High-level production and covalent immobilization of Providencia rettgeri penicillin G acylase (PAC) from recombinant Pichia pastoris for the development of a novel and stable biocatalyst of industrial applicability

Biotechnology and Bioengineering

Volumn 93, Issue 2, 2006, Pages 344-354

  Senerovic, Lidija ^a   Stankovic, Nada ^a   Spizzo, Patrizia ^b   Basso, Alessandra ^b   Gardossi, Lucia ^b   Vasiljevic, Branka ^a   Ljubijankic, Goran ^a   Tisminetzky, Sergio ^c   Degrassi, Giuliano ^c  

^a INSTITUTE OF MOLECULAR GENETICS AND GENETIC ENGINEERING   (Serbia)

^b UNIVERSITY OF TRIESTE   (Italy)

^c INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Italy)

Author keywords

Covalent immobilization; Enzymatic synthesis of cephalosporins; Pichia pastoris; Providencia rettgeri; Recombinant penicillin G acylase

Indexed keywords

ANTIBIOTICS; BIOCATALYSTS; CELLS; FERMENTATION; GENES; METHANOL; PROTEINS;

COVALENT IMMOBILIZATION; ENZYMATIC SYNTHESIS OF CEPHALOSPORINS; PICHIA PASTORIS; PROVIDENCIA RETTGERI; RECOMBINANT PENICILLIN G ACYLASE;

ENZYME IMMOBILIZATION;

AMIDASE; BETA LACTAM ANTIBIOTIC; CEFALEXIN; METHANOL; PENICILLIN G ACYLASE; POLYMER; PROTEINASE; PROTEINASE A; RECOMBINANT ENZYME; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BIOCATALYST; CELL DENSITY; DRUG SYNTHESIS; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME INDUCTION; ENZYME STABILITY; FERMENTATION; GENE EXPRESSION; GENE INSERTION; HYDROLYSIS; KINETICS; NONHUMAN; PAC GENE; PEP4 GENE; PICHIA PASTORIS; PROVIDENCIA RETTGERI;

PICHIA PASTORIS; PROVIDENCIA RETTGERI;

EID: 33645557358     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.20728     Document Type: Article

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