Structure and mechanism of a Na+-independent amino acid transporter

Science

Volumn 325, Issue 5943, 2009, Pages 1010-1014

  Shaffer, Paul L ^a   Goehring, April ^a   Shankaranarayanan, Aruna ^a   Gouaux, Eric ^a  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID TRANSPORTER;

AMINO ACID; APOPTOSIS; CRYSTAL STRUCTURE; FUNCTIONAL GROUP; FUNCTIONAL ROLE; GENE EXPRESSION; ION; MEMBRANE; RESOLUTION; SODIUM;

ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; HUMAN; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN STRUCTURE; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; AMINO ACID TRANSPORT SYSTEMS; AMINO ACIDS; ANTIPORTERS; APOPROTEINS; ARCHAEAL PROTEINS; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI PROTEINS; METHANOCOCCUS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; PROTONS; SODIUM; SUBSTRATE SPECIFICITY;

EID: 69249116947     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1176088     Document Type: Article

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31. We appreciate the beamtime and support of the staffs at beamlines 8.2.1, 8.2.2, and 5.0.2 at the Advanced Light Source. We thank Z. Zhang for initial screening efforts, J. Michel for cloning of the 7F11 gene, L. Vaskalis for assistance with preparation of figures, and C. Piscitelli for analysis of the AdiC structure. P.L.S. was supported by an individual NIH National Research Service Award postdoctoral fellowship. This work was supported by the New York Consortium for Membrane Protein Structure, a NIH-supported Protein Structure Initiative (E.G.). E.G. is an investigator with the Howard Hughes Medical Institute. Coordinates and structure factors of the apo ApcT, ApcT-7F11 Fab complex, and ApcT K158A-7F11 Fab complex have been deposited in the Protein DataBank with accession codes 3GIA, 3GI9, and 3GI8, respectively.