Intervention of α-lipoic acid ameliorates methotrexate-induced oxidative stress and genotoxicity: A study in rat intestine

Chemico-Biological Interactions

Volumn 183, Issue 1, 2010, Pages 85-97

  Dadhania, V P ^a   Tripathi, D N ^b   Vikram, A ^b   Ramarao, P ^b   Jena, G B ^b  

^a Johnson and Johnson Limited   (India)

^b NATIONAL INSTITUTE OF PHARMACEUTICAL EDUCATION AND RESEARCH NIPER   (India)

Author keywords

Lipoic acid; Comet assay; Intestine; Methotrexate; Oxidative stress; Rat

Indexed keywords

CAS 59 05 2; GLUTATHIONE; MALONALDEHYDE; METHOTREXATE; PROTEIN P53; THIOCTIC ACID; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; CELL DAMAGE; CELL PROTECTION; CELL STRUCTURE; CONTROLLED STUDY; CYTOTOXICITY; DIARRHEA; DIGESTIVE SYSTEM INFLAMMATION; DISEASE SEVERITY; DNA DAMAGE; DOSE RESPONSE; DRUG EFFECT; GASTROINTESTINAL TOXICITY; GENOTOXICITY; INTESTINE CELL; MALE; MORTALITY; NONHUMAN; OXIDATIVE STRESS; RAT; SHORT COURSE THERAPY; SURVIVAL RATE; TREATMENT DURATION;

ALKALINE PHOSPHATASE; ANIMALS; ANTIMETABOLITES, ANTINEOPLASTIC; COMET ASSAY; DNA DAMAGE; GLUTATHIONE; IN SITU NICK-END LABELING; INTESTINES; MALE; MALONDIALDEHYDE; METHOTREXATE; OXIDATIVE STRESS; RATS; RATS, SPRAGUE-DAWLEY; THIOCTIC ACID; TUMOR SUPPRESSOR PROTEIN P53;

ANIMALIA; RATTUS;

EID: 71549114179     PISSN: 00092797     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cbi.2009.10.020     Document Type: Article

References (78)

