-
2
-
-
34250872269
-
Minimalist active-site redesign: teaching old enzymes new tricks
-
Toscano M.D., Woycechowsky K.J., Hilvert D. Minimalist active-site redesign: teaching old enzymes new tricks. Angew. Chem. Int. Ed. 2007, 46:3212-3236.
-
(2007)
Angew. Chem. Int. Ed.
, vol.46
, pp. 3212-3236
-
-
Toscano, M.D.1
Woycechowsky, K.J.2
Hilvert, D.3
-
3
-
-
0035843170
-
Industrial biocatalysis today and tomorrow
-
Schmid A., Dordick J.S., Hauer B., Kiener A., Wubbolts M., Witholt B. Industrial biocatalysis today and tomorrow. Nature 2001, 409:258-268.
-
(2001)
Nature
, vol.409
, pp. 258-268
-
-
Schmid, A.1
Dordick, J.S.2
Hauer, B.3
Kiener, A.4
Wubbolts, M.5
Witholt, B.6
-
4
-
-
36148958632
-
Engineered alkane-hydroxylating cytochrome P450(BM3) exhibiting nativelike catalytic properties
-
Fasan R., Chen M.M., Crook N.C., Arnold F.H. Engineered alkane-hydroxylating cytochrome P450(BM3) exhibiting nativelike catalytic properties. Angew. Chem. Int. Ed. 2007, 46:8414-8418.
-
(2007)
Angew. Chem. Int. Ed.
, vol.46
, pp. 8414-8418
-
-
Fasan, R.1
Chen, M.M.2
Crook, N.C.3
Arnold, F.H.4
-
5
-
-
34047128801
-
Stability of biocatalysts
-
Polizzi K.M., Bommarius A.S., Broering J.M., Chaparro-Riggers J.F. Stability of biocatalysts. Curr. Opin. Chem. Biol. 2007, 11:220-225.
-
(2007)
Curr. Opin. Chem. Biol.
, vol.11
, pp. 220-225
-
-
Polizzi, K.M.1
Bommarius, A.S.2
Broering, J.M.3
Chaparro-Riggers, J.F.4
-
6
-
-
0035424955
-
Engineering proteins for thermostability: the use of sequence alignments versus rational design and directed evolution
-
Lehmann M., Wyss M. Engineering proteins for thermostability: the use of sequence alignments versus rational design and directed evolution. Curr. Opin. Biotechnol. 2001, 12:371-375.
-
(2001)
Curr. Opin. Biotechnol.
, vol.12
, pp. 371-375
-
-
Lehmann, M.1
Wyss, M.2
-
7
-
-
0028111743
-
Sequence statistics reliably predict stabilizing mutations in a protein domain
-
Steipe B., Schiller B., Plückthun A., Steinbacher S. Sequence statistics reliably predict stabilizing mutations in a protein domain. J. Mol. Biol. 1994, 240:188-192.
-
(1994)
J. Mol. Biol.
, vol.240
, pp. 188-192
-
-
Steipe, B.1
Schiller, B.2
Plückthun, A.3
Steinbacher, S.4
-
8
-
-
0033779402
-
Temperature adaptation of enzymes: lessons from laboratory evolution
-
Wintrode P.L., Arnold F.H. Temperature adaptation of enzymes: lessons from laboratory evolution. Adv. Protein Chem. 2001, 55:161-225.
-
(2001)
Adv. Protein Chem.
, vol.55
, pp. 161-225
-
-
Wintrode, P.L.1
Arnold, F.H.2
-
9
-
-
0031022599
-
β-Turn propensities as paradigm for the analysis of structural motifs to engineer protein stability
-
Ohage E.C., Graml W., Walter M.M., Steinbacher S., Steipe B. β-Turn propensities as paradigm for the analysis of structural motifs to engineer protein stability. Protein Sci. 1997, 6:233-241.
-
(1997)
Protein Sci.
, vol.6
, pp. 233-241
-
-
Ohage, E.C.1
Graml, W.2
Walter, M.M.3
Steinbacher, S.4
Steipe, B.5
-
10
-
-
0033963277
-
From DNA sequences to improved functionality: using protein sequence comparisons to rapidly design a thermostable consensus phytase
-
Lehmann M., Kostrewa D., Wyss M., Brugger R., D'Arcy A., Pasamontes L., van Loon A.P.G.M. From DNA sequences to improved functionality: using protein sequence comparisons to rapidly design a thermostable consensus phytase. Protein Eng. 2000, 13:49-57.
-
(2000)
Protein Eng.
, vol.13
, pp. 49-57
-
-
Lehmann, M.1
Kostrewa, D.2
Wyss, M.3
Brugger, R.4
D'Arcy, A.5
Pasamontes, L.6
van Loon, A.P.G.M.7
-
11
-
-
18744380008
-
Consensus-derived structural determinants of the ankyrin repeat motif
-
Mosavi L.K., Minor D.L., Peng Z.Y. Consensus-derived structural determinants of the ankyrin repeat motif. Proc. Natl Acad. Sci. USA 2002, 99:16029-16034.
