Crystallographic analysis of the reaction pathway of Zoogloea ramigera biosynthetic thiolase

Journal of Molecular Biology

Volumn 297, Issue 5, 2000, Pages 1171-1182

  Modis, Yorgo ^a   Wierenga, Rik K ^b  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^b UNIVERSITY OF OULU   (Finland)

Author keywords

Acetyl CoA; Biosynthetic thiolase; Claisen condensation; Oxyanion hole; Reaction intermediate

Indexed keywords

ACETYL COENZYME A; ACETYL COENZYME A ACETYLTRANSFERASE;

ARTICLE; CATALYSIS; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME STRUCTURE; HYDROGEN BOND; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; REACTION ANALYSIS; ZOOGLOEA;

ZOOGLOEA RAMIGERA;

EID: 0034646566     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3638     Document Type: Article

References (28)

1. Characterization of N93S, I312T, and A333P missense mutations in two Japanese families with mitochondrial acetoacetyl-CoA thiolase deficiency
2. Thiolase
3. Purification and crystallization of thiolase; study of its reaction mechanism
4. The relation of acyl transfer to the overall reaction of thiolase I from porcine heart
5. Mitochondrial acetoacetyl-CoA thiolase deficiency
6. Improved methods for building protein models in electron density maps and the location of errors in these models
7. Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants
8. Medium chain 3-ketoacyl-coenzyme A thiolase deficiency: A new disorder of mitochondrial fatty acid β-oxidation
9. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
10. β-Oxidation of fatty acids in mitochondria, peroxisomes, and bacteria: A century of continued progress
11. Automated refinement of protein models
12. PROCHECK: A program to check the stereochemical quality of protein structures
13. Poly-(R)-3-hydroxybutyrate (PHB) biosynthesis: Mechanistic studies on the biological Claisen condensation catalyzed by β-ketoacyl thiolase
14. Bio-Claisen condensation catalyzed by thiolase from Zoogloea ramigera. Active site cysteine residues
15. The 1.8 Å crystal structure of the dimeric peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae: Implications for substrate binding and reaction mechanism
16. Raster 3D photorealistic molecular graphics
17. Properties of peroxisomal 3-ketacyl-CoA thiolase from rat liver
18. A biosynthetic thiolase in complex with a reaction intermediate: The crystal structure provides new insights into the catalytic mechanism
19. Refinement of macromolecular structures by the maximum-likelihood method
20. Structure of triosephosphate isomerase from Escherichia coli determined at 2.6 Å resolution
21. Biosynthetic thiolase from Zoogloea ramigera. Evidence for a mechanism involving Cys378 as the active site base
22. Mechanistic studies on β-ketoacyl thiolase from Zoogloea ramigera: Identification of the active-site nucleophile as Cys89, its mutation to Ser89, and kinetic and thermodynamic characterization of wild-type and mutant enzymes
23. Bacterial metabolism of poly-β-hydroxybutyrate
24. Long-chain 3-hydroxyacyl-coenzyme A dehydrogenase deficiency with the G1528C mutation: Clinical presentation of thirteen patients
25. A very short hydrogen bond provides only moderate stabilization of an enzyme-inhibitor complex of citrate synthase
26. WHAT IF: A molecular modeling and drug design program
27. Enzyme catalyzed aldol and Claisen condensations
28. Biosynthetic thiolase from Zoogloea ramigera. Mutagenesis of the putative active-site base Cys-378 to Ser-378 changes the partitioning of the acetyl S-enzyme intermediate