Characterization of RNase HII substrate recognition using RNase HII-argonaute chimaeric enzymes from Pyrococcus furiosus

Biochemical Journal

Volumn 426, Issue 3, 2010, Pages 337-344

  Kitamura, Sayaka ^a   Fujishima, Kosuke ^a   Sato, Asako ^a   Tsuchiya, Daisuke ^a   Tomita, Masaru ^a   Kanai, Akio ^a  

^a KEIO UNIVERSITY   (Japan)

Author keywords

Archaeon; Argonaute; Double stranded RNA (dsRNA); Ribonuclease H; RNA DNA duplex; Site directed mutagenesis

Indexed keywords

ARCHAEON; ARGONAUTE; DNA DUPLEXES; DOUBLE-STRANDED RNA (DSRNA); SITE DIRECTED MUTAGENESIS;

AMINO ACIDS; CATALYSTS; DNA; ENZYMES; GENES; LIGHT MEASUREMENT; MANGANESE; MANGANESE COMPOUNDS; MODEL STRUCTURES; MUTAGENESIS; ORGANIC ACIDS; SUBSTRATES; THREE DIMENSIONAL;

RNA;

ARGONAUTE PROTEIN; DOUBLE STRANDED RNA; RIBONUCLEASE H; SMALL INTERFERING RNA; ARCHAEAL PROTEIN; ARGININE; ASPARTIC ACID; HYBRID PROTEIN; MANGANESE; PHENYLALANINE; RIBONUCLEASE; RIBONUCLEASE HII; RNA BINDING PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHIMERA; DNA RNA HYBRIDIZATION; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; PYROCOCCUS FURIOSUS; PYROCOCCUS HORIKOSHII; RNA RNA HYBRIDIZATION; WILD TYPE; BINDING SITE; CATALYSIS; CHEMICAL STRUCTURE; CHEMISTRY; DRUG EFFECT; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; SEQUENCE HOMOLOGY;

ARCHAEA; EUKARYOTA; PROKARYOTA; PYROCOCCUS FURIOSUS; PYROCOCCUS HORIKOSHII;

AMINO ACID SEQUENCE; ARCHAEAL PROTEINS; ARGININE; ASPARTIC ACID; BASE SEQUENCE; BINDING SITES; CATALYSIS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDORIBONUCLEASES; KINETICS; MANGANESE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PHENYLALANINE; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; PYROCOCCUS FURIOSUS; RECOMBINANT FUSION PROTEINS; RIBONUCLEASE H; RNA, DOUBLE-STRANDED; RNA-BINDING PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

EID: 77951887861     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20091553     Document Type: Article

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