Saturated fatty acids induce insulin resistance in human podocytes: Implications for diabetic nephropathy

Nephrology Dialysis Transplantation

Volumn 24, Issue 11, 2009, Pages 3288-3296

  Lennon, Rachel ^a   Pons, Deborah ^a   Sabin, Matthew A ^a   Wei, Christina ^a   Shield, Julian P ^a   Coward, Richard J ^a   Tavaré, Jeremy M ^a   Mathieson, Peter W ^a   Saleem, Moin A ^a   Welsh, Gavin I ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

Diabetic nephropathy; Glucose uptake; Insulin resistance; Palmitate; Podocyte

Indexed keywords

CERAMIDE; FUMONISIN B1; GLUCOSE TRANSPORTER 4; INSULIN; INSULIN RECEPTOR SUBSTRATE 1; PALMITIC ACID; THERMOZYMOCIDIN; ADENOSINE TRIPHOSPHATE; FATTY ACID; GLUCOSE; SLC2A4 PROTEIN, HUMAN;

ARTICLE; CONTROLLED STUDY; DIABETIC NEPHROPATHY; GENE EXPRESSION; GENETIC TRANSCRIPTION; GLUCOSE TRANSPORT; HUMAN; HUMAN CELL; INSULIN RESISTANCE; INSULIN SENSITIVITY; LIPOGENESIS; PODOCYTE; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN TRANSPORT; BIOSYNTHESIS; CELL LINE; CELL SURVIVAL; DRUG EFFECT; GENE EXPRESSION PROFILING; METABOLISM; PHYSIOLOGY; SIGNAL TRANSDUCTION;

ADENOSINE TRIPHOSPHATE; CELL LINE; CELL SURVIVAL; CERAMIDES; DIABETIC NEPHROPATHIES; FATTY ACIDS; GENE EXPRESSION PROFILING; GLUCOSE; GLUCOSE TRANSPORTER TYPE 4; HUMANS; INSULIN RESISTANCE; PALMITIC ACID; PODOCYTES; SIGNAL TRANSDUCTION;

EID: 73949137331     PISSN: 09310509     EISSN: 14602385     Source Type: Journal    
DOI: 10.1093/ndt/gfp302     Document Type: Article

References (42)

