Isolation and functional characterization of a new acidic PLA2 Ba SpII RP4 of the Bothrops alternatus snake venom from Argentina

Toxicon

Volumn 56, Issue 1, 2010, Pages 64-74

  Garcia Denegri, María E ^a   Acosta, Ofelia C ^a   Huancahuire Vega, Salomón ^b   Martins de Souza, Daniel ^b,c   Marangoni, Sergio ^b   Maruñak, Silvana L ^a   Teibler, Gladys P ^a   Leiva, Laura C ^a   Ponce Soto, Luis A ^b  

^a UNIVERSIDAD NACIONAL DEL NORDESTE   (Argentina)

^b UNIVERSITY OF CAMPINAS   (Brazil)

^c MAX PLANCK INSTITUTE OF PSYCHIATRY   (Germany)

Author keywords

Acidic Asp49; Amino acid sequence; Bothrops alternatus; Non lethal; Non myotoxic; Phospholipase A2

Indexed keywords

PHOSPHOLIPASE A2; SNAKE VENOM;

ACIDITY; AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARGENTINA; ARTICLE; BIOLOGICAL ACTIVITY; BLOOD CLOTTING TIME; BOTHROPS ALTERNATUS; CONCENTRATION PROCESS; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ENVENOMATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME DEGRADATION; ENZYME ISOLATION; ENZYME KINETICS; GEL FILTRATION; LETHALITY; MALE; MASS SPECTROMETRY; MOLECULAR WEIGHT; MOUSE; MUSCLE INJURY; NONHUMAN; NUCLEOTIDE SEQUENCE; PAW EDEMA; PRIORITY JOURNAL; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SEQUENCE ALIGNMENT; SNAKE; TOXIN ANALYSIS;

ALKYLATION; AMINO ACID SEQUENCE; ANIMALS; ANTICOAGULANTS; ARGENTINA; BOTHROPS; CREATINE KINASE; CROTALID VENOMS; EDEMA; GROUP III PHOSPHOLIPASES A2; HEMOLYTIC AGENTS; HYDROGEN-ION CONCENTRATION; KINETICS; LETHAL DOSE 50; MICE; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MUSCLES; OXIDATION-REDUCTION; PEPTIDE FRAGMENTS; PROTEIN MULTIMERIZATION; REPTILIAN PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID;

BOTHROPS; BOTHROPS ALTERNATUS; VIPERIDAE;

EID: 77952545896     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxicon.2010.02.031     Document Type: Article

References (57)

