Simultaneous detection of intracellular target and off-target binding of small molecule cancer drugs at nanomolar concentrations

British Journal of Pharmacology

Volumn 160, Issue 4, 2010, Pages 958-970

  Glauner, Heike ^a   Ruttekolk, Ivo R ^a   Hansen, Kerrin ^b   Steemers, Ben ^a   Chung, Yi Da ^a   Becker, Frank ^b   Hannus, Stefan ^b   Brock, Roland ^a  

^a RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

^b Intana Bioscience GmbH   (Germany)

Author keywords

Anti cancer drug; Cellular pharmacology; Drug development; Drug target interaction; Fluorescence correlation spectroscopy; Fluorescence cross correlation spectroscopy

Indexed keywords

ABELSON KINASE; ANTINEOPLASTIC AGENT; DIHYDROFOLATE REDUCTASE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MACROGOL; METHOTREXATE; PD 173956; PROTEIN KINASE INHIBITOR; PURVALANOL B; UNCLASSIFIED DRUG; ADENINE; FLUORESCENT DYE; FOLIC ACID ANTAGONIST; MACROGOL DERIVATIVE; PYRIDONE DERIVATIVE; PYRIMIDINE DERIVATIVE;

ARTICLE; CANCER CELL; CELL STRAIN HEK293; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; DRUG BINDING; DRUG CONJUGATION; DRUG DISTRIBUTION; DRUG SYNTHESIS; FLUORESCENCE CORRELATION SPECTROSCOPY; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; MOLECULAR INTERACTION; PRIORITY JOURNAL; ABSORPTION; ANALOGS AND DERIVATIVES; CELL LINE; CHEMICAL PHENOMENA; CHEMISTRY; DRUG SCREENING; FEMALE; GENETICS; HELA CELL LINE; LASER SCANNING CYTOMETRY; METABOLISM; MULTIPHOTON MICROSCOPY; PROCEDURES;

ABSORPTION; ADENINE; ANTINEOPLASTIC AGENTS; CELL LINE; DRUG SCREENING ASSAYS, ANTITUMOR; FEMALE; FLUORESCENT DYES; FOLIC ACID ANTAGONISTS; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; LASER SCANNING CYTOMETRY; METHOTREXATE; MICROSCOPY, FLUORESCENCE, MULTIPHOTON; POLYETHYLENE GLYCOLS; PROTEIN KINASE INHIBITORS; PROTO-ONCOGENE PROTEINS C-ABL; PYRIDONES; PYRIMIDINES; RECOMBINANT FUSION PROTEINS; TETRAHYDROFOLATE DEHYDROGENASE;

EID: 77952843524     PISSN: 00071188     EISSN: 14765381     Source Type: Journal    
DOI: 10.1111/j.1476-5381.2010.00732.x     Document Type: Article

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