High-precision distance measurements and volume-conserving segmentation of objects near and below the resolution limit in three-dimensional confocal fluorescence microscopy

Journal of Microscopy

Volumn 189, Issue 2, 1998, Pages 118-136

  Bornfleth, H ^a   Satzler K ^a,b   Eils, R ^a   Cremer, C ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b MAX PLANCK INSTITUTE FOR MEDICAL RESEARCH   (Germany)

Author keywords

3D segmentation; Image analysis; Multiple labelling; Quantitative confocal laser scanning microscopy; Signal to noise ratio; Virtual microscopy

Indexed keywords

DISTANCE MEASUREMENT; FLUORESCENCE; FLUORESCENCE MICROSCOPY; IMAGE SEGMENTATION; OPTICAL TRANSFER FUNCTION; VOLUME MEASUREMENT;

3D SEGMENTATION; CONFOCAL LASER SCANNING MICROSCOPY; CONFOCAL-LASER-SCANNING-MICROSCOPY; HIGH-PRECISION; IMAGE-ANALYSIS; LABELINGS; MULTIPLE LABELING; POINT-SPREAD FUNCTION; QUANTITATIVE CONFOCAL LASER SCANNING MICROSCOPY; VIRTUAL MICROSCOPY;

SIGNAL TO NOISE RATIO;

ACCURACY; ALGORITHM; ARTICLE; CALIBRATION; CONFOCAL MICROSCOPY; FLUORESCENCE MICROSCOPY; PRIORITY JOURNAL; SIGNAL NOISE RATIO; THREE DIMENSIONAL IMAGING;

EID: 0031886237     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.1998.00276.x     Document Type: Article

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