Physiological conditions conducive to high cyanophycin content in biomass of Acinetobacter calcoaceticus Strain ADP1

Applied and Environmental Microbiology

Volumn 71, Issue 2, 2005, Pages 858-866

  Elbahloul, Yasser ^a   Krehenbrink, Martin ^a,b   Reichelt, Rudolf ^a   Steinbüchel, Alexander ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

^b JOHN INNES CENTRE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ACIDS; BIOSYNTHESIS; CARBON; CELLS; GENES; INORGANIC COMPOUNDS; MINERALS; MOLECULAR WEIGHT; MUTAGENESIS; NITROGEN; ORGANIC COMPOUNDS; OXYGEN; PH EFFECTS; SODIUM COMPOUNDS; SULFUR COMPOUNDS;

ARGININE (ARG); CYANOPHYCIN; INCUBATION TEMPERATURES; PHYCHOLOGICAL CONDITIONS;

BACTERIA;

AMMONIUM SULFATE; ARGININE; ASPARTIC ACID; BACTERIAL ENZYME; BACTERIAL PROTEIN; CARBON; CHLORAMPHENICOL; CYANOPHYCIN; GLUTAMATE SODIUM; LYSINE; NITROGEN; PHOSPHATE; POLYPEPTIDE; SYNTHETASE; UNCLASSIFIED DRUG;

BACTERIUM;

ACINETOBACTER CALCOACETICUS; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BIOMASS PRODUCTION; CARBON SOURCE; CELL SURFACE; CONTROLLED STUDY; GENE MUTATION; INCUBATION TEMPERATURE; INORGANIC NUTRIENT; MICROBIAL BIOMASS; MOLECULAR WEIGHT; NONHUMAN; OXYGEN SUPPLY; PH; PROTEIN ANALYSIS; PROTEIN SECRETION; PROTEIN SYNTHESIS; TRANSMISSION ELECTRON MICROSCOPY;

ACINETOBACTER CALCOACETICUS; BACTERIAL PROTEINS; BIOMASS; CHLORAMPHENICOL; CULTURE MEDIA; HYDROGEN-ION CONCENTRATION; MUTATION; OXYGEN; PEPTIDE HYDROLASES; PEPTIDE SYNTHASES; PLANT PROTEINS; TEMPERATURE;

ACINETOBACTER CALCOACETICUS; ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); UNCULTURED ACTINOMYCETE;

EID: 13544249631     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.2.858-866.2005     Document Type: Article

References (49)