1. Ortiz Z., Shea B., Suarez-Almazor M.E., Moher D., Wells G.A., and Tugwell P. The efficacy of folic acid and folinic acid in reducing methotrexate gastrointestinal toxicity in rheumatoid arthritis. A metaanalysis of randomized controlled trials. J. Rheumatol. 25 1 (1998) 36-43
2. Genestier L., Paillot R., Quemeneur L., Izeradjene K., and Revillard J.P. Mechanisms of action of methotrexate. Immunopharmacology 47 2-3 (2000) 247-257
3. Grosflam J., and Weinblatt M.E. Methotrexate: mechanism of action, pharmacokinetics, clinical indications, and toxicity. Curr. Opin. Rheumatol. 3 3 (1991) 363-368
4. Goodman T.A., and Polisson R.P. Methotrexate: adverse reactions and major toxicities. Rheum. Dis. Clin. North Am. 20 2 (1994) 513-528
5. Altmann G.G. Changes in the mucosa of the small intestine following methotrexate administration or abdominal x-irradiation. Am. J. Anat. 140 2 (1974) 263-279
6. Ciralik H., Bulbuloglu E., Cetinkaya A., Kurutas E.B., Celik M., and Polat A. Effects of N-acetylcysteine on methotrexate-induced small intestinal damage in rats. Mt. Sinai J. Med. 73 8 (2006) 1086-1092
7. Gibson R.J., Keefe D.M., Clarke J.M., Regester G.O., Thompson F.M., Goland G.J., Edwards B.G., and Cummins A.G. The effect of keratinocyte growth factor on tumour growth and small intestinal mucositis after chemotherapy in the rat with breast cancer. Cancer Chemother. Pharmacol. 50 1 (2002) 53-58
8. Kolli V.K., Abraham P., and Rabi S. Methotrexate-induced nitrosative stress may play a critical role in small intestinal damage in the rat. Arch. Toxicol. 82 10 (2008) 763-770
9. Kato S., Ito K., Kato Y., Wakayama T., Kubo Y., Iseki S., and Tsuji A. Involvement of multidrug resistance-associated protein 1 in intestinal toxicity of methotrexate. Pharma. Res. 26 6 (2009) 1467-1476
10. Keshava C., Keshava N., Whong W.Z., Nath J., and Ong T.M. Inhibition of methotrexate-induced chromosomal damage by vanillin and chlorophyllin in V79 cells. Teratog. Carcinog. Mutagen. 17 6 (1997) 313-326
11. Padmanabhan S., Tripathi D.N., Vikram A., Ramarao P., and Jena G.B. Cytotoxic and genotoxic effects of methotrexate in germ cells of male Swiss mice. Mutat. Res. 655 1-2 (2008) 59-67
12. Padmanabhan S., Tripathi D.N., Vikram A., Ramarao P., and Jena G.B. Methotrexate-induced cytotoxicity and genotoxicity in germ cells of mice: intervention of folic and folinic acid. Mutat. Res. 673 1 (2009) 43-52
13. Kolgazi M., Jahovic N., Yuksel M., Ercan F., and Alican I. Alpha-lipoic acid modulates gut inflammation induced by trinitrobenzene sulfonic acid in rats. J. Gastroenterol. Hepatol. 22 11 (2007) 1859-1865
14. Packer L., Witt E.H., and Tritschler H.J. alpha-Lipoic acid as a biological antioxidant. Free Rad. Biol. Med. 19 2 (1995) 227-250
15. Moini H., Packer L., and Saris N.E. Antioxidant and prooxidant activities of alpha-lipoic acid and dihydrolipoic acid. Toxicol. Appl. Pharmacol. 182 1 (2002) 84-90
16. Biewenga G.P., Haenen G.R., and Bast A. The pharmacology of the antioxidant lipoic acid. Gen. Pharmacol. 29 3 (1997) 315-331
17. Marsh S.A., Laursen P.B., Pat B.K., Gobe G.C., and Coombes J.S. Bcl-2 in endothelial cells is increased by vitamin E and alpha-lipoic acid supplementation but not exercise training. J. Mol. Cell. Cardiol. 38 3 (2005) 445-451
18. Yanez J.A., Teng X.W., Roupe K.A., Fariss M.W., and Davies N.M. Chemotherapy induced gastrointestinal toxicity in rats: involvement of mitochondrial DNA, gastrointestinal permeability and cyclooxygenase-2. J. Pharm. Pharm. Sci. 6 3 (2003) 308-314
19. Bajin-Katic K., Stankov K., {Eth}olai M., and Kovacevic Z. Intestinal alkaline phosphatase activity as a molecular marker of enterotoxicity induced by single dose of 5-fluorouracil and protective role of orally administered glutamine. Arch. Oncol. 14 (2006) 101-105
20. Winton D.J., and Brooks R.A. Analysis of DNA damage and repair accompanying differentiation in the intestinal crypt. Philos. Trans. R. Soc. Lond. B: Biol. Sci. 353 1370 (1998) 895-902
21. Ohkawa H., Ohishi N., and Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95 2 (1979) 351-358
22. Moron M.S., Depierre J.W., and Mannervik B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochim. Biophys. Acta 582 1 (1979) 67-78
