Biosynthesis of the cyanobacterial reserve polymer multi-L-arginyl-poly-L-aspartic acid (cyanophycin): Mechanism of the cyanophycin synthetase reaction studied with synthetic primers

European Journal of Biochemistry

Volumn 267, Issue 17, 2000, Pages 5561-5570

  Berg, Holger ^a   Ziegler, Karl ^a   Piotukh, Kirill ^a   Baier, Kerstin ^a   Lockau, Wolfgang ^a   Volkmer Engert, Rudolf ^b  

^a HUMBOLDT UNIVERSITY   (Germany)

^b CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

Cyanobacteria; Cyanophycin synthetase; Mechanism; Nonribosomal peptide biosynthesis

Indexed keywords

AMINO ACID; ASPARTIC ACID DERIVATIVE; CYANOPHYCIN; PEPTIDE SYNTHASE; POLYPEPTIDE; UNCLASSIFIED DRUG;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN SYNTHESIS; REACTION ANALYSIS;

ADENOSINE TRIPHOSPHATE; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; CYANOBACTERIA; DNA LIGASES; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PEPTIDE SYNTHASES; PLANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0033850973     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01622.x     Document Type: Article

References (43)

1. Cyanophycin granules from the blue-green alga Anabaena cylindrica: A reserve material consisting of copolymers of aspartic acid and arginine
2. Determination of the structure of the novel polypeptide containing aspartic acid and arginine which is found in cyanobacteria
3. Structural characterization of the cyanophycin granule polypeptide of Anabaena cylindrica by circular dichroism and Raman spectroscopy
4. Structure and composition of cyanophycin granules in the cyanobacterium Aphanocapsa 6308
5. Cyanobacterial cell inclusions
6. Inclusion bodies in the cyanobacteria: Cyanophycin, polyphosphate, polyhedral bodies
7. Inclusions: Cyanophycin
8. 15N isotopic enrichment of cyanophycin synthesized by the cyanobacterium Synechocystis sp. strain PCC 6308
9. Variations in the amino acid composition of cyanophycin in the cyanobacterium Synechocystis sp. PCC 6308 as a function of growth conditions
10. The normal and induced occurence of cyanophycin inclusion bodies in several blue-green algae
11. Absence of the nitrogen reserve polymer cyanophycin from marine Synechococcus species
12. Nitrogen reserves and dynamic reservoirs in cyanobacteria
13. A transposition-induced mutant of Nostoc implicates an arginine-biosynthetic gene in the formation of cyanophycin granules and of functional heterocysts and akinetes
14. The effect of chloramphenicol on the production of cyanophycin granule polypeptide in the blue-green alga Anabaena cylindrica
15. The biosynthesis of multi-L-arginyl-poly (L-aspartic acid) in the filamentous cyanobacterium Anabaena cylindrica
16. Molecular characterization of cyanophycin synthetase, the enzyme catalyzing the biosynthesis of the cyano-bacterial reserve material multi-L-arginyl-poly-L-aspartate (cyanophycin)
17. Glutathione synthesis
18. The chemistry and biosynthesis of selected bacterial capsular polymers
19. Biochemical evidence for the formation of a covalent acyl-phosphate linkage between UDP-N-acetylmuramate and ATP in the Escherichia coli UDP-N-acetylmuramate: L-alanine ligase-catalyzed reaction
20. Formation of adenosine 5'-tetraphosphate from the acyl phosphate intermediate: A difference between MurC and MurD synthetases of Escherichia coli
21. Characteristics of Anabaena variabilis influencing plaque formation by cyanophage N-1
22. Cyanophycinase, a peptidase degrading the cyanobacterial reserve material multi-L-arginyl-poly-L-aspartic acid (cyanophycin). Molecular cloning of the gene of Synechocystis sp. PCC 6803, expression in Escherichia coli, and biochemical characterization of the purified enzyme
23. Photosynthetic activities of a thermophilic blue-green alga
24. Identification of blue-green algal nitrogen fixation genes by using heterologous DNA hybridization probes
25. Biochemistry of microbial polyamide metabolism
26. DNA sequencing with chain-terminating inhibitors
27. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
28. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
29. Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-γ-glutamate ligases: Identification of a ligase superfamily
30. Invariant amino acids in the Mur peptide synthetases of bacterial peptidoglycan synthesis and their modification by site-directed mutagenesis in the UDP-MurNAc:L-alanine ligase from Escherichia coli
31. Crystal structure of UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase from Escherichia coli
32. Improved tools for biological sequence comparison
33. A common fold for peptide synthetases cleaving ATP to ADP: Glutathione synthetase and D-alanine: D-alanine ligase of Escherichia coli
34. Structure of the multifunctional loops in the nonclassical ATP-binding fold of glutathione synthetase
35. Biotin carboxylase comes into the fold
36. Structural classification of proteins: New superfamilies
37. A diverse superfamily of enzymes with ATP-dependent carboxylate-amine/thiol ligase activity
38. Vancomycin resistance: Structure of D-alanine:D-alanine ligase at 2.3 A resolution
39. 5-carboxyaminoimidazole ribonucleotide synthetase: A member of the ATP grasp protein superfamily
40. Mass spectrometry of peptides and proteins by matrix-assisted ultraviolet laser desorption/ionization
41. Purification and characterization of cyanophycin and cyanophycin synthetase from the thermophilic Synechococcus sp. MA19
42. Arginine catabolism in the cyanobacterium Synechocystis sp. strain PCC 6803 involves the urea cycle and arginase pathway