1. Aboulmagd, E., F. B. Oppermann-Sanio, and A. Steinbüchel. 2000. Molecular characterization of the cyanophycin synthetase from Synechocystis sp. strain PCC6308. Arch. Microbiol. 174:297-306.
2. Aboulmagd, E., F. B. Oppermann-Sanio, and A. Steinbüchel. 2001. Purification of Synechocystis sp. strain PCC 6308 cyanophycin synthetase and its characterization with respect to substrate and primer specificity. Appl. Environ. Microbiol. 67:2176-2182.
3. Aboulmagd, E., I. Voss, F. B. Oppermann-Sanio, and A. Steinbüchel. 2001. Heterologous expression of cyanophycin synthetase and cyanophycin synthesis in the industrial relevant bacteria Corynebacterium glutamicum and Ralstonia eutropha and in Pseudomonas putida. Biomacromolecules 2:1338-1342.
4. Aldor, I., and J. D. Keasling. 2001. Metabolic engineering of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) composition in recombinant Salmonella enterica serovar Typhimurium. Biotechnol. Bioeng. 76:108-114.
5. Alford, D. D., A. P. Wheeler, and C. A. Pettigrew. 1994. Biodegradation of thermally synthesized polyaspartate. J. Environ. Polym. Degrad. 2:225-236.
6. Alvarez, H. M., O. H. Pucci, and A. Steinbüchel. 1997. Lipid storage compounds in marine bacteria. Appl. Microbiol. Biotechnol. 47:132-139.
7. Arnold, M. 1968. Histochemie. Einführung in die Grundlagen und Prinzipien der Methoden. Springer-Verlag, Berlin, Germany.
8. Berg, H., K. Ziegler, K. Piotukh, K. Baier, W. Lockau, and R. Volkmer-Engert. 2000. Biosynthesis of the cyanobacterial reserve polymer multi-L-arginylpoly-L-aspartic acid (cyanophycin). Mechanism of the cyanophycin synthetase reaction studied with synthetic primers. Eur. J. Biochem. 267:5561-5570.
9. Birnboim, H. C., and J. Doly. 1979. A rapid alkaline procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513-1523.
10. Borzi, A. 1887. Le comunicazioni intracellulari delle Nostochinee. Malpighia 1:28-74.
11. Bradford, M. M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
12. Carr, N. G. 1988. Nitrogen reserves and dynamics reservoirs in cyanobacteria, p. 13-21. In L. J. Rogers and J. R. Gallon (ed.), Biochemistry of the algae and cyanobacteria. Annual Proceedings of the Phytochemical Society of Europe. Clarendon, Oxford, United Kingdom.
13. Colón-López, M. S., D. M. Sherman, and L. A. Sherman. 1997. Transcriptional and translational regulation of nitrogenase in light-dark- and continuous-light-grown cultures of the unicellular cyanobacterium Cyanothece sp. strain ATCC 51142. J. Bacteriol. 179:4319-4327.
14. Frey, K. M., F. B. Oppermann-Sanio, H. Schmidt, and A. Steinbüchel. 2002. Technical scale production of cyanophycin with recombinant strains of Escherichia coli. Appl. Environ. Microbiol. 68:3377-3384.
15. Gupta, M., and N. G. Carr. 1981. Enzyme activities related to cyanophycin metabolism in heterocysts and vegetative cells of Anabaena sp. J. Gen. Microbiol. 125:17-23.
16. Hai, T., F. B. Oppermann-Sanio, and A. Steinbüchel. 1999. Purification and characterization of cyanophycin and cyanophycin synthetase from the thermophilic Synechococcus sp. MA19. FEMS Microbiol. Lett. 181:229-236.
17. Hanahan, D. 1983. Studies on the transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580.
18. Howarth, R. W., and J. J. Cole. 1985. Molybdenum availability, nitrogen limitation, and phytoplankton growth in natural waters. Science 229:635-655.
19. Joentgen, W., T. Groth, A. Steinbüchel, T. Hai, and F. B. Oppermann. 1998. Polyaspartic acid homopolymers and copolymers, biotechnological production and use thereof. International patent application WO 98/39090.
20. Krehenbrink, M., F. B. Oppermann-Sanio, and A. Steinbüchel. 2002. Evaluation of non-cyanobacterial genome sequences for occurrence of genes encoding proteins homologous to cyanophycin synthetase and cloning of an active cyanophycin synthetase from Acinetobacter sp. strain DSM 587. Arch. Microbiol. 177:371-380.
21. Krehenbrink, M., and A. Steinbüchel. 2004. Partial purification and characterization of a non-cyanobacterial cyanophycin synthetase from Acinetobacter calcoaceticus strain ADP1 with regard to substrate specificity, substrate affinity and binding to cyanophycin. Microbiology 150:2599-2608.
22. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
23. Lang, N. J., R. D. Simon, and C. P. Wolk. 1972. Correspondence of cyanophycin granules with structured granules in Anabaena cylindrica. Arch. Microbiol. 83:313-320.
24. Li, H., D. M. Sherman, S. Bao, and L. A. Sherman. 2001. Pattern of cyanophycin accumulation in nitrogen-fixing and non-nitrogen-fixing cyanobacteria. Arch. Microbiol. 176:9-18.
25. Mackerras, A. H., N. M. de Chazal, and G. D. Smith. 1990. Transient accumulation of cyanophycin in Anabaena cylindrical and Synechocystis 6308. J. Gen. Microbiol. 136:2057-2065.
