Rac1 modulates TGF-β1-mediated epithelial cell plasticity and MMP9 production in transformed keratinocytes

FEBS Letters

Volumn 584, Issue 11, 2010, Pages 2305-2310

  Santibáñez, Juan F ^a,b   Kocić, Jelena ^b   Fabra, Angels ^c   Cano, Amparo ^d   Quintanilla, Miguel ^d  

^a UNIVERSITY OF CHILE   (Chile)

^b UNIVERSITY OF BELGRADE   (Serbia)

^c BELLVITGE BIOMEDICAL RESEARCH INSTITUTE IDIBELL   (Spain)

^d UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

Author keywords

Epithelial mesenchymal transition; Keratinocyte; Metalloproteinase 9; Migration; Mitogen activated protein kinase; Rac1; Snail1; Transforming growth factor 1

Indexed keywords

2 (2 AMINO 3 METHOXYPHENYL)CHROMONE; GELATINASE B; GUANOSINE TRIPHOSPHATASE; MITOGEN ACTIVATED PROTEIN KINASE; RAC1 PROTEIN; TRANSCRIPTION FACTOR SNAIL; TRANSFORMING GROWTH FACTOR BETA1; UVOMORULIN;

ANIMAL CELL; ARTICLE; CELL INVASION; CELL MIGRATION; CELL STRUCTURE; CELL TRANSFORMATION; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME SYNTHESIS; EPITHELIAL MESENCHYMAL TRANSITION; EPITHELIUM CELL; GENE MUTATION; KERATINOCYTE; MOUSE; NONHUMAN; PLASTICITY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SECRETION; SIGNAL TRANSDUCTION;

ANIMALS; CADHERINS; CELL DEDIFFERENTIATION; CELL MOVEMENT; ENZYME INHIBITORS; EPITHELIAL CELLS; FLAVONOIDS; KERATINOCYTES; MATRIX METALLOPROTEINASE 9; MICE; MITOGEN-ACTIVATED PROTEIN KINASES; SIGNAL TRANSDUCTION; TRANSFORMING GROWTH FACTOR BETA1;

EID: 77952956960     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2010.03.042     Document Type: Article

References (30)

