The 7-hydroxylation of dehydroepiandrosterone in rat brain

Steroids

Volumn 75, Issue 6, 2010, Pages 404-410

  Li, Aiqun ^a   Bigelow, James C ^a  

^a IDAHO STATE UNIVERSITY   (United States)

Author keywords

7 OH DHEA; 7 OH DHEA; Brain microsomes; Brain mitochondria; DHEA; Metabolism

Indexed keywords

PRASTERONE;

ANIMAL EXPERIMENT; ARTICLE; BRAIN; CONTROLLED STUDY; ENZYME KINETICS; ENZYME METABOLISM; FEMALE; HYDROXYLATION; INCUBATION TIME; MALE; MICROSOME; MITOCHONDRION; NONHUMAN; RAT; WESTERN BLOTTING;

ANIMALS; BRAIN; BRAIN CHEMISTRY; DEHYDROEPIANDROSTERONE; ESTRADIOL; FEMALE; HYDROXYLATION; MALE; MICROSOMES; MITOCHONDRIA; MOLECULAR STRUCTURE; RATS; RATS, SPRAGUE-DAWLEY; STEROID HYDROXYLASES;

RATTUS;

EID: 77950517275     PISSN: 0039128X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.steroids.2010.02.003     Document Type: Article

References (33)

1. Bologa L., Sharma L., and Roberts E. Dehydroepiandrosterone and its sulfated derivative reduce neuronal death and enhance astrocytic differentiation in brain cell cultures. J Neurosci Res 17 (1987) 225-234
2. Compagnone N.A., and Mellon S.H. Dehydroepiandrosterone: a potential signaling molecular for neocortical organization during development. Proc Natl Acad Sci USA 95 (1998) 4678-4683
3. Cardounel A., Regelson W., and Kalimi M. Dehydroepiandrosterone protects hippocampal neurons against neurotoxin-induced cell death: mechanism of action. Proc Soc Exp Biol Med 222 (1999) 145-149
4. Kimonides V.G., Khatibi N.H., Svendsen C.N., Sofroniew M.V., and Herbert J. Dehydroepiandrosterone (DHEA) and DHEA-sulfate (DHEAS) protect hippocampal neurons against excitatory amino acid-induced neurotoxicity. Proc Natl Acad Sci USA 95 4 (1998) 1852-1857
5. Marx C.E., Jarskog L.F., Lauder J.M., Gilmore J.H., Lieberman J.A., and Morrow A.L. Neurosteroid modulation of embryonic neuronal survival in vitro following anoxia. Brain Res 871 1 (2000) 104-112
6. Kurata K., Takebayashi M., Morinobu S., and Yamawaki S. Beta-estradiol, dehydroepiandrosterone, and dehydroepiandrosterone sulfate protect against N-methyl-d-aspartate-induced neurotoxicity in rat hippocampal neurons by different mechanisms. J Pharmacol Exp Ther 311 1 (2004) 237-245
7. Baulieu E.E., and Robel P. Dehydroepiandrosterone (DHEA) and dehydroepiandrosterone sulfate (DHEAS) as neuroactive neurosteroids. Proc Natl Acad Sci USA 95 (1998) 4089-4091
8. Morfin R., and Starka L. Neurosteroid 7-hydroxylation products in the brain. In: Biggio G., and Purdy R.H. (Eds). International review of neurobiology, vol. 46. Neurosteroids and brain function (2001) 79-95
9. Stapleton G., Steel M., Richardson M., Mason J.O., Rose K.A., Morris R.G., et al. A novel cytochrome P450 expressed primarily in brain. J Biol Chem 270 (1995) 29739-29745
10. Schwarz M., Lund E.G., Lathe R., Bjorkhem I., and Russell D.W. Identification and characterization of a mouse oxysterol 7alpha-hydroxylase cDNA. J Biol Chem 272 (1997) 23995-24001
11. Rose K.A., Stapleton G., Dott K., Kieny M.P., Best R., Schwarz M., et al. Cyp7b, a novel brain cytochrome P450, catalyzes the synthesis of neurosteroids 7α-hydroxy dehydroepiandrosterone and 7α-hydroxy pregnenolone. Proc Natl Acad Sci USA 94 (1997) 4925-4930
12. Doostzadeh J., and Morfin R. Studies of the enzyme complex responsible for pregnenolone and dehydroepiandrosterone 7α-hydroxylation in mouse tissues. Steroids 61 (1996) 613-620
13. Doostzadeh J., Cotillon A.C., and Morfin R. Dehydroepiandrosterone 7α- and 7β-hydroxylation in mouse brain microsomes: effects of cytochrome P450 inhibitors and structure-specific inhibitor by steroid hormones. J Neuroendocrinol 9 (1997) 923-928
14. Rose K., Allan A., Gauldie S., Stapleton G., Dobbie L., Dott K., et al. Neurosteroid hydroxylase CYP7B-vivid reporter activity in dentate gyrus of gene-targeted mice and abolition of a widespread pathway of steroid and oxysterol hydroxylation. J Biol Chem 276 (2001) 23937-23944