1. Wild S, Roglic G, Green A, et al. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care 2004; 27: 1047-1053
2. Mohn A, Marcovecchio M, Chiarelli F. Validity of HOMA-IR as index of insulin resistance in obesity. J Pediatr 2006; 148: 565-566
3. Kambham N, Markowitz GS, Valeri AM, et al. Obesity-related glomerulopathy: an emerging epidemic. Kidney Int 2001; 59: 1498-1509
4. Becker B, Kronenberg F, Kielstein JT, et al. Renal insulin resistance syndrome, adiponectin and cardiovascular events in patients with kidney disease: the mild and moderate kidney disease study. J Am Soc Nephrol 2005; 16: 1091-1098
5. Dogra GK, Herrmann S, Irish AB, et al. Insulin resistance, dyslipi-daemia, inflammation and endothelial functioninnephrotic syndrome. Nephrol Dial Transplant 2002; 17: 2220-2225
6. Groop L, Ekstrand A, Forsblom C, et al. Insulin resistance, hypertension and microalbuminuria in patients with type 2 (non-insulin-dependent) diabetes mellitus. Diabetologia 1993; 36: 642-647
7. Dimopoulos N, Watson M, Sakamoto K, et al. Differential effects of palmitate on insulin action and glucose utilization in rat L6 skel, et al muscle. Biochem J 2006; 399: 473-481
8. Jebailey L, Wanono O, Niu W, et al. Ceramide-and oxidant-induced insulin resistance involve loss of insulin-dependent rac-activation and actin remodeling in muscle cells. Diabetes 2007; 56: 394-403
9. Schmitz-Peiffer C, Craig DL, Biden TJ. Ceramide generation is sufficient to account for the inhibition of the insulin-stimulated PKB pathway in C2C12 skel, et al muscle cells pretreated with palmitate. J Biol Chem 1999; 274: 24202-24210
10. Wolf G, Chen S, Ziyadeh FN. From the periphery of the glomerular capillary wall toward the center of disease: podocyte injury comes of age in diabetic nephropathy. Diabetes 2005; 54: 1626-1634
11. Wolf G, Ziyadeh FN. Cellular and molecular mechanisms of protein-uria in diabetic nephropathy. Nephron Physiol 2007; 106: p26-p31
12. White KE, Bilous ROB. Structural alterations to the podocyte are related to proteinuria in type 2 diabetic patients. Nephrol Dial Transplant 2004; 19: 1437-1440
13. Patari A, Forsblom C, Havana M, et al. Nephrinuria in diabetic nephropathy of type 1 diabetes. Diabetes 2003; 52: 2969-2974
14. Coward RJ, Welsh GI, Yang J, et al. The human glomerular podocyte is a novel target for insulin action. Diabetes 2005; 54: 3095-3102
15. Coward RJ, Welsh GI, Koziell A, et al. Nephrin is critical for the action of insulin on human glomerular podocytes. Diabetes 2007; 56: 1127-1135
16. Saleem MA, O'Hare MJ, Reiser J, et al. A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression. J Am Soc Nephrol 2002; 13: 630-638
17. O'Hare MJ, Bond J, Clarke C, et al. Conditional immortalization of freshly isolated human mammary fibroblasts and endothelial cells. Proc Natl Acad Sci USA 2001; 98: 646-651
18. Sabin MA, Stewart CE, Crowne EC, et al. Fatty acid-induced defects in insulin signalling, in myotubes derived from children, are related to ceramide production from palmitate rather than the accumulation of intramyocellular lipid. J Cell Physiol 2007; 211: 244-252
19. Sabin MA, De Hora M, Holly JM, et al. Fasting nonesterified fatty acid profiles in childhood and their relationship with adiposity, insulin sensitivity, and lipid levels. Pediatrics 2007; 120: e1426-e1433
20. Mook S, Halkes CJM, Bilecan S, et al. In vivo regulationofplasma-free fatty acids in insulin resistance. Metabolism 2004; 53: 1197-1201
21. Chavez JA, Knotts TA, Wang LP, et al. A role for ceramide, but not diacylglycerol, in the antagonism of insulin signal transduction by saturated fatty acids. J Biol Chem 2003; 278: 10297-10303
22. Merrill AH Jr. De novo sphingolipid biosynthesis: a necessary, but dangerous, pathway. J Biol Chem 2002; 277: 25843-25846
23. Powell DJ, Turban S, Gray A, et al. Intracellular ceramide synthesis and protein kinase Czeta activation play an essential role in palmitate-induced insulin resistance in rat L6 skel, et al muscle cells. Biochem J 2004; 382: 619-629
24. Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 1957; 226: 497-509
25. Bielawska A, Perry DK, Hannun YA. Determination of ceramides and diglycerides by the diglyceride kinase assay. Anal Biochem 2001; 298: 141-150
26. Pessin JE, Saltiel AR. Signaling pathways in insulin action: molecular targets of insulin resistance. J Clin Invest 2000; 106: 165-169
27. Jones N, Blasutig IM, Eremina V, et al. Nck adaptor proteins link nephrin to the actin cytoskeleton of kidney podocytes. Nature 2006; 440: 818-823
28. Marvelle AF, Lange LA, Qin L, et al. Association of FTO with obesity-related traits in the Cebu Longitudinal Health and Nutritional Survey (CLHNS) cohort. Diabetes 2008; 57: 1987-1991
29. Busch CP, Hegele RA. Genetic determinants of type 2 diabetes mel-litus. Clin Genet 2001; 60: 243-254
30. Kestila M, Lenkkeri U, Mannikko M, et al. Positionally cloned gene for a novel glomerular protein-nephrin-is mutated in congenital nephrotic syndrome. Mol Cell 1998; 1: 575-582
31. Patari-Sampo A, Ihalmo P, Holthofer H. Molecular basis of the glomerular filtration: nephrin and the emerging protein complex at the podocyte slit diaphragm. Ann Med 2006; 38: 483-492
32. Yang Q, Graham TE, Mody N, et al. Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes. Nature 2005; 436: 356-362
33. Javor ED, Moran SA, Young JR, et al. Proteinuric nephropathy in acquired and congenital generalized lipodystrophy: baseline characteristics and course during recombinant leptin therapy. J Clin Endocrinol Metab 2004; 89: 3199-3207
34. Storz P, Doppler H, Wernig A, et al. Cross-talk mechanisms in the development of insulin resistance of skel, et al muscle cells palmitate rather than tumour necrosis factor inhibits insulin-dependent protein kinase B (PKB)/Akt stimulation and glucose uptake. Eur J Biochem 1999; 266: 17-25
35. Van Epps-Fung M, Williford J, Wells A, et al. Fatty acid-induced insulin resistance in adipocytes. Endocrinology 1997; 138: 4338-4345
36. Hunnicutt JW, Hardy RW, Williford J, et al. Saturated fatty acid-induced insulin resistance in rat adipocytes. Diabetes 1994; 43: 540-545
37. Torra R. Renal manifestations in Fabry disease and therapeutic options. Kidney Int Suppl 2008; 111: s29-s32
38. Jiang G, Zhang BB. Modulation of insulin signalling by insulin sen-sitizers. Biochem Soc Trans 2005; 33: 358-361
39. Li H, Zhu J, Aoudjit L, et al. Rat nephrin modulates cell morphology via the adaptor protein Nck. Biochem Biophys Res Commun 2006; 349: 310-316
40. Verma R, Kovari I, Soofi A, et al. Nephrin ectodomain engagement results in Src kinase activation, nephrin phosphorylation, Nck recruitment, and actin polymerization. J Clin Invest 2006; 116: 1346-1359
41. Bakris GL, Ruilope LM, McMorn SO, et al. Rosiglitazone reduces microalbuminuria and blood pressure independently of glycemia in type 2 diabetes patients with microalbuminuria. J Hypertens 2006; 24: 2047-2055
42. Nakamura T, Ushiyama C, Osada S, et al. Pioglitazone reduces urinary podocyte excretion in type 2 diabetes patients with microalbuminuria. Metabolism 2001; 50: 1193-1196