1. Xu J., Lamouille S., Derynck R. TGF-beta-induced epithelial to mesenchymal transition. Cell Res. 2009, 19:156-172.
2. Yang J., Weinberg R.A. Epithelial-mesenchymal transition: at the crossroads of development and tumor metastasis. Dev. Cell 2008, 14:818-829.
3. Kardassis D., Murphy C., Fotsis T., Moustakas A., Stournaras C. Control of transforming growth factor β signal transduction by small GTPases. FEBS J. 2009, 276:2947-2965.
4. Vega F.M., Ridley A.J. Rho GTPases in cancer cell biology. FEBS Lett. 2008, 582:2093-2101.
5. Caulin C., Scholl F.G., Frontelo P., Gamallo C., Quintanilla M. Chronic exposure of cultured transformed mouse epidermal cells to transforming growth factor-beta 1 induces an epithelial-mesenchymal transdifferentiation and a spindle tumoral phenotype. Cell Growth Differ. 1995, 6:1027-1035.
6. Cui W., Fowlis D.J., Bryson S., Duffie E., Ireland H., Balmain A., Akhurst R.J. TGFbeta1 inhibits the formation of benign skin tumors, but enhances progression to invasive spindle carcinomas in transgenic mice. Cell 1996, 86:531-542.
7. Frontelo P., González-Garrigues M., Vilaró S., Gamallo C., Fabra A., Quintanilla M. Transforming growth factor β1 induces squamous carcinoma cell variants with increased metastatic abilities and a disorganized cytoskeleton. Exp. Cell Res. 1998, 244:420-432.
8. Santibáñez J.F., Frontelo P., Iglesias M., Martinez J., Quintanilla M. Urokinase expression and binding activity associated with the transforming growth factor β1-induced migratory and invasive phenotype of mouse epidermal keratinocytes. J. Cell. Biochem. 1999, 74:61-73.
9. Santibáñez J.F., Iglesias M., Frontelo P., Martinez J., Quintanilla M. Involvement of the Ras/MAPK signaling pathway in the modulation of urokinase production and cellular invasiveness by transforming growth factor-β1 in transformed keratinocytes. Biochem. Biophys. Res. Commun. 2000, 273:521-527.
10. Santibáñez J.F., Guerrero J., Quintanilla M., Fabra A., Martinez J. Transforming growth factor-β1 modulates matrix metalloproteinase-9 production through the Ras/MAPK signaling pathway in transformed keratinocytes. Biochem. Biophys. Res. Commun. 2002, 296:267-273.
11. Deryugina E.I., Quigley J.P. Matrix metalloproteinases and tumor metastasis. Cancer Metastasis Rev. 2006, 25:9-34.
12. Yu Q., Stamenkovic I. Cell surface-localized matriz metalloproteinase-9 proteolytically activates TGF-β and promotes tumor invasion and angiogenesis. Genes Dev. 2000, 14:163-176.
13. Coso A.O., Chiariello M., Yu J.-C., Teramoto H., Crespo P., Xu N., Miki T., Gutkind J.S. The small GTP-binding proteins rac1 and Cdc42 regulate the activity of the JNK/SAPK signalling pathway. Cell 1995, 81:1137-1146.
14. Llorens A., Rodrigo I., López-Barcons L., González-Garrigues M., Lozano E., Vinyals A., Quintanilla M., Cano A., Fabra A. Down-regulation of E-cadherin in mouse skin carcinoma cells enhances a migratory and invasive phenotype linked to matriz metalloproteinase-9 gelatinase expression. Lab. Invest. 1998, 78:1131-1142.
15. Moreno-Bueno G., Peinado H., Molina P., Olmeda D., Cubillo E., Santos V., Palacios J., Portillo F., Cano A. The morphological and molecular features of the epithelial-mesenchymal transition. Nat. Protoc. 2009, 4:1591-1613.
16. Martin-Villar E., Megias D., Castel S., Yurrita M.M., Vilaró S., Quintanilla M. Podoplanin binds ERM proteins to activate RhoA and promote epithelial-mesenchymal transition. J. Cell Sci. 2006, 119:4541-4553.
17. Iglesias M., Frontelo P., Gamallo C., Quintanilla M. Blockade of Smad4 in transformed keratinocytes containing a Ras oncogene leads to hyperactivation of the Ras-dependent Erk signaling pathway associated with progression to undifferentiated carcinomas. Oncogene 2000, 19:4134-4145.
18. Ridley A.J. Rho GTPases and cell migration. J. Cell Sci. 2001, 114:2713-2722.
19. Peinado H., Olmeda D., Cano A. Snail, ZEB and bHLH factors in tumour progression: an alliance against the epithelial phenotype?. Nat. Rev. Cancer 2007, 7:415-428.
20. Quintanilla M., Frontelo P., Pons M., Romero D., Pérez-Gómez E., Gamallo C., Iglesias M. TGF-β signaling and epidermal carcinogenesis. Recent Res. Devel. Cell Biochem. 2003, 1:151-160.
21. Bhowmick N.A., Ghiassi M., Bakin A., Aakre M., Lundquist C.A., Engel M.E., Arteaga C.L., Moses H.L. Transforming growth factor-β1 mediates epithelial to mesenchymal transdifferentiation through a RhoA-dependent mechanism. Mol. Biol. Cell 2001, 12:27-36.
22. Edlund S., Landstrom M., Heldin C.H., Aspenstrom P. Transforming growth factor-β-induced mobilization of actin cytoskeleton requires signalling by small GTPases Cdc42 and RhoA. Mol. Biol. Cell 2002, 13:902-914.
23. Cho H.J., Yoo J. Rho activation is required for transforming growth factor-β-induced epithelial-mesenchymal transition in lens epithelial cells. Cell Biol. Int. 2007, 31:1225-1230.
24. Sanz-Moreno V., Gadea G., Ahn J., Paterson H., Marra P., Pinner S., Sahai E., Marshall C.J. Rac activation and inactivation control plasticity of tumor cell movement. Cell 2008, 135:510-523.
25. Seomun Y., Kim J.T., Joo C.K. MMP-14 mediated MMP-9 expression is involved in TGF-β1-induced keratinocyte migration. J. Cell. Biochem. 2008, 104:934-941.
26. Sinpitaksakul S.N., Pimkhaokham A., Sanchavanakit N., Pavasant P. TGF-β1 induced MMP-9 expression in HNSCC cell lines via Smad/MLCK pathway. Biochem. Biophys. Res. Commun. 2008, 371:713-718.
27. Chou Y.-T., Wang H., Chen Y., Danielpour D., Yang Y.-C. Cited 2 modulates TGF-β-mediated upregulation of MMP9. Oncogene 2006, 25:5547-5560.
28. Sun L., Diamond M.E., Ottaviano A.J., Joseph M.J., Ananthanarayan V., Munshi H.G. Transforming growth factor-β1 promotes matrix metalloproteinase-9-mediated oral cancer invasion through Snail expression. Mol. Cancer Res. 2008, 6:10-20.
29. Aznar S., Lacal J.C. Rho signals to cell growth and apoptosis. Cancer Lett. 2001, 165:1-10.
30. Peinado H., Quintanilla M., Cano A. Transforming growth factor beta-1 induces snail transcription factor in epithelial cell lines: mechanisms for epithelial-mesenchymal transitions. J. Biol. Chem. 2003, 278:21113-21123.