1. Acosta de Perez O., Koscinczuk P., Teibler P., Sanchez Negrette M., Ruiz R., Maruñak S., Bogarín G. Actividades hemorrágica y edematizante y alteraciones histológicas en almohadilla plantar del ratón inducidas por venenos de serpientes de los géneros Bothrops y Crotalus de Argentina. Toxicon 1998, 36:1165-1172.
2. 2) from Bothrops jararacussu snake venom. Biochem. Pharmacol. 2002, 15:723-732.
3. 2, a structural review. Toxicon 1996, 34:827-841.
4. Calgarotto A.K., Damico D.C.S., Ponce-Soto L.A., Baldasso P.A., Da Silva S.L., Souza G.H.M.F., Eberlin M.N., Marangoni S. Biological and biochemical characterization of new basic phospholipase A2 BmTX-I isolated from Bothrops moojeni snake venom. Toxicon 2008, 51:1509-1519.
5. 2 by sequence analysis and site directed mutagenesis. Toxicon 2003, 42:869-883.
6. 2. Methods Enzymol. 1991, 197:75-79.
7. 2 from the venom of Bothrops insularis (jararaca ilhoa). Biochimie 2006, 88:1947-1959.
8. 2+-independent catalytic mechanism. Biochim. Biophys. Acta 2008, 1784:591-599.
9. 2 isoform isolated from Bothrops neuwiedii (yarará chica) venom with novel kinetic and chromatographic properties. Toxicon 1997, 35:1205-1215.
10. 2 from Bothrops erythromelas venom: isolation, cloning and characterization as potent anti-platelet and inductor of prostaglandin I2 release by endothelial cells. Biochem. Pharmacol. 2006, 72:377-384.
11. 2. J. Biol. Chem. 1994, 269:13057-13060.
12. 2 enzymes: structural diversity in lipid messenger metabolism. Struct. Fold. Des. 2000, 8:R15-R22.
13. Dufton M.J., Eaker D., Hider R.C. Conformational properties of phospholipases A. Secondary-structure prediction, circular dichroism and relative interface hydrophobicity. Eur. J. Biochem. 1983, 137:537-544.
14. 2. Possible role of NH2-terminal lysines in action on phospholipids of Escherichia coli. Biochemistry 1986, 29:4309-4314.
15. Gay C.C., Leiva L.C., Maruñak S., Teibler P., Acosta de Pérez O. Proteolytic, edematogenic and myotoxic activities of a hemorrhagic metalloproteinase isolated from Bothrops alternatus venom. Toxicon 2005, 46:546-554.
16. Gonçalves L.R.C., Mariano M. Local haemorrhage induced by Bothrops jararaca venom: relationship to neurogenic inflammation. Mediat. Inflamm. 2000, 9:101-107.
17. Gutiérrez J.M., Avila C., Rojas C., Cerdas L. An alternative in vitro method for testing the potency of the polyvalent antivenom produced in Costa Rica. Toxicon 1988, 26:411-413.
18. 2 myotoxins from Bothrops snake venoms. Toxicon 1995, 33:1405-1424.
19. 2 Enzymes: Estructure, Function and Mechanism 1997, 321-352. Wiley, Chichester. M.R. Kini (Ed.).
20. Gutiérrez J.M., Rucavado A. Snake venom metalloproteinases: their role in the pathogenesis of local tissue damage. Biochimie 2000, 82:841-845.
21. Gutiérrez J.M., Lomonte B. Efectos locales en el envenenamiento ofídico en América Latina. Animais Peconhentos no Brasil: Biologia, Clinica e Terapeutica dos Acidentes 2003, 310-323. Sarvier, Sao Paulo. J.L.C. Cardoso, F.O.S. Franca, F.H. Wen, C.M.S. Malaque, V. Haddad (Eds.).
22. 2: insights into the mechanisms of local and systemic myotoxicity. Toxicon 2003, 42:915-931.
23. 2 and the regeneration of skeletal muscles. Toxicon 2003, 42:933-945.
24. 2. Toxicon 1996, 34:1149-1155.
25. Kamiguti A.S., Hay C.R., Theakston R.D.G., Zuzel M. Insights into the mechanism of haemorrhage caused by snake venom metalloproteinases. Toxicon 1996, 34:627-642.
26. Kini M.R., Evans H.J. Structure-function relationships of phospholipases. The anticoagulant region of phospholipases A. J. Biol. Chem. 1987, 262:14402-14407.
27. 2 Enzymes: Structure, Function and Mechanism 1997, 1-28. Wiley, Chichester. R.M. Kini (Ed.).
28. 2 enzymes. Toxicon 2003, 42:827-840.
29. 2 enzymes from snake venoms. Toxicon 2005, 15:1147-1161.
30. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
31. 2 homologues: dissociation between enzymatic and biological activities. Eur. J. Pharmacol. 1998, 343:257-263.
32. 2. Toxicon 2000, 38:199-208.
33. 2 activity by a colorimetric assay using a pH indicator. Toxicon 1987, 25:1181-1188.
34. 2 myotoxins from crotalid snake venoms and their structural determinants of myotoxic action. Toxicon 2003, 42:885-901.
35. 2 from Bothrops jararacussu snake venom. Biocell 2007, 31:355-364.
36. Murakami M.T., Michelan-Duarte S., Cintra A.C., Arni R.K. Crystallization and high-resolution X-ray diffraction data collection of an Asp49 PLA2 from Bothrops jararacussu venom both in the presence and absence of Ca2+ ions. Biochim. Biophys. Acta 2004, 1703:79-81.
37. 2 from Bothrops alternatus (Víbora de la Cruz) venom. Toxicon 1988, 26:1137-1144.
38. 2 to the lethal potency of Bothrops alternatus (víbora de la cruz) venom. Toxicon 1986, 24:807-817.
39. 2 from Bothrops alternatus (víbora de la cruz) venom. Purification and some characteristic properties. Toxicon 1986, 24:259-272.
40. 2 from Agkistrodon piscivorus piscivorus snake venom: synthetic C-terminal peptides from Lys49, but not from Asp49 myotoxins, exert membrane-damaging activities. Toxicon 2001, 39:1587-1594.
41. Ownby C.L. Structure, function and biophysical aspects of the myotoxins from snake venoms. J. Toxicol. Toxin Rev. 1998, 17:1003-1009.
42. 2 proteins. Toxicon 1999, 37:411-445.
43. 2 from Crotalus durissus collilineatus venom. J. Protein Chem. 2002, 21,131-21,136.
44. 2 D49 from Bothrops jararacussu snake venom and neurotoxic characterization using in vitro neuromuscular preparation. Protein J. 2006, 25:147-155.
45. 2 homologue isolated from the venom of the snake Bothrops alternatus. BBA 2007, 1770:585-593.
46. Rosenfeld G. Symptomatology, pathology and treatment of snake bites in South America. Venomous Animals and Their Venoms 1971, 345-384. Academic Press, New York. W. Bucherl, E.E. Buckley (Eds.).
47. 2 superfamily and its group numbering system. BBA 2006, 1761:1246-1259.
48. 2, from the venom of the snake Bothrops jararaca: purification, primary structure analysis, and its characterization as a platelet-aggregation-inhibiting factor1. Arch. Biochem. Biophys. 1999, 367:26-32.
49. 2 enzymes: classification end characterization. Biochim. Biophys. Acta 2000, 1488:1-19.
50. 2 from snake venoms: effects on catalytic and pharmacological properties. Toxicon 2003, 42:855-868.
51. 2 myotoxins from Bothrops snake venoms: structure-function relationship. Curr. Org. Chem. 2004, 8:1677-1690.
52. Sperman-Karber Progress in the Characterization of Venoms and Standarization of Antivenoms 1981, World Health Organization, Geneva, 23-24 pp.
53. 2. Toxicon 2003, 42:947-962.
54. 2. Biochimie 2000, 82:815-821.
55. 2. Eur. J. Biochem. 1980, 112:25-32.
56. Vidal J.C., Stoppani A.O.M. Isolation and purification of two phospholipases A from Bothrops venoms. Arch. Biochem. Biophys. 1971, 145:543-556.
57. Yamakawa M., Nosaki M., Hokama Z. Fractionation of sakishima-hyabu (Trimererusus elegans) venom, and lethal, hemorrhagic and edema forming activities of the fractions. Animal, Plant, and Microbial Toxins 1976, vol. 1. Plenum Press, New York,. A. Ohsaka, K. Hayashi, Y. Sawai (Eds.).