Detecting extracellular carbonic anhydrase activity using membrane inlet mass spectrometry

Analytical Biochemistry

Volumn 403, Issue 1-2, 2010, Pages 74-78

  Delacruz, Joannalyn ^a   Mikulski, Rose ^a   Tu, Chingkuang ^a   Li, Ying ^a   Wang, Hai ^a   Shiverick, Kathleen T ^a   Frost, Susan C ^a   Horenstein, Nicole A ^a   Silverman, David N ^a  

^a UNIVERSITY OF FLORIDA   (United States)

Author keywords

Carbon dioxide; Carbonic anhydrase; Enzyme activity; Mass spectrometry; Stable isotope

Indexed keywords

CARBON DIOXIDE; CELLS; CYTOLOGY; DISEASES; ENZYME ACTIVITY; MASS SPECTROMETRY; PHYSIOLOGY;

CELL PHYSIOLOGY; EXTRACELLULAR ACIDIFICATIONS; MALIGNANT TUMORS; MEMBRANE INLET MASS SPECTROMETRY; MEMBRANE-IMPERMEANT; PROSTATE CANCER CELLS; SPECTROMETRIC METHODS; STABLE ISOTOPES;

CARBONIC ANHYDRASE;

CARBON DIOXIDE; CARBONATE DEHYDRATASE; CARBONATE DEHYDRATASE IX; MACROGOL; MAFENIDE; OXYGEN 18; CARBONATE DEHYDRATASE INHIBITOR; MACROGOL DERIVATIVE;

ARTICLE; CANCER CELL; CELL MEMBRANE PERMEABILITY; CELL SUSPENSION; CONTROLLED STUDY; ENZYME ACTIVITY; ERYTHROCYTE; EXTRACELLULAR SPACE; HUMAN; HUMAN CELL; MASS SPECTROMETRY; PRIORITY JOURNAL; BREAST TUMOR; CHEMISTRY; ENZYMOLOGY; EVALUATION STUDY; FEMALE; MALE; METABOLISM; PROCEDURES; PROSTATE TUMOR; SENSITIVITY AND SPECIFICITY; TUMOR CELL LINE;

BREAST NEOPLASMS; CARBON DIOXIDE; CARBONIC ANHYDRASE INHIBITORS; CARBONIC ANHYDRASES; CELL LINE, TUMOR; ERYTHROCYTES; EXTRACELLULAR SPACE; FEMALE; HUMANS; MALE; MASS SPECTROMETRY; POLYETHYLENE GLYCOLS; PROSTATIC NEOPLASMS; SENSITIVITY AND SPECIFICITY;

EID: 77953363581     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.04.019     Document Type: Article

References (32)

