Lys842 in neuronal nitric-oxide synthase enables the autoinhibitory insert to antagonize calmodulin binding, increase FMN shielding, and suppress interflavin electron transfer

Journal of Biological Chemistry

Volumn 285, Issue 5, 2010, Pages 3064-3075

  Guan, Zhi Wen ^a   Haque, Mohammad Mahfuzul ^a   Wei, Chin Chuan ^b   Garcin, Elsa D ^c   Getzoff, Elizabeth D ^d   Stuehr, Dennis J ^a  

^a LERNER RESEARCH INSTITUTE   (United States)

^b SOUTHERN ILLINOIS UNIVERSITY EDWARDSVILLE   (United States)

^c UNIVERSITY OF MARYLAND BALTIMORE COUNTY   (United States)

^d SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM CONCENTRATION; CALMODULIN BINDING; CHARGED RESIDUES; CONFORMATIONAL EQUILIBRIUM; CYTOCHROME C; ELECTRON TRANSFER; FREE STATE; MUTANT ENZYMES; NADPH OXIDASE; NEURONAL NITRIC-OXIDE SYNTHASE (NNOS); NITRIC-OXIDE SYNTHASE; REDUCTION KINETICS; STOPPED FLOW; SUB-DOMAINS; SUBDOMAIN; WILD TYPES;

CALCIUM; CALCIUM ALLOYS; CALMODULIN; ELECTRON TRANSITIONS; PORPHYRINS; SHIELDING;

CAMS;

ALANINE; CALCIUM; CALMODULIN; FLAVINE MONONUCLEOTIDE; FLAVOPROTEIN; GLUTAMINE; HEME; LYSINE; NEURONAL NITRIC OXIDE SYNTHASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE OXIDASE; CYTOCHROME REDUCTASE; NITRIC OXIDE SYNTHASE; RIBOFLAVIN DERIVATIVE;

AMINO ACID SUBSTITUTION; ARTICLE; AUTOINHIBITION; CATALYSIS; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CRYSTAL STRUCTURE; ELECTRON TRANSPORT; ENZYME ACTIVITY; EQUILIBRIUM CONSTANT; GENE INSERTION; INHIBITION KINETICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DETERMINATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; RAT; REDUCTION KINETICS; SPECTROPHOTOMETRY; STRUCTURE ANALYSIS; WILD TYPE; AMINO ACID SEQUENCE; ANIMAL; CATTLE; CHEMISTRY; ENZYMOLOGY; HUMAN; KINETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; NERVE CELL; PROTEIN TERTIARY STRUCTURE; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ANIMALS; CALMODULIN; CATTLE; CYTOCHROME REDUCTASES; FLAVINS; HUMANS; KINETICS; LYSINE; MOLECULAR SEQUENCE DATA; MUTATION; NADPH OXIDASE; NEURONS; NITRIC OXIDE SYNTHASE; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RATS; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 77449150569     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M109.000810     Document Type: Article

References (55)

