Autonomous induction of recombinant proteins by minimally rewiring native quorum sensing regulon of E. coli

Metabolic Engineering

Volumn 12, Issue 3, 2010, Pages 291-297

  Tsao, Chen Yu ^a,b   Hooshangi, Sara ^a   Wu, Hsuan Chen ^a,b   Valdes, James J ^c   Bentley, William E ^a,b  

^a UNIVERSITY OF MARYLAND   (United States)

^b UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

^c EDGEWOOD CHEMICAL BIOLOGICAL CENTER   (United States)

Author keywords

AI 2; Metabolic burden; Metabolic engineering; Quorum sensing; Recombinant protein expression; Systems biology

Indexed keywords

AUTOINDUCER-2; AUTOINDUCERS; AUTONOMOUS INDUCTION; CHLORAMPHENICOL ACETYL TRANSFERASE; E. COLI; GALACTOSIDASES; GREEN FLUORESCENT PROTEIN; HIGH LEVEL EXPRESSION; INDIVIDUAL CELLS; MEDIUM EXCHANGE; METABOLIC ENGINEERING; METABOLIC ENGINEERING APPROACH; METABOLIC STATE; NEW PROCESS; OPTICAL DENSITY MEASUREMENTS; OVER-EXPRESSION; QUORUM-SENSING; RECOMBINANT PROTEIN; RECOMBINANT PROTEIN EXPRESSION; RECOMBINANT PROTEIN PRODUCTIONS; REGULATORY FUNCTIONS;

BACTERIOPHAGES; DENSITY MEASUREMENT (OPTICAL); ESCHERICHIA COLI; METABOLISM; SIGNAL TRANSDUCTION;

PROTEINS;

AUTOINDUCER 2; BETA GALACTOSIDASE; CHLORAMPHENICOL ACETYLTRANSFERASE; GREEN FLUORESCENT PROTEIN; INORGANIC COMPOUND; RECOMBINANT PROTEIN; SIGNAL PEPTIDE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; METABOLIC ENGINEERING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUORUM SENSING; REGULON; SIGNAL TRANSDUCTION;

BETA-GALACTOSIDASE; ESCHERICHIA COLI; HOMOSERINE; LACTONES; QUORUM SENSING; RECOMBINANT PROTEINS; REGULON; SIGNAL TRANSDUCTION;

BACTERIA (MICROORGANISMS);

EID: 77950858848     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2010.01.002     Document Type: Article

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