The modified high-density survival assay is the useful tool to predict the effectiveness of fractionated radiation exposure

Journal of Radiation Research

Volumn 51, Issue 3, 2010, Pages 297-302

  Kuwahara, Yoshikazu ^a   Mori, Miyuki ^a   Oikawa, Toshiyuki ^a   Shimura, Tsutomu ^a   Ohtake, Yosuke ^b   Mori, Shiro ^a   Ohkubo, Yasuhito ^b   Fukumoto, Manabu ^a  

^a TOHOKU UNIVERSITY   (Japan)

^b TOHOKU PHARMACEUTICAL UNIVERSITY   (Japan)

Author keywords

Clinically relevant radioresistance; Clonogenic assay; Ionizing radiation; Modified high density survival assay; MTT assay

Indexed keywords

CELL CULTURE; CELLS;

CANCER CELLS; CELL RESPONSE; CLINICALLY RELEVANT RADIORESISTANCE; CLONOGENIC ASSAYS; FRACTIONATED RADIATION; MODIFIED HIGH-DENSITY SURVIVAL ASSAY; MTT ASSAYS; RADIATION EXPOSURE; RADIORESISTANCE; SURVIVAL ASSAYS;

IONIZING RADIATION;

TETRAZOLIUM; THIAZOLE DERIVATIVE; THIAZOLYL BLUE;

ANIMAL; CANCER TRANSPLANTATION; CELL SURVIVAL; HUMAN; K562 CELL LINE; MITOSIS; MOUSE; NUDE MOUSE; PROCEDURES; RADIATION DOSE; RADIATION DOSE FRACTIONATION; RADIATION RESPONSE; RADIOTHERAPY; TUMOR CELL LINE; U937 CELL LINE; X RAY;

ANIMALS; CELL LINE, TUMOR; CELL SURVIVAL; DOSE FRACTIONATION; DOSE-RESPONSE RELATIONSHIP, RADIATION; HUMANS; K562 CELLS; MICE; MICE, NUDE; MITOSIS; NEOPLASM TRANSPLANTATION; RADIATION TOLERANCE; RADIOTHERAPY; TETRAZOLIUM SALTS; THIAZOLES; U937 CELLS; X-RAYS;

EID: 77953990955     PISSN: 04493060     EISSN: 13499157     Source Type: Journal    
DOI: 10.1269/jrr.09094     Document Type: Article

References (19)

1. Aldridge DR and Radford IR (1998) Explaining differences in sensitivity to killing by ionizing radiation between human lymphoid cell lines. Cancer Res 58: 2817-2824.
2. Hall EJ and Giaccia AJ (2006) Radiobiology for the radiologist. Philadelphia: Lippincott Williams & Wilkins.
3. Pomp J, et al (1996) Cell density dependent plating efficiency affects outcome and interpretation of colony forming assays. Radiother Oncol 40: 121-125.
4. Fitzek M and Trott KR (1993) Clonal heterogeneity in delayed decrease of plating efficiency of irradiated HeLa cells. Radiat Environ Biophys 32: 33-39.
5. Liu ZZ, et al (2005) Cell cycle and radiosensitivity of progeny of irradiated primary cultured human hepatocarcinoma cells. World J Gastroenterol 11: 7033-7035.
6. Lichter AS and Lawrence TS (1995) Recent advances in radiation oncology. N Engl J Med 332: 371-379.
7. Gudkov AV and Komarova EA (2003) The role of p53 in determining sensitivity to radiotherapy. Nat Rev Cancer 3: 117-129.
8. Roninson IB, Broude EV and Chang BD (2001) If not apop-tosis, then what? Treatment-induced senescence and mitotic catastrophe in tumor cells. Drug Resist Updat 4: 303-313.
9. Roninson IB (2003) Tumor cell senescence in cancer treatment. Cancer Res 63: 2705-2715.
10. Castedo M, et al (2004) Cell death by mitotic catastrophe: a molecular definition. Oncogene 23: 2825-2837.
11. Shay JW and Roninson IB (2004) Hallmarks of senescence in carcinogenesis and cancer therapy. Oncogene 23: 2919-2933.
12. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotox-icity assays. J Immunol Methods 65: 55-63.
13. Mirzayans R, et al (2007) A sensitive assay for the evaluation of cytotoxicity and its pharmacologic modulation in human solid tumor-derived cell lines exposed to cancer-therapeutic agents. J Pharm Pharm Sci 10: 298s-311s.
14. Kuwahara Y, et al (2009) Clinically relevant radioresistant cells efficiently repair DNA double-strand breaks induced by X-rays. Cancer Sci 100: 747-752.
15. Wang L, et al (2007) Analysis of Common Deletion (CD) and a novel deletion of mitochondrial DNA induced by ionizing radiation. Int J Radiat Biol 83: 433-442.
16. Brown JM and Wouters BG (1999) Apoptosis, p53, and tumor cell sensitivity to anticancer agents. Cancer Res 59: 1391-1399.
17. Uchida H, et al (2004) Adenovirus-mediated transfer of siRNA against survivin induced apoptosis and attenuated tumor cell growth in vitro and in vivo. Mol Ther 10: 162-171.
18. Hulspas R, et al (1997) In vitro cell density-dependent clonal growth of EGF-responsive murine neural progenitor cells under serum-free conditions. Exp Neurol 148: 147-156.
19. West CM, et al (1998) The intrinsic radiosensitivity of normal and tumour cells. International journal of radiation biology 73: 409-413.