1. Alderton, W. K., Cooper, C. E., and Knowles, R. G. (2001) Biochem. J. 357, 593-615
2. Roman, L. J., Martásek, P., and Masters, B. S. (2002) Chem. Rev. 102, 1179-1190
3. Stuehr, D. J., Wei, C. C., Wang, Z., and Hille, R. (2005) Dalton Trans. 21, 3427-3435
4. Bredt, D. S., Ferris, C. D., and Snyder, S. H. (1992) J. Biol. Chem. 267, 10976-10981
5. Hevel, J. M., and Marletta, M. A. (1992) Biochemistry 31, 7160-7165
6. Klatt, P., Schmidt, K., Uray, G., and Mayer, B. (1993) J. Biol. Chem. 268, 14781-14787
7. McMillan, K., Bredt, D. S., Hirsch, D. J., Snyder, S. H., Clark, J. E., and Masters, B. S. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 11141-11145
8. Schmidt, H. H., Smith, R. M., Nakane, M., and Murad, F. (1992) Biochemistry 31, 3243-3249
9. Stuehr, D. J., Cho, H. J., Kwon, N. S., Weise, M. F., and Nathan, C. F. (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 7773-7777
10. Stuehr, D. J., and Ikeda-Saito, M. (1992) J. Biol. Chem. 267, 20547-20550
11. Guan, Z. W., Kamatani, D., Kimura, S., and Iyanagi, T. (2003) J. Biol. Chem. 278, 30859-30868
12. Marletta, M. A., Hurshman, A. R., and Rusche, K. M. (1998) Curr. Opin. Chem. Biol. 2, 656-663
13. Miller, R. T., Martásek, P., Omura, T., and Siler Masters, B. S. (1999) Biochem. Biophys. Res. Commun. 265, 184-188
14. Stuehr, D. J., Santolini, J., Wang, Z. Q., Wei, C. C., and Adak, S. (2004) J. Biol. Chem. 279, 36167-36170
15. Bredt, D. S., and Snyder, S. H. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 682-685
16. Busse, R., and Mülsch, A. (1990) FEBS Lett. 265, 133-136
17. Cho, H. J., Xie, Q. W., Calaycay, J., Mumford, R. A., Swiderek, K. M., Lee, T. D., and Nathan, C. (1992) J. Exp. Med. 176, 599-604
18. Bredt, D. S., Hwang, P. M., Glatt, C. E., Lowenstein, C., Reed, R. R., and Snyder, S. H. (1991) Nature 351, 714-718
19. Roman, L. J., Martásek, P., Miller, R. T., Harris, D. E., de La Garza, M. A., Shea, T. M., Kim, J. J., and Masters, B. S. (2000) J. Biol. Chem. 275, 29225-29232
20. Bauer, P. M., Fulton, D., Boo, Y. C., Sorescu, G. P., Kemp, B. E., Jo, H., and Sessa, W. C. (2003) J. Biol. Chem. 278, 14841-14849
21. Dimmeler, S., Fleming, I., Fisslthaler, B., Hermann, C., Busse, R., and Zeiher, A. M. (1999) Nature 399, 601-605
22. Komeima, K., Hayashi, Y., Naito, Y., and Watanabe, Y. (2000) J. Biol. Chem. 275, 28139-28143
23. Michell, B. J., Harris, M. B., Chen, Z. P., Ju, H., Venema, V. J., Blackstone, M. A., Huang, W., Venema, R. C., and Kemp, B. E. (2002) J. Biol. Chem. 277, 42344-42351
24. Salerno, J. C., Harris, D. E., Irizarry, K., Patel, B., Morales, A. J., Smith, S. M., Martasek, P., Roman, L. J., Masters, B. S., Jones, C. L., Weissman, B. A., Lane, P., Liu, Q., and Gross, S. S. (1997) J. Biol. Chem. 272, 29769-29777
25. Daff, S., Sagami, I., and Shimizu, T. (1999) J. Biol. Chem. 274, 30589-30595
26. Montgomery, H. J., Romanov, V., and Guillemette, J. G. (2000) J. Biol. Chem. 275, 5052-5058
27. Nishida, C. R., and Ortiz de Montellano, P. R. (1999) J. Biol. Chem. 274, 14692-14698