26. Merritt, M. V., S. S. Sid, L. Mesh, and M. M. Allen. 1994. Variations in the amino acid composition of cyanophycin in the cyanobacterium Synechocystis sp. PCC6308 as a function of growth conditions. Arch. Microbiol. 162:158-166.
27. Obst, M., F. B. Oppermann-Sanio, and A. Steinbüchel. 2002. Isolation of cyanophycin degrading bacteria, cloning and characterization of an extracellular cyanophycinase gene (cphE) from Pseudomonas anguilliseptica strain BI - the cphE gene from P. anguilliseptica BI encodes a cyanophycin-hydrolyzing enzyme. J. Biol. Chem. 277:25096-25105.
28. Obst, M., A. Sallam, H. Luftmann, and A. Steinbüchel. 2003. Isolation and characterization of Gram-positive cyanophycin-degrading bacteria - kinetic studies on cyanophycin depolymerase activity in aerobic bacteria. Biomacromolecules 5:153-161.
29. Oppermann-Sanio, F. B., T. Hai, E. Aboulmagd, F. F. Hezayen, S. Jossek, and A. Steinbüchel. 1999. Biochemistry of microbial polyamide metabolism, p. 185-193. In A. Steinbüchel (ed.), Biochemical principles and mechanisms of biosynthesis and biodegradation of polymers. Wiley-VCH, Weinheim, Germany.
30. Palmen, R., B. Vosman, P. Buijsman, C. K. Breek, and K. J. Hellingwerf. 1993. Physiological characterization of natural transformation in Acinetobacter calcoaceticus. J. Gen. Microbiol. 139:295-305.
31. Picossi, S., A. Valladares, E. Flores, and A. Herrero. 2004. Nitrogen-regulated genes for the metabolism of cyanophycin, a bacterial nitrogen reserve polymer. J. Biol. Chem. 279:11582-11592.
32. Quintero, M. J., A. M. Muro-Pastor, A. Herrero, and E. Flores. 2000. Arginine catabolism in the cyanobacterium Synechocystis sp. strain PCC 6803 involves the urea cycle and argininase pathway. J. Bacteriol. 182:1008-1015.
33. Rao, N. R., M. A. Richardson, and S. Kuhstoss. 1987. Cosmid shuttle vectors for cloning and analysis of Streptomyces DNA. Methods Enzymol. 153:166-198.
34. Richter, R., M. Hejazi, R. Kraft, K. Ziegler, and W. Lockau. 1999. Cyanophycinase, a peptidase degrading the cyanobacterial reserve material multi-L-arginyl-poly-L-aspartic acid (cyanophycin). Molecular cloning of the gene of Synechocystis sp. PCC6803, expression in Escherichia coli, and biochemical characterization of the purified enzyme. Eur. J. Biochem. 263:163-169.
35. Roweton, S., S. J. Huang, and G. Swift. 1997. Poly(aspartic acid): synthesis, biodegradation, and current applications. J. Environ. Polym. Degrad. 5:175-181.
36. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory. Cold Spring Harbor, N.Y.
37. Schlegel, H. G. 1992. Allgemeine Mikrobiologie, 2nd ed. Thieme Verlag, Stuttgart, Germany.
38. Schmidt, K., S. Liaanen-Jensen, and H. G. Schlegel. 1963. Die Carotinoide der Thiorhodaceae. Arch. Microbiol. 46:117-126.
39. Schwamborn, M. 1998. Chemical synthesis of polyaspartates: a biodegradable alternative to currently used polycarboxylate homo- and copolymers. Polym. Degrad. Stabil. 59:39-45.
40. 2-fixing cultures of Anabaena sp. PCC 7120 (Cyanobacteria). J. Phycol. 36:932-941.
41. Simon, R. D. 1971. Cyanophycin granules from the blue-green alga Anabaena cylindrica: a reserve material consisting of copolymer of aspartic acid and arginine. Proc. Natl. Acad. Sci. USA 68:265-267.
42. Simon, R. D. 1973. The effect of chloramphenicol on the production of cyanophycin granule polypeptide in the blue-green alga Anabaena cylindrica. Arch. Microbiol. 92:115-122.
43. Simon, R. D. 1976. The biosynthesis of multi-L-arginyl-poly(L-aspartic acid) in the filamentous cyanobacterium Anabaena cylindrica. Biochim. Biophys. Acta 422:407-418.
44. Simon, R. D., and P. Weathers. 1976. Determination of the structure of the novel polypeptide containing aspartic acid and arginine which is found in cyanobacteria. Biochim. Biophys. Acta 420:165-176.
45. Simon, R. D. 1987. Inclusion bodies in the cyanobacteria: cyanophycin, polyphosphate, polyhedral bodies, p. 199-225. In P. Fay and C. van Baalen (ed.), The cyanobacteria. Elsevier, Amsterdam, The Netherlands.
46. Spurr, A. R. 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26:31-43.
47. Weber, K., and M. Osborn. 1969. The reliability of molecular weight determination by dodecylsulfate-polyacrylamide gel electrophoresis. J. Biol. Chem. 244:4406-4412.
48. Ziegler, K., A. Diener, C. Herpin, R. Richter, R. Deutzmann, and W. Lockau. 1998. Molecular characterization of cyanophycin synthetase, the enzyme catalyzing the biosynthesis of the cyanobacterial reserve material multi-L-arginyl-poly-L-aspartate (cyanophycin). Eur. J. Biochem. 254:154-159.
49. Ziegler, K., R. Deutzmann, and W. Lockau. 2002. Cyanophycin synthetase-like enzymes of non-cyanobacterial eubacteria: characterization of the polymer produced by a recombinant synthetase of Desulfitobacterium hafniense. Z. Naturforsch. Sect. C 57:522-529.