Cloning, overexpression, and characterization of a novel thermostable penicillin G acylase from Achromobacter xylosoxidans: Probing the molecular basis for its high thermostability

Applied and Environmental Microbiology

Volumn 70, Issue 5, 2004, Pages 2764-2770

  Cai, Gang ^a   Zhu, Songcheng ^a   Yang, Sheng ^a   Zhao, Guoping ^a   Jiang, Weihong ^a  

^a INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CLONING; ESCHERICHIA COLI; GENES; MOLECULAR STRUCTURE; THERMODYNAMIC STABILITY;

HOMOLOGY; NUCLEOTIDES;

ENZYMES;

AMIDASE; AMINO ACID; ARGININE; NUCLEOTIDE; PENICILLIN G; PROLINE;

MICROBIOLOGY;

ACHROMOBACTER; ACHROMOBACTER XYLOSOXIDANS; ALCALIGENES FAECALIS; AMINO ACID COMPOSITION; AMINO ACID SEQUENCE; ARTICLE; ENTEROBACTERIACEAE; ENZYME ANALYSIS; ENZYME INACTIVATION; ESCHERICHIA COLI; KLUYVERA CRYOCRESCENS; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PROTEIN EXPRESSION; PROVIDENCIA RETTGERI; TEMPERATURE; THERMOSTABILITY;

ACHROMOBACTER DENITRIFICANS; AMINO ACID SEQUENCE; CLONING, MOLECULAR; ENZYME STABILITY; ESCHERICHIA COLI; HEAT; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; PENICILLIN AMIDASE; PHYLOGENY; SEQUENCE ANALYSIS, DNA;

ACHROMOBACTER; ACHROMOBACTER ALCALIGENES; ACHROMOBACTER XYLOSOXIDANS; ALCALIGENES; ALCALIGENES FAECALIS; ENTEROBACTERIACEAE; ESCHERICHIA COLI; KLUYVERA CRYOCRESCENS; NEGIBACTERIA; PROKARYOTA; PROVIDENCIA RETTGERI;

EID: 2442653152     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.5.2764-2770.2004     Document Type: Article

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