Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output

Protein Engineering, Design and Selection

Volumn 19, Issue 9, 2006, Pages 391-400

  Loening, Andreas Markus ^b,c   Fenn, Timothy David ^b   Wu, Anna M ^a   Gambhir, Sanjiv Sam ^a,b,c,d  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b STANFORD UNIVERSITY   (United States)

^c Stanford University   (United States)

^d STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Enzyme stability; Mutagenesis; Renilla luciferase; Reporter gene

Indexed keywords

ENZYME STABILITY; LUCIFERASE PROTEIN; RENILLA LUCIFERASE; REPORTER GENE;

BIOASSAY; BIOLUMINESCENCE; BODY FLUIDS; GENES; MEDICAL IMAGING; MOLECULAR STRUCTURE; PROTEINS;

MUTAGENESIS;

LIGAND; MUTANT PROTEIN; RENILLA LUCIFERIN 2 MONOOXYGENASE;

ARTICLE; BIOLOGY; BIOLUMINESCENCE; CONJUGATE; CONSENSUS SEQUENCE; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME STABILITY; IMAGING; LIGHT; MOLECULAR PROBE; MUTAGENESIS; MUTATION; MUTATIONAL ANALYSIS; PRIORITY JOURNAL; REPORTER GENE; SENSITIVITY ANALYSIS; SERUM;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; DNA MUTATIONAL ANALYSIS; LUCIFERASES, RENILLA; LUMINESCENCE; MODELS, GENETIC; MODELS, MOLECULAR; MOLECULAR PROBE TECHNIQUES; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; RENILLA; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA;

ANIMALIA; MURINAE; RENILLA LUCIFERASE;

EID: 33748101201     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzl023     Document Type: Article

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