1. Lindskog S. Structure and mechanism of carbonic anhydrase. Pharmacol. Ther. 1997, 74:1-20.
2. Silverman D.N., Lindskog S. The catalytic mechanism of carbonic anhydrase: implications of a rate-limiting protolysis of water. Acc. Chem. Res. 1988, 21:30-36.
3. Chegwidden W.R., Carter N.D., Edwards Y.H. The Carbonic Anhydrases: New Horizons 2000, Birkhäuser Verlag, Basel, Switzerland.
4. Dorai T., Sawczuk I.S., Pastorek J., Wiernik P.H., Dutcher J.P. The role of carbonic anhydrase IX overexpression in kidney cancer. Eur. J. Cancer 2005, 41:2935-2947.
5. De Simone G., Supuran C.T. Carbonic anhydrase IX: biochemical and crystallographic characterization of a novel antitumor target. Biochim. Biophys. Acta Proteins Proteomics 2010, 1804:404-409.
6. Li Y., Wang H., Oosterwijk E., Selman Y., Mira J.C., Medrano T., Shiverick K.T., Frost S.C. Antibody-specific detection of CA IX in breast and prostate cancers. Biochem. Biophys. Res. Commun. 2009, 386:488-492.
7. Svastova E., Hulikova A., Rafajova M., Zat'ovicova M., Gibadulinova A., Casini A., Cecchi A., Scozzafava A., Supuran C.T., Pastorek J., Pastorekova S. Hypoxia activates the capacity of tumor-associated carbonic anhydrase IX to acidify extracellular pH. FEBS Lett. 2004, 577:439-445.
8. Swietach P., Patiar S., Supuran C.T., Harris A.L., Vaughan-Jones R.D. The role of carbonic anhydrase 9 in regulating extracellular and intracellular pH in three-dimensional tumor cell growths. J. Biol. Chem. 2009, 284:20299-20310.
9. Swietach P., Wigfield S., Cobden P., Supuran C.T., Harris A.L., Vaughan-Jones R.D. Tumor-associated carbonic anhydrase 9 spatially coordinates intracellular pH in three-dimensional multicellular growths. J. Biol. Chem. 2008, 283:20473-20483.
10. Supuran C.T., Scozzafava A., Conway J. Carbonic Anhydrase: Its Inhibitors and Activators 2004, CRC Press, Boca Raton, FL.
11. Supuran C.T. Carbonic anhydrases: novel therapeutic applications for inhibitors and activators. Nat. Rev. Drug Disc. 2008, 7:168-181.
12. Wingo T., Tu C., Laipis P.J., Silverman D.N. The catalytic properties of human carbonic anhydrase IX. Biochem. Biophys. Res. Commun. 2001, 288:666-669.
13. Hilvo M., Baranauskiene L., Salzano A., Scaloni A., Matulis D., Innocenti A., Scozzafava A., Monti S., Di Fiore A., De Simone G., Lindfors M., Janis J., Valjakka J., Pastorekova S., Pastorek J., Kulomaa M., Nordlund H., Supuran C., Parkkila S. Biochemical characterization of carbonic anhydrase IX, one of the most active carbonic anhydrase isozymes. J. Biol. Chem. 2008, 283:27799-27809.
14. Conroy C.W., Wynns G.C., Maren T.H. Synthesis and properties of two new membrane-impermeant high-molecular-weight carbonic anhydrase inhibitors. Bioorg. Chem. 1996, 24:262-272.
15. Hoch G., Kok B. A mass spectrometer inlet system for sampling gases dissolved in liquid phases. Arch. Biochem. Biophys. 1963, 101:160-170.
16. Silverman D.N. Carbonic anhydrase: oxygen-18 exchange catalyzed by an enzyme with rate-contributing proton-transfer steps. Methods Enzymol. 1982, 87:732-752.
17. Lauritsen F.R., Kotiaho T., Choudhury T.K., Cooks R.G. Direct detection and identification of volatile organic compounds dissolved in organic solvents by reversed-phase membrane introduction tandem mass spectrometry. Anal. Chem. 1992, 64:1205-1211.
18. An H., Tu C., Duda D., Montanez-Clemente I., Math K., Laipis P.J., McKenna R., Silverman D.N. Chemical rescue in catalysis by human carbonic anhydrases II and III. Biochemistry 2002, 41:3235-3242.
19. Khalifah R.G., Strader D.J., Bryant S.H., Gibson S.M. C-13 nuclear magnetic resonance probe of active-site ionizations in human carbonic anhydrase B. Biochemistry 1977, 16:2241-2247.
20. Fishman A., Acton A., Lee-Ruff E. A simple preparation of PEG-carboxylates by direct oxidation. Synth. Commun. 2004, 34:2309-2312.
21. Johansson G., Gysin R., Flanagan S.D. Affinity partitioning of membranes: evidence for discrete membrane domains containing cholinergic receptor. J. Biol. Chem. 1981, 256:9126-9135.
22. Handayani R., Rice L., Cui Y.H., Medrano T.A., Samedi V.G., Baker H.V., Szabo N.J., Shiverick K.T. Soy isoflavones alter expression of genes associated with cancer progression, including interleukin-8, in androgen-independent PC-3 human prostate cancer cells. J. Nutr. 2006, 136:75-82.
23. Ivanov S., Liao S.Y., Ivanova A., Danilkovitch-Miagkova A., Tarasova N., Weirich G., Merrill M.J., Proescholdt M.A., Oldfield E.H., Lee J., Zavada J., Waheed A., Sly W., Lerman M.I., Stanbridge E.J. Expression of hypoxia-inducible cell-surface transmembrane carbonic anhydrases in human cancer. Am. J. Pathol. 2001, 158:905-919.
24. Mills G.A., Urey H.C. The kinetics of isotopic exchange between carbon dioxide, bicarbonate ion, carbonate ion, and water. J. Am. Chem. Soc. 1940, 62:1019-1026.
25. Silverman D.N., Tu C.K., Lindskog S., Wynns G.C. Rate of exchange of water from the active site of human carbonic anhydrase C. J. Am. Chem. Soc. 1979, 101:6734-6740.
26. 18O exchange. J. Biol. Chem. 1978, 253:8178-8184.
27. 18O exchange. J. Biol. Chem. 1977, 252:3881-3890.
28. Alterio V., Hilvo M., Di Fiore A., Supuran C.T., Pan P.W., Parkkila S., Scaloni A., Pastorek J., Pastorekova S., Pedone C., Scozzafava A., Monti S.M., De Simone G. Crystal structure of the catalytic domain of the tumor-associated human carbonic anhydrase IX. Proc. Natl. Acad. Sci. USA 2009, 106:16233-16238.
29. Maren T.H., Sanyal G. The activity of sulfonamides and anions against the carbonic anhydrases of animals, plants, and bacteria. Annu. Rev. Pharmacol. Toxicol. 1983, 23:439-459.
30. 18O exchange. Isotopes Environ. Health Stud. 1997, 33:197-205.
31. Wilbur K.M., Anderson N.G. Electrometric and colorimetric determination of carbonic anhydrase. J. Biol. Chem. 1948, 176:147-154.
32. Khalifah R.G. Carbon dioxide hydration activity of carbonic anhydrase: I. Stop-flow kinetic studies on native human isoenzyme B and isoenzyme C. J. Biol. Chem. 1971, 246:2561-2573.