Heterologous expression of leader-less pga gene in Pichia pastoris: Intracellular production of prokaryotic enzyme

BMC Biotechnology

Volumn 10, Issue , 2010, Pages

  Marešová, Helena ^a   Marková, Zdena ^a   Valešová, Renáta ^a   Sklenář, Jan ^a   Kyslík, Pavel ^a  

^a INSTITUTE OF MICROBIOLOGY   (Czech Republic)

Author keywords

[No Author keywords available]

Indexed keywords

AMOXICILLIN; AUTOCATALYTIC; BACTERIAL EXPRESSION SYSTEMS; BACTERIAL PROTEIN; CEPHALEXIN; COMPLEX PROCESSING; CYTOPLASMIC MEMBRANE; CYTOSOLIC EXPRESSION; CYTOSOLS; FED-BATCH CULTURES; HETEROLOGOUS EXPRESSION; HYDROLYTIC ACTIVITIES; INTRACELLULAR PRODUCTION; OPTIMAL CONDITIONS; P. PASTORIS; PENICILLIN G; PENICILLIN G ACYLASE; PERIPLASM; PERIPLASMIC ENZYMES; PICHIA PASTORIS; PROKARYOTIC ENZYMES; PURIFIED ENZYME; SIGNAL SEQUENCE; TRANSIENT PHASIS; WILD TYPES; YEAST PRODUCTION;

BACTERIOLOGY; BATCH CELL CULTURE; CELL MEMBRANES; CYTOLOGY; DRUG PRODUCTS; ENCODING (SYMBOLS); ESCHERICHIA COLI; GENE ENCODING; GENE EXPRESSION; PURIFICATION; SEWAGE TREATMENT PLANTS; YEAST;

ENZYME ACTIVITY;

ACETANILIDE; BACTERIAL PROTEIN; CEFALEXIN; CELL ENZYME; PENICILLIN AMIDASE; PENICILLIN G; ESCHERICHIA COLI PROTEIN;

ARTICLE; BIOREACTOR; CARBOXY TERMINAL SEQUENCE; CELL DENSITY; CONTROLLED STUDY; CYTOSOL; ENZYME PURIFICATION; ENZYME SUBUNIT; ENZYME SYNTHESIS; FED BATCH CULTURE; FERMENTATION; HETEROLOGOUS EXPRESSION; HOST; HYDROLYSIS; MOLECULAR CLONING; NONHUMAN; PICHIA PASTORIS; PROKARYOTE; PROMOTER REGION; PROTEIN FUNCTION; VOLUMETRY; WILD TYPE; YEAST; BIOSYNTHESIS; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; MICROBIOLOGY; PICHIA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PICHIA; PICHIA PASTORIS; PROKARYOTA;

BIOREACTORS; CLONING, MOLECULAR; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FERMENTATION; INDUSTRIAL MICROBIOLOGY; PENICILLIN AMIDASE; PICHIA; PROMOTER REGIONS, GENETIC; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SUBSTRATE SPECIFICITY;

EID: 77950607349     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-10-7     Document Type: Article

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