Establishment of cyanophycin biosynthesis in Pichia pastoris and optimization by use of engineered cyanophycin synthetases

Applied and Environmental Microbiology

Volumn 76, Issue 4, 2010, Pages 1062-1070

  Steinle, Anna ^a   Witthoff, Sabrina ^a   Krause, Jens P ^a   Steinbüchel, Alexander ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL OXIDASE 1 PROMOTER; C TERMINUS; CATALYTIC PROPERTIES; CULTIVATION PERIODS; CYANOBACTERIUM SYNECHOCYSTIS; CYANOPHYCIN; EXPRESSION VECTORS; IN-CELL; L-ASPARTIC ACID; MEDIUM COMPOSITION; METHYLOTROPHIC YEASTS; MUTATED GENES; OPTIMAL TEMPERATURE; P. PASTORIS; PICHIA PASTORIS; POLYMER CONTENT; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS; SYNTHETASES; WILD TYPES;

AMINO ACIDS; BIOCHEMISTRY; CLONING; ENZYME ACTIVITY; ENZYMES; ESCHERICHIA COLI; FERMENTATION; OPTIMIZATION; ORGANIC ACIDS; PHOSPHATASES; SYNTHESIS (CHEMICAL); YEAST;

GENE EXPRESSION;

BACTERIAL PROTEIN; CYANOPHYCIN; CYANOPHYCIN SYNTHASE, BACTERIA; PEPTIDE SYNTHASE; RECOMBINANT DNA; RECOMBINANT PROTEIN; VEGETABLE PROTEIN;

BACTERIUM; ENZYME ACTIVITY; FERMENTATION; FUNGUS; GENE; GENE EXPRESSION; OPTIMIZATION;

ARTICLE; BACTERIAL GENE; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; FERMENTATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; KINETICS; METABOLISM; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PH; PICHIA; PROTEIN ENGINEERING; SITE DIRECTED MUTAGENESIS; SYNECHOCYSTIS; TEMPERATURE;

BACTERIAL PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; DNA, RECOMBINANT; ESCHERICHIA COLI; FERMENTATION; GENES, BACTERIAL; HYDROGEN-ION CONCENTRATION; KINETICS; MUTAGENESIS, SITE-DIRECTED; PEPTIDE SYNTHASES; PICHIA; PLANT PROTEINS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SYNECHOCYSTIS; TEMPERATURE;

ESCHERICHIA COLI; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE; SYNECHOCYSTIS SP.;

EID: 76649135811     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01659-09     Document Type: Article

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