28. Roman, L. J., and Masters, B. S. (2006) J. Biol. Chem. 281, 23111-23118
29. Chen, P. F., and Wu, K. K. (2000) J. Biol. Chem. 275, 13155-13163
30. Chen, P. F., and Wu, K. K. (2003) J. Biol. Chem. 278, 52392-52400
31. Guan, Z. W., and Iyanagi, T. (2003) Arch. Biochem. Biophys. 412, 65-76
32. Matsuda, H., and Iyanagi, T. (1999) Biochim. Biophys. Acta 1473, 345-355
33. Abu-Soud, H. M., and Stuehr, D. J. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 10769-10772
34. Panda, K., Ghosh, S., and Stuehr, D. J. (2001) J. Biol. Chem. 276, 23349-23356
35. Rozhkova, E. A., Fujimoto, N., Sagami, I., Daff, S. N., and Shimizu, T. (2002) J. Biol. Chem. 277, 16888-16894
36. Craig, D. H., Chapman, S. K., and Daff, S. (2002) J. Biol. Chem. 277, 33987-33994
37. Konas, D. W., Zhu, K., Sharma, M., Aulak, K. S., Brudvig, G. W., and Stuehr, D. J. (2004) J. Biol. Chem. 279, 35412-35425
38. Feng, C., Tollin, G., Holliday, M. A., Thomas, C., Salerno, J. C., Enemark, J. H., and Ghosh, D. K. (2006) Biochemistry 45, 6354-6362
39. Garcin, E. D., Bruns, C. M., Lloyd, S. J., Hosfield, D. J., Tiso, M., Gachhui, R., Stuehr, D. J., Tainer, J. A., and Getzoff, E. D. (2004) J. Biol. Chem. 279, 37918-37927
40. Ilagan, R. P., Tiso, M., Konas, D. W., Hemann, C., Durra, D., Hille, R., and Stuehr, D. J. (2008) J. Biol. Chem. 283, 19603-19615
41. Tiso, M., Konas, D. W., Panda, K., Garcin, E. D., Sharma, M., Getzoff, E. D., and Stuehr, D. J. (2005) J. Biol. Chem. 280, 39208-39219
42. Tiso, M., Tejero, J., Panda, K., Aulak, K. S., and Stuehr, D. J. (2007) Biochemistry 46, 14418-14428
43. Adak, S., Ghosh, S., Abu-Soud, H. M., and Stuehr, D. J. (1999) J. Biol. Chem. 274, 22313-22320
44. Adak, S., Aulak, K. S., and Stuehr, D. J. (2001) J. Biol. Chem. 276, 23246-23252
45. Haque, M. M., Panda, K., Tejero, J., Aulak, K. S., Fadlalla, M. A., Mustovich, A. T., and Stuehr, D. J. (2007) Proc. Natl. Acad. Sci. U.S.A. 104, 9254-9259
46. Haque, M. M., Fadlalla, M., Wang, Z. Q., Ray, S. S., Panda, K., and Stuehr, D. J. (2009) J. Biol. Chem. 284, 19237-19247
47. Gachhui, R., Abu-Soud, H. M., Ghosha, D. K., Presta, A., Blazing, M. A., Mayer, B., George, S. E., and Stuehr, D. J. (1998) J. Biol. Chem. 273, 5451-5454
48. Wu, C., Zhang, J., Abu-Soud, H., Ghosh, D. K., and Stuehr, D. J. (1996) Biochem. Biophys. Res. Commun. 222, 439-444
49. Adak, S., Santolini, J., Tikunova, S., Wang, Q., Johnson, J. D., and Stuehr, D. J. (2001) J. Biol. Chem. 276, 1244-1252
50. Adak, S., Sharma, M., Meade, A. L., and Stuehr, D. J. (2002) Proc. Natl. Acad. Sci. U.S.A. 99, 13516-13521
51. Knight, K., and Scrutton, N. S. (2002) Biochem. J. 367, 19-30
52. Garnaud, P. E., Koetsier, M., Ost, T. W., and Daff, S. (2004) Biochemistry 43, 11035-11044
53. Daff, S. (2004) Biochemistry 43, 3929-3932
54. Konas, D. W., Takaya, N., Sharma, M., and Stuehr, D. J. (2006) Biochemistry 45, 12596-12609
55. Jáchymová, M., Martásek, P., Panda, S., Roman, L. J., Panda, M., Shea, T. M., Ishimura, Y., Kim, J. J., and Masters, B. S. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 15833-15838