Determination of in vivo dissociation constant, KD, of Cdc42-effector complexes in live mammalian cells using single wavelength fluorescence cross-correlation spectroscopy

Journal of Biological Chemistry

Volumn 284, Issue 20, 2009, Pages 13602-13609

  Sudhaharan, Thankiah ^a   Liu, Ping ^b   Foo, Yong Hwee ^a,b   Bu, Wenyu ^a   Lim, Kim Buay ^a   Wohland, Thorsten ^b   Ahmed, Sohail ^a  

^a INSTITUTE OF MEDICAL BIOLOGY   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

CELL BIOLOGY; CELL CYCLE; CELL POLARITY; CELL SIGNALING; CHINESE HAMSTER OVARY CELLS; DISSOCIATION CONSTANT; EFFECTOR DOMAINS; FLUORESCENCE CROSS-CORRELATION SPECTROSCOPY; GREEN FLUORESCENT PROTEIN; IN-VIVO; INSULIN RECEPTORS; MAMMALIAN CELLS; MEMBRANE-BOUND RECEPTORS; PROTEIN CONCENTRATIONS; PROTEIN INTERACTION; PROTEIN-PROTEIN INTERACTIONS; QUANTITATIVE DATA; RED FLUORESCENT PROTEINS; RESONANCE ENERGY TRANSFER; RHOGTPASE; SINGLE WAVELENGTH; WISKOTT-ALDRICH SYNDROME PROTEINS;

CYTOLOGY; DISSOCIATION; DYES; ENERGY TRANSFER; ENZYME ACTIVITY; FLUORESCENCE; MAMMALS; PROTEINS; SEMICONDUCTOR QUANTUM DOTS; SIGNALING;

CELL MEMBRANES;

CARRIER PROTEINS AND BINDING PROTEINS; GREEN FLUORESCENT PROTEIN; INSULIN RECEPTOR SUBSTRATE; NEURAL WISKOTT ALDRICH SYNDROME PROTEIN; P21 ACTIVATED KINASE; PROTEIN CDC42; RAC PROTEIN; RED FLUORESCENT PROTEIN; UNCLASSIFIED DRUG; WISKOTT ALDRICH SYNDROME PROTEIN;

ANIMAL CELL; ARTICLE; CELL SHAPE; CHO CELL; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DISSOCIATION CONSTANT; FEMALE; FLUORESCENCE CORRELATION SPECTROSCOPY; FLUORESCENCE RESONANCE ENERGY TRANSFER; IN VIVO STUDY; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DETERMINATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; QUANTITATIVE ANALYSIS; SIGNAL TRANSDUCTION; ANIMAL; CRICETULUS; FLUORESCENCE MICROSCOPY; GENETICS; HAMSTER; KINETICS; METABOLISM; METHODOLOGY;

CRICETULUS GRISEUS; MAMMALIA;

ANIMALS; CDC42 GTP-BINDING PROTEIN; CHO CELLS; CRICETINAE; CRICETULUS; FLUORESCENCE RESONANCE ENERGY TRANSFER; GREEN FLUORESCENT PROTEINS; KINETICS; MICROSCOPY, FLUORESCENCE; WISKOTT-ALDRICH SYNDROME PROTEIN;

EID: 67649392701     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M900894200     Document Type: Article

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