Optimization of chemically defined medium for recombinant Pichia pastoris for biomass production

Bioresource Technology

Volumn 99, Issue 16, 2008, Pages 7906-7910

  Ghosalkar, A ^a   Sahai, V ^a   Srivastava, A ^b  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

^b DEPARTMENT OF ELECTRONICS AND COMPUTER ENGINEERING   (Singapore)

Author keywords

Chemostat; Glycerol; Pichia pastoris; Statistical optimization

Indexed keywords

AEROSPACE APPLICATIONS; BIOLOGICAL MATERIALS; BIOMASS; CARBON; CURVE FITTING; FORESTRY; GLYCEROL; METALS; NUMERICAL METHODS; NUTRITION; RENEWABLE ENERGY RESOURCES; STATISTICAL METHODS; TRACE ELEMENTS;

BIOMASS PRODUCTIONS; CULTURES (TRADITIONAL); FERMENTOR; MEDIUM COMPOSITION; OPTIMAL LEVELS; P. PASTORIS; PICHIA PASTORIS (PPPFK); RESPONSE SURFACE METHODOLOGY (RSA); SOLE CARBON SOURCE; TRACE METALS;

OPTIMIZATION;

GLYCEROL;

ACETATE; ALCOHOL; BIOMASS; CARBON; CHEMOSTAT; CULTIVATION; DILUTION; EXPERIMENTAL STUDY; FERMENTATION; GROWTH RATE; NUTRITIONAL REQUIREMENT; OPTIMIZATION; PROTEIN; REACTION KINETICS; RECOMBINATION; RESPIRATION; TRACE ELEMENT; VITAMIN; YEAST;

ARTICLE; BIOMASS; CHEMOSTAT; DILUTION; FUNGAL CELL; FUNGUS CULTURE; FUNGUS GROWTH; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PICHIA PASTORIS; PRIORITY JOURNAL; RESPONSE SURFACE METHOD; STEADY STATE;

AMMONIUM SULFATE; BIOMASS; BIOREACTORS; CARBON; CULTURE MEDIA; GLYCEROL; HYDROGEN-ION CONCENTRATION; INTERFERON ALFA-2B; KINETICS; MODELS, CHEMICAL; NITROGEN; PICHIA; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

BIOMASS; CARBON; ETHANOL; FORESTRY; GLYCEROL; NATURAL RESOURCES; NUTRITION; OPTIMIZATION; PROTEINS; STATISTICAL METHODS; TRACE ELEMENTS; VITAMINS;

PICHIA PASTORIS;

EID: 45449089448     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2008.01.059     Document Type: Article

References (12)

1. Aguilaniu H., Gustafsson L., Rigoulet M., and Nyström T. Protein oxidation in G0 cells of Saccharomyces cerevisiae depends on the state rather than rate of respiration and is enhanced in pos9 but not yap1 mutants. J. Biol. Chem. 276 (2001) 35396-35404
2. Box G.E.P., and Behnken D.W. Some new three level designs for the study of quantitative variables. Technometrics. 2 (1960) 455-475
3. Box G.E.P., and Hunter J.S. Multi-factorial designs for exploring response surfaces. Ann. Math. Stat. 28 (1957) 195-241
4. Cereghino G.P., Cereghino J.L., Ilgen C., and Cregg J.M. Production of recombinant proteins in fermentor cultures of the yeast Pichia pastoris. Curr. Opin. Biotechnol. 13 (2002) 329-332
5. Cos O., Ramon R., Montesinos J., and Valero F. Operational strategies, monitoring and control of heterologous protein production in the methylotropic yeast Pichia pastoris under different promoters. Microb. Cell Fact. 5 (2006) 17-37
6. D'Anjou M.C., and Daugulis A.J. Mixed-feed exponential feeding for fed-batch culture of recombinant methylotrophic yeast. Biotechnol. Lett. 22 (2000) 341-346
7. Francis F., Sabu A., Nampoothiri K.M., Ramachandran S., Ghosh S., Szakacs G., and Pandey A. Use of response surface methodology for optimizing process parameters for the production of α-amylase by Aspergillus oryzae. Biochem. Eng. J. 15 (2003) 107-115
8. Hoskisson P.A., and Hobbs G. Continuous culture-making a comeback. Microbiology 151 (2005) 3153-3159
9. Monod J. The growth of bacterial cultures. Annu. Rev. Microbiol. 3 (1949) 371-394
10. Pichia fermentation process guidelines. invitrogen Corp., (www.invitrogen.com).
11. Sola A., Maaheimo H., Ylonen K., Ferrer P., and Szyperski T. Amino acid biosynthesis and metabolic flux profiling of Pichia pastoris. Eur. J. Biochem. 271 (2004) 2462-2470
12. Stratton J., Chiruvolu V., and Meagher M.M. High cell-density fermentation. In: Higgins D.R., and Cregg J.M. (Eds). Methods in Molecular Biology vol. 103 (1998), Humana Press, Totowa, NJ 107-120