Anomalous diffusion of fluorescent probes inside living cell investigated by spatially-resolved fluorescence correlation spectroscopy

Journal of Molecular Biology

Volumn 298, Issue 4, 2000, Pages 677-689

  Wachsmuth, Malte ^a   Waldeck, Waldemar ^a   Langowski, Jörg ^a  

^a GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

Anomalous diffusion; EGFP; Fluorescence correlation spectroscopy; Intracellular diffusion; Nuclear structure

Indexed keywords

GREEN FLUORESCENT PROTEIN; MUTANT PROTEIN;

ANIMAL CELL; AQUEOUS SOLUTION; ARTICLE; BINDING AFFINITY; CELL NUCLEUS; CELLULAR DISTRIBUTION; CHROMATOPHORE; CHROMOSOME STRUCTURE; CONTROLLED STUDY; FLUORESCENCE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; SPECTROSCOPY;

ANIMALIA;

EID: 0034640133     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3692     Document Type: Article

References (52)

1. Two-photon fluorescence correlation spectroscopy: Method and application to the intracellular environment
2. Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein
3. Fluorescence correlation microscopy (FCM): Fluorescence correlation spectroscopy (FCS) taken into the cell
4. Fluorescence correlation microscopy of cells in the presence of autofluorescence
5. Ultra-fast excited state dynamics in green fluorescent protein: Multiple states and proton transfer
6. Role of chromosome territories in the functional compartmentalization of the cell nucleus
7. On/off blinking and switching behaviour of single molecules of green fluorescent protein
8. Sorting single molecules: Application to ,diagnostics and evolutionary biotechnology
9. Fluorescence correlation spectroscopy. I. Conceptual basis and theory
10. Direct observation in the millisecond time range of fluorescent molecule asymmetrical interaction with the electropermeabilized cell membrane
11. Visualising individual green fluorescent proteins with a near field optical microscope
12. Dynamics of fluorescence fluctuations in green fluorescent protein observed by fluorescence correlation spectroscopy
13. Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells
14. Determinants of the translational mobility of a small solute in cell cytoplasm
15. Statistical accuracy in fluorescence correlation spectroscopy
16. Determination of DNA-ligand interactions by fluorescence correlation spectroscopy
17. Probing the structure of cytoplasm
18. Hindered diffusion of inert tracer particles in the cytoplasm of mouse 3T3 cells
19. A novel fluorescence ratiometric method confirms the low solvent viscosity of the cytoplasm
20. Thermodynamic fluctuations in a reacting system: Measurement by fluorescence correlation spectroscopy
21. Chromosome structure described by a polymer model
22. Compartmentalization of interphase chromosomes observed in simulation and experiment
23. Long tail kinetics in biophysics?
24. Crystal structure of the Aequorea victoria green fluorescent protein
25. In situ hybridization in living cells: Detection of RNA molecules
26. Green fluorescent protein: Extensive characterization aids the use as a marker protein for many applications
27. The structural basis for spectral variations in green fluorescent protein
28. Rapid diffusion of green fluorescent protein in the mitochondrial matrix
29. Single-molecule fluorescence detection of green fluorescence protein and application to single-protein dynamics. Methods Cell Biol
30. Imaging individual green fluorescent proteins
31. Intranuclear diffusion and hybridization state of oligonucleotides measured by fluorescence correlation spectroscopy in living cells
32. Analysis of confocal laser-microscope optics for 3-D fluorescence correlation spectroscopy
33. Stochastic fractal behavior in concentration fluctuation and fluorescence correlation spectroscopy
34. Fluorescence correlation spectroscopy with high count rate and low background: Analysis of translational diffusion
35. Anomalous diffusion due to obstacles: A Monte Carlo study
36. Single-particle tracking: Effects of corrals
37. Anomalous diffusion due to binding: A Monte Carlo study
38. Time-scale invariance in transport and relaxation
39. Fluorescence correlation spectroscopy with single-molecule sensitivity on cell and model membranes
40. Translational diffusion of macromolecule-sized solutes in cytoplasm and nucleus
41. Cytoplasmic viscosity near the cell plasma membrane: Translational diffusion of a small fluorescent solute measured by total internal reflection-fluorescence photobleaching recovery
42. Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: Cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion
43. Molecular characterisation of recombinant green fluorescent protein by fluorescence correlation microscopy
44. The green fluorescent protein
45. Line-scanning microphotolysis for diffraction-limited measurements of lateral diffusion
46. Fluorescence correlation spectroscopy of triplet states in solution: A theoretical and experimental study
47. The molecular structure of green fluorescent protein
48. Evidence for a nuclear compartment of transcription and splicing located at chromosome domain boundaries