Identification of the myotoxic site of the Lys49 phospholipase A2 from Agkistrodon piscivorus piscivorus snake venom: Synthetic C-terminal peptides from Lys49, but not from Asp49 myotoxins, exert membrane-damaging activities

Toxicon

Volumn 39, Issue 10, 2001, Pages 1587-1594

  Núez, Carlos E ^a   Angulo, Yamileth ^a   Lomonte, Bruno ^a  

^a UNIVERSIDAD DE COSTA RICA   (Costa Rica)

Author keywords

Heparin; Myotoxin; Phospholipase A2; Snake venom; Synthetic peptides

Indexed keywords

ASPARTIC ACID; CATION; LYSINE; PEPTIDE; PHOSPHOLIPASE A2; SNAKE VENOM; TOXIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CYTOLYSIS; CYTOTOXICITY; EDEMA; ENZYME ANALYSIS; MALE; MEMBRANE DAMAGE; MOUSE; MUSCLE; MUSCLE CELL; MUSCLE NECROSIS; NONHUMAN; PRIORITY JOURNAL; SKELETAL MUSCLE; SNAKE; TOXICITY; TOXIN ANALYSIS;

AGKISTRODON; AMINO ACID SEQUENCE; ANIMALS; ANTICOAGULANTS; ASPARAGINE; BOTHROPS; CELL LINE; CELL SURVIVAL; CELLS, CULTURED; CREATINE KINASE; CROTALID VENOMS; EDEMA; HEPARIN; INJECTIONS, INTRAMUSCULAR; INJECTIONS, SUBCUTANEOUS; L-LACTATE DEHYDROGENASE; LYSINE; MEMBRANES; MICE; MUSCLE, SKELETAL; NECROSIS; PHOSPHOLIPASES A; PROTEIN BINDING; PROTEIN ISOFORMS; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 0034899916     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0041-0101(01)00141-6     Document Type: Article

References (46)

1. 2 - A structural review
2. Structure of a calcium-independent phospholipase-like myotoxic protein from Bothrops asper venom
3. 2 from Bothrops asper snake venom
4. Inhibition of the myotoxic action of Bothrops asper myotoxin II in mice by immunization with its synthetic peptide 115-129
5. Bothropstoxin-I: Amino acid sequence and function
6. 2 superfamily of signal transduction enzymes
7. 2, anticoagulant and myotoxic activities
8. Molecular events in the myotoxic action of phospholipases
9. 2
10. 2: More than electrostatics and a major role for tryptophan
11. 2 myotoxins from Bothrops snake venoms
12. 2 myotoxins from Bothrops snake venoms
13. 2 from the venom of the cottonmouth snake at 2.0-Å resolution
14. The amino acid region 115-129 of ammodytoxins plays an important role in neurotoxicity
15. 2 into the porcine pancreatic isozyme dramatically changes the interfacial kinetics
16. 2 gene
17. The amino acid sequence of a myotoxic phospholipase from the venom of Bothrops asper
18. 2 enzymes
19. 2 homologue from Vipera ammodytes venom
20. A new muscle damaging toxin, myotoxin II, from the venom of the snake Bothrops asper (terciopelo)
21. 2 from Bothrops asper snake venom by glycosaminoglycans of the heparin/heparan sulfate family
22. 2 from Bothrops asper snake venom. Identification of a heparin-binding and cytolytic toxin region by the use of synthetic peptides and molecular modeling
23. The dynamics of local tissue damage induced by Bothrops asper snake venom and myotoxin II on the mouse cremaster muscle: An intravital and electron microscopic study
24. 2 expresses enhanced membrane-damaging activities and reproduces its in vivo myotoxic effect
25. 2 on mouse endothelial (tEnd) and skeletal muscle (C2C12) cells in vitro
26. 2
27. 2 with lysine in place of aspartate 49
28. 2
29. 2 from Bothrops asper snake venom: Synthetic Lys49 myotoxin II-(115-129)-peptide identifies its bactericidal region
30. 2 activity
31. 2 neurotoxins
32. 2 from bovine pancreas and Crotalus atrox venom
33. Phospholipases
34. Calcium ion independent membrane leakage induced by phospholipase-like myotoxins
35. 2: Structure and catalytic properties
36. 2 from the venom of Agkistrodon piscivorus piscivorus
37. 2 myotoxin from Agkistrodon contortrix laticinctus snake venom
38. 2
39. A rapid procedure for the isolation of the Lys-49 myotoxin II from Bothrops moojeni (caissaca) venom: Biochemical characterization, crystallization, myotoxic and edematogenic activity
40. 2 homologue from Bothrops moojeni (caissaca) snake venom
41. 2 homologue from Bothrops neuwiedi pauloensis venom
42. Amino acid sequence of piratoxin-I, a myotoxin from Bothrops pirajai snake venom and its biological activity after alkylation with p-bromophenacyl bromide
43. 2: Clues towards identification of residues involved in a novel mechanism of membrane damage and in myotoxicity
44. Fractionation of Sakishima-habu (Trimeresurus elegans) venom and lethal, hemorrhagic and edema-forming activities of the fractions
45. 2 with low activity, from the venom of Trimeresurus flavoviridis (Habu snake)
46. 2 on a membrane surface