15. Akwa Y., Morfin R., Robel P., and Baulieu E.E. Neurosteroid metabolism-7α-hydroxylation of dehydroepiandrosterone and pregnenolone in rat brain microsomes. Biochem J 288 (1992) 959-964
16. Kim S., Chalbot S., Pompon D., Jo D., and Morfin R. The human cytochrome P4507B1: catalytic activity studies. J Steroid Biochem Mol Biol 92 (2004) 383-389
17. Steckelbroeck S., Watthias M., Lutjohann D., Makiola P., Nassen A., Hans V.H.J., et al. Characterization of the dehydroepiandrosterone (DHEA) metabolism via oxysterol 7α-hydroxylase and 17-ketosteroid reductase activity in the human brain. J Neurochem 83 (2002) 713-726
18. Weill-Engerer S., David J., Sazdovitch V., Liere P., Schumacher M., Delacourte A., et al. In vitro metabolism of dehydroepiandrosterone (DHEA) to 7α-hydroxy-DHEA and 5-androsterene-3β,17β-diol in specific regions of the aging brain from Alzheimer's and non-demented patients. Brain Res 969 (2003) 117-125
19. 14C]-labeled 7alpha- and 7beta-hydroxylated derivatives of dehydroepiandrosterone and epiandrosterone. Steroids 67 (2002) 1121-1127
20. Clark J.B., and Lai J.C. Glycolytic, tricarboxylic acid cycle and related enzymes in brain. In: Boulton A.A., and Baker G.B. (Eds). Neuromethods (1989), Humana Press 233-281
21. Li A, May M, Bigelow J. An LC/MS Method for the Quantitative Determination of 7α-OH DHEA and 7β-OH DHEA: an Application for the Study of the Metabolism of DHEA in Rat Brain, Biomed Chromatogr 2010, in press, doi:10.1002/bmc.1371.
22. Mellon S.H., and Vaudry H. Biosynthesis of neurosteroids and regulation of their synthesis. In: Biggio G., and Purdy R.H. (Eds). International review of neurobiology, vol. 46. Neurosteroids and brain function (2001) 33-78
23. Hedlund E., Gustafsson J.A., and Warner M. Cytochrome P450 in the brain: a review. Curr Drug Metab 2 (2001) 245-263
24. Strobel H.W., Thompson C.M., and Antonovic L. Cytochromes P450 in brain: function and significance. Curr Drug Metab 2 2 (2001) 199-214
25. Liu M., Alkayed H., and Alkayed N.J. Cytochrome P450 in neurological disease. Curr Drug Metab 5 (2004) 225-234
26. Miksys S., Rao Y., Sellers E.M., Kwan M., Mendis D., and Tyndale R.F. Regional and cellular distribution of CYP2D subfamily members in rat brain. Xenobiotica 30 (2000) 547-564
27. Miksys S., and Tyndale R.F. Drug-metabolizing cytochrome P450 in the brain. J Psychiatry Neurosci 27 (2002) 406-415
28. Iscan M., Reuhl K., Weiss B., and Maines M.D. Regional and subcellular distribution of cytochrome P-450-dependent drug metabolism in monkey brain: the olfactory bulb and the mitochondrial fraction have high levels of activity. Biochem Biophys Res Commun 169 (1990) 858-863
29. Weill-Engerer S., David J., Sazdovitch V., Liere P., Eychenne B., Pianos A., et al. Neurosteroid quantification in human brain regions: comparison between Alzheimer's and nondemented patients. J Clin Endocrinol Metab 87 (2002) 5138-5143
30. Marwah A., Marwah P., and Lardy H. Ergosteroids. VI. Metabolism of dehydroepiandrosterone by rat liver in vitro: a liquid chromatographic-mass spectrometric study. J Chromatogr B: Anal Technol Biomed Life Sci 767 (2002) 285-299
31. Muller C., Pompon D., Urban P., and Morfin R. Inter-conversion of 7alpha- and 7beta-hydroxy-dehydroepiandrosterone by the human 11beta-hydroxysteroid dehydrogenase type 1. J Steroid Biochem Mol Biol 99 (2006) 215-222
32. Chalbot S., and Morfin R. Dehydroepiandrosterone metabolites and their interactions in humans. Drug Metabol Drug Interact 22 (2006) 1-23
33. Hennebert O., Chalbot S., Alran S., and Morfin R. Dehydroepiandrosterone 7alpha-hydroxylation in human tissues: possible interference with type 1 11beta-hydroxysteroid dehydrogenase-mediated processes. J Steroid Biochem Mol Biol 104 3-5 (2007) 326-333