23. Lowry O.H., Rosebrough N.J., Farr A.L., and Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193 1 (1951) 265-275
24. Singh N.P., McCoy M.T., Tice R.R., and Schneider E.L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175 1 (1988) 184-191
25. Tripathi D.N., Pawar A.A., Vikram A., Ramarao P., and Jena G.B. Use of the alkaline comet assay for the detection of transplacental genotoxins in newborn mice. Mutat. Res. 653 1-2 (2008) 134-139
26. Vikram A., Tripathi D.N., Ramarao P., and Jena G.B. Evaluation of streptozotocin genotoxicity in rats from different ages using the micronucleus assay. Regul. Toxicol. Pharmacol. 49 3 (2007) 238-244
27. Vikram A., Tripathi D.N., Ramarao P., and Jena G.B. Intervention of D-glucose ameliorates the toxicity of streptozotocin in accessory sex organs of rat. Toxicol. Appl. Pharmacol. 226 1 (2008) 84-93
28. Graham M.F., Diegelmann R.F., Elson C.O., Lindblad W.J., Gotschalk N., Gay S., and Gay R. Collagen content and types in the intestinal strictures of Crohn's disease. Gastroenterology 94 2 (1988) 257-265
29. Sener G., Eksioglu-Demiralp E., Cetiner M., Ercan F., and Yegen B.C. Beta-glucan ameliorates methotrexate-induced oxidative organ injury via its antioxidant and immunomodulatory effects. Eur. J. Pharmacol. 542 1-3 (2006) 170-178
30. Vardi N., Parlakpinar H., Ozturk F., Ates B., Gul M., Cetin A., Erdogan A., and Otlu A. Potent protective effect of apricot and beta-carotene on methotrexate-induced intestinal oxidative damage in rats. Food Chem. Toxicol. 46 9 (2008) 3015-3022
31. Barrios L., and Poletti O.H. Effects of filgrastim on granulopoietic cells of mice pretreated with methotrexate. Biocell 29 1 (2005) 7-14
32. Chow M., Koo J., Ng P., and Rubin H. Random population-wide genetic damage induced in replicating cells treated with methotrexate. Mutat. Res. 413 3 (1998) 251-264
33. Miyazaki J., Kawai K., Hayashi H., Onozawa M., Tsukamoto S., Miyanaga N., Hinotsu S., Shimazui T., and Akaza H. The limited efficacy of methotrexate, actinomycin D and cisplatin (MAP) for patients with advanced testicular cancer. Jpn. J. Clin. Oncol. 33 8 (2003) 391-395
34. Uwai Y., Saito H., and Inui K. Interaction between methotrexate and nonsteroidal anti-inflammatory drugs in organic anion transporter. Eur. J. Pharmacol. 409 1 (2000) 31-36
35. Kamen B.A., Nylen P.A., Camitta B.M., and Bertino J.R. Methotrexate accumulation and folate depletion in cells as a possible mechanism of chronic toxicity to the drug. Br. J. Haematol. 49 3 (1981) 355-360
36. Novakovic T., Miloševic-{Eth}ordevic O., Grujicic D., Marinkovic D., Jankovic S., and Arsenijevic S. Effect of intratumoral application of methotrexate in vivo on frequency of micronuclei in peripheral blood lymphocytes. Arch. Oncol. 11 (2003) 1-4
37. Jahovic N., Sener G., Cevik H., Ersoy Y., Arbak S., and Yegen B.C. Amelioration of methotrexate-induced enteritis by melatonin in rats. Cell Biochem. Funct. 22 3 (2004) 169-178
38. Yuncu M., Eralp A., and Celik A. Effect of aged garlic extract against methotrexate-induced damage to the small intestine in rats. Phytother. Res. 20 6 (2006) 504-510
39. Sogabe N., Mizoi L., Asahi K., Ezawa I., and Goseki-Sone M. Enhancement by lactose of intestinal alkaline phosphatase expression in rats. Bone 35 1 (2004) 249-255
40. Islam M.S., Murata T., Fujisawa M., Nagasaka R., Ushio H., Bari A.M., Hori M., and Ozaki H. Anti-inflammatory effects of phytosteryl ferulates in colitis induced by dextran sulphate sodium in mice. Br. J. Pharmacol. 154 4 (2008) 812-824
41. Kanauchi O., Serizawa I., Araki Y., Suzuki A., Andoh A., Fujiyama Y., Mitsuyama K., Takaki K., Toyonaga A., Sata M., and Bamba T. Germinated barley foodstuff, a prebiotic product, ameliorates inflammation of colitis through modulation of the enteric environment. J. Gastroenterol. 38 2 (2003) 134-141
42. Souza D.G., Guabiraba R., Pinho V., Bristow A., Poole S., and Teixeira M.M. IL-1-driven endogenous IL-10 production protects against the systemic and local acute inflammatory response following intestinal reperfusion injury. J. Immunol. 170 9 (2003) 4759-4766
43. Reifen R., Nur T., Ghebermeskel K., Zaiger G., Urizky R., and Pines M. Vitamin A deficiency exacerbates inflammation in a rat model of colitis through activation of nuclear factor-kