Improving the specific synthetic activity of a penicillin G acylase using DNA family shuffling

Acta Biochimica et Biophysica Sinica

Volumn 35, Issue 6, 2003, Pages 573-579

  Zhou, Zheng ^a   Zhang, Ai Hui ^a   Wang, Jing Ru ^b   Chen, Mao Lin ^b   Li, Ren Bao ^a   Yang, Sheng ^b   Yuan, Zhong Yi ^a  

^a INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

^b INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

Author keywords

DNA family shuffling; Penicillin G acylase; S H ratio

Indexed keywords

AMIDASE; PENICILLIN G;

ALPHA CHAIN; ARTICLE; BACTERIAL STRAIN; CHIMERA; ENZYME ACTIVITY; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE FUSION; GENE IDENTIFICATION; GENE LIBRARY; GENETIC RECOMBINATION; HYDROLYSIS; KLUYVEROMYCES; MULTIGENE FAMILY; PROTEIN ASSEMBLY; PROTEIN DOMAIN; PROTEUS RETTGERI; PROVIDENTIA; SUBSTITUTION REACTION; WILD TYPE;

DNA SHUFFLING; DNA, BACTERIAL; ENZYME ACTIVATION; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, ENZYMOLOGIC; KLUYVERA; PENICILLIN AMIDASE; PLASMIDS; POINT MUTATION; PROVIDENCIA; RECOMBINANT FUSION PROTEINS; RECOMBINATION, GENETIC; SEQUENCE HOMOLOGY;

BACTERIA (MICROORGANISMS); CHIMAERIFORMES; ESCHERICHIA COLI; KLUYVERA CRYOCRESCENS; KLUYVEROMYCES; PROVIDENCIA; PROVIDENCIA RETTGERI;

EID: 0041384171     PISSN: 05829879     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

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