Expression and purification of penicillin G acylase enzymes from four different micro-organisms, and a comparative evaluation of their synthesis/hydrolysis ratios for cephalexin

Protein Expression and Purification

Volumn 46, Issue 1, 2006, Pages 107-113

  Cheng, Tianfan ^a,b   Chen, Maolin ^a   Zheng, Huabao ^a,b   Wang, Jingang ^a   Yang, Sheng ^a   Jiang, Weihong ^a  

^a INSTITUTE OF PLANT PHYSIOLOGY AND ECOLOGY   (China)

^b ZHEJIANG UNIVERSITY   (China)

Author keywords

Cephalexin synthesis hydrolysis ratio; Immobilized metal chelate affinity chromatography; Penicillin G acylase; Protein purification

Indexed keywords

BACTERIAL PROTEIN; CEFALEXIN; PENICILLIN AMIDASE; PRIMER DNA; RECOMBINANT PROTEIN;

ALCALIGENES FAECALIS; ARTICLE; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; KINETICS; KLUYVERA; METABOLISM; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PROVIDENCIA; RESTRICTION MAPPING;

ALCALIGENES FAECALIS; BACTERIAL PROTEINS; BASE SEQUENCE; CEPHALEXIN; CLONING, MOLECULAR; DNA PRIMERS; ESCHERICHIA COLI; KINETICS; KLUYVERA; PENICILLIN AMIDASE; PROVIDENCIA; RECOMBINANT PROTEINS; RESTRICTION MAPPING; SUBSTRATE SPECIFICITY;

ALCALIGENES; ESCHERICHIA COLI; KLUYVERA CRYOCRESCENS; PROVIDENCIA RETTGERI;

EID: 32644472256     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.07.016     Document Type: Article

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