-
(2002)
Proc. Natl Acad. Sci. USA
, vol.99
, pp. 16029-16034
-
-
Mosavi, L.K.1
Minor, D.L.2
Peng, Z.Y.3
-
12
-
-
0037648552
-
Design of stable α-helical arrays from an idealized TPR motif
-
Main E.R.G., Xiong Y., Cocco M.J., D'Andrea L., Regan L. Design of stable α-helical arrays from an idealized TPR motif. Structure 2003, 11:497-508.
-
(2003)
Structure
, vol.11
, pp. 497-508
-
-
Main, E.R.G.1
Xiong, Y.2
Cocco, M.J.3
D'Andrea, L.4
Regan, L.5
-
13
-
-
0042780350
-
Designing repeat proteins: well-expressed, soluble and stable proteins from combinatorial libraries of consensus ankyrin repeat proteins
-
Binz H.K., Stumpp M.T., Forrer P., Amstutz P., Plückthun A. Designing repeat proteins: well-expressed, soluble and stable proteins from combinatorial libraries of consensus ankyrin repeat proteins. J. Mol. Biol. 2003, 332:489-503.
-
(2003)
J. Mol. Biol.
, vol.332
, pp. 489-503
-
-
Binz, H.K.1
Stumpp, M.T.2
Forrer, P.3
Amstutz, P.4
Plückthun, A.5
-
14
-
-
38849157945
-
Development of a thermostable glucose dehydrogenase by a structure-guided consensus concept
-
Vazquez-Figueroa E., Chaparro-Riggers J., Bommarius A.S. Development of a thermostable glucose dehydrogenase by a structure-guided consensus concept. ChemBioChem 2007, 8:2295-2301.
-
(2007)
ChemBioChem
, vol.8
, pp. 2295-2301
-
-
Vazquez-Figueroa, E.1
Chaparro-Riggers, J.2
Bommarius, A.S.3
-
15
-
-
66249122803
-
Inferring stabilizing mutations from protein phylogenies: application to influenza hemagglutinin
-
Bloom J.D., Glassman M.J. Inferring stabilizing mutations from protein phylogenies: application to influenza hemagglutinin. PLoS Comput. Biol. 2009, 5:e1000349.
-
(2009)
PLoS Comput. Biol.
, vol.5
-
-
Bloom, J.D.1
Glassman, M.J.2
-
16
-
-
0032437592
-
Semirational design of active tumor suppressor p53 DNA binding domain with enhanced stability
-
Nikolova P.V., Henckel J., Lane D.P., Fersht A.R. Semirational design of active tumor suppressor p53 DNA binding domain with enhanced stability. Proc. Natl Acad. Sci. USA 1998, 95:14675-14680.
-
(1998)
Proc. Natl Acad. Sci. USA
, vol.95
, pp. 14675-14680
-
-
Nikolova, P.V.1
Henckel, J.2
Lane, D.P.3
Fersht, A.R.4
-
17
-
-
0034495088
-
The design of a hyperstable mutant of the Abp1p SH3 domain by sequence alignment analysis
-
Rath A., Davidson A.R. The design of a hyperstable mutant of the Abp1p SH3 domain by sequence alignment analysis. Protein Sci. 2000, 9:2457-2469.
-
(2000)
Protein Sci.
, vol.9
, pp. 2457-2469
-
-
Rath, A.1
Davidson, A.R.2
-
18
-
-
33748101201
-
Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output
-
Loening A.M., Fenn T.D., Wu A.M., Gambhir S.S. Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output. Protein Eng. Des. Sel. 2006, 19:391-400.
-
(2006)
Protein Eng. Des. Sel.
, vol.19
, pp. 391-400
-
-
Loening, A.M.1
Fenn, T.D.2
Wu, A.M.3
Gambhir, S.S.4
-
19
-
-
0036271498
-
The consensus concept for thermostability engineering of proteins: further proof of concept
-
Lehmann M., Loch C., Middendorf A., Studer D., Lassen S.F., Pasamontes L., et al. The consensus concept for thermostability engineering of proteins: further proof of concept. Protein Eng. 2002, 15:403-411.
-
(2002)
Protein Eng.
, vol.15
, pp. 403-411
-
-
Lehmann, M.1
Loch, C.2
Middendorf, A.3
Studer, D.4
Lassen, S.F.5
Pasamontes, L.6
-
20
-
-
47749142434
-
Consensus mutagenesis reveals that non-helical regions influence thermal stability of horseradish peroxidase
-
Ryan B.J., O'Connell M.J., O'Fagain C. Consensus mutagenesis reveals that non-helical regions influence thermal stability of horseradish peroxidase. Biochimie 2008, 90:1389-1396.
