005).
16. Churchman, L. S. et al. Single molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time. Proc. Natl Acad. Sci. USA 102, 1419-1423 (2005).
17. Heilemann, M. et al. High-resolution colocalization of single dye molecules by fluorescence lifetime imaging microscopy. Anal. Chem. 74, 3511-3517 (2002).
18. Hell, S. W. & Wichmann, J. Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Opt. Lett. 19, 780-782 (1994).
19. Klar, T. A. et al. Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission. Proc. Natl Acad. Sci. USA 97, 8206-8210 (2000).
20. Rittweger, E. et al. STED microscopy reveals crystal colour centres with nanometric resolution. Nature Photon. 3, 144-147 (2009).
21. Lauer, T. R. The photometry of undersampled point-spread functions. Publ. Astron. Soc. Pacif. 111, 1434-1443 (1999).
22. Boggon, T. J. et al. C-cadherin ectodomain structure and implications for cell adhesion mechanisms. Science 296, 1308-1313 (2002).
23. 21 dependence and mutational analysis reveal molecular details of E-cadherin homoassociation. EMBO J. 18, 1738-1747 (1999).
24. He, W., Cowin, P. & Stokes, D. L. Untangling desmosomal knots with electron tomography. Science 302, 109-113 (2003).
25. Chappuis-Flament, S. et al. Multiple cadherin extracellular repeats mediate homophilic binding and adhesion. J. Cell Biol. 154, 231-243 (2001).
26. Perret, E. et al. Trans-bonded pairs of E-cadherin exhibit a remarkable hierarchy of mechanical strengths. Proc. Natl Acad. Sci. USA 101, 16472-16477 (2004).
27. Zhang, Y. et al. Resolving cadherin interactions and binding cooperativity at the single-molecule level. Proc. Natl Acad. Sci. USA 106, 109-114 (2009).
28. Pokutta, S. et al. Conformational changes of the recombinant extracellular domain of E-cadherin upon calcium binding. Eur. J. Biochem. 223, 1019-1026 (1994).
29. Koch, A. W. et al. Calcium binding and homoassociation of E-cadherin domains. Biochemistry 36, 7697-7705 (1997).
30. Nagar, B. et al. Structural basis of calcium-induced E-cadherin rigidification and dimerization. Nature 380, 360-364 (1996).