-
(2008)
Biochimie
, vol.90
, pp. 1389-1396
-
-
Ryan, B.J.1
O'Connell, M.J.2
O'Fagain, C.3
-
21
-
-
14644390952
-
Construction of stabilized proteins by combinatorial consensus mutagenesis
-
Amin N., Liu A.D., Ramer S., Aehle W., Meijer D., Metin M., et al. Construction of stabilized proteins by combinatorial consensus mutagenesis. Protein Eng. Des. Sel. 2004, 17:787-793.
-
(2004)
Protein Eng. Des. Sel.
, vol.17
, pp. 787-793
-
-
Amin, N.1
Liu, A.D.2
Ramer, S.3
Aehle, W.4
Meijer, D.5
Metin, M.6
-
22
-
-
34948815009
-
A diverse family of thermostable cytochrome P450s created by recombination of stabilizing fragments
-
Li Y.G., Drummond D.A., Sawayama A.M., Snow C.D., Bloom J.D., Arnold F.H. A diverse family of thermostable cytochrome P450s created by recombination of stabilizing fragments. Nat. Biotechnol. 2007, 25:1051-1056.
-
(2007)
Nat. Biotechnol.
, vol.25
, pp. 1051-1056
-
-
Li, Y.G.1
Drummond, D.A.2
Sawayama, A.M.3
Snow, C.D.4
Bloom, J.D.5
Arnold, F.H.6
-
23
-
-
0029109922
-
Atomic structure of the buried catalytic pocket of Escherichia coli chorismate mutase
-
Lee A.Y., Karplus P.A., Ganem B., Clardy J. Atomic structure of the buried catalytic pocket of Escherichia coli chorismate mutase. J. Am. Chem. Soc. 1995, 117:3627-3628.
-
(1995)
J. Am. Chem. Soc.
, vol.117
, pp. 3627-3628
-
-
Lee, A.Y.1
Karplus, P.A.2
Ganem, B.3
Clardy, J.4
-
24
-
-
33845983675
-
Relative tolerance of mesostable and thermostable protein homologs to extensive mutation
-
Besenmatter W., Kast P., Hilvert D. Relative tolerance of mesostable and thermostable protein homologs to extensive mutation. Proteins 2007, 66:500-506.
-
(2007)
Proteins
, vol.66
, pp. 500-506
-
-
Besenmatter, W.1
Kast, P.2
Hilvert, D.3
-
25
-
-
23444436172
-
Protein design by binary patterning of polar and nonpolar amino acids
-
Kamtekar S., Schiffer J.M., Xiong H., Babik J.M., Hecht M.H. Protein design by binary patterning of polar and nonpolar amino acids. Science 1993, 262:1680-1685.
-
(1993)
Science
, vol.262
, pp. 1680-1685
-
-
Kamtekar, S.1
Schiffer, J.M.2
Xiong, H.3
Babik, J.M.4
Hecht, M.H.5
-
26
-
-
0032516484
-
A small, thermostable, and monofunctional chorismate mutase from the archeon Methanococcus jannaschii
-
MacBeath G., Kast P., Hilvert D. A small, thermostable, and monofunctional chorismate mutase from the archeon Methanococcus jannaschii. Biochemistry 1998, 37:10062-10073.
-
(1998)
Biochemistry
, vol.37
, pp. 10062-10073
-
-
MacBeath, G.1
Kast, P.2
Hilvert, D.3
-
27
-
-
0035845571
-
Searching sequence space for protein catalysts
-
Taylor S.V., Walter K.U., Kast P., Hilvert D. Searching sequence space for protein catalysts. Proc. Natl Acad. Sci. USA 2001, 98:10596-10601.
-
(2001)
Proc. Natl Acad. Sci. USA
, vol.98
, pp. 10596-10601
-
-
Taylor, S.V.1
Walter, K.U.2
Kast, P.3
Hilvert, D.4
-
28
-
-
67549086082
-
Understanding the functional roles of amino acid residues in enzyme catalysis
-
Holliday G.L., Mitchell J.B.O., Thornton J.M. Understanding the functional roles of amino acid residues in enzyme catalysis. J. Mol. Biol. 2009, 390:560-577.
-
(2009)
J. Mol. Biol.
, vol.390
, pp. 560-577
-
-
Holliday, G.L.1
Mitchell, J.B.O.2
Thornton, J.M.3
-
29
-
-
0025082684
-
Additivity of mutational effects in proteins
-
Wells J.A. Additivity of mutational effects in proteins. Biochemistry 1990, 29:8509-8517.
-
(1990)
Biochemistry
, vol.29
, pp. 8509-8517
-
-
Wells, J.A.1
-
30
-
-
4744365127
-
Beyond consensus: statistical free energies reveal hidden interactions in the design of a TPR motif
-
Magliery T.J., Regan L. Beyond consensus: statistical free energies reveal hidden interactions in the design of a TPR motif. J. Mol. Biol. 2004, 343:731-745.
-
(2004)
J. Mol. Biol.
, vol.343
, pp. 731-745
-
-
Magliery, T.J.1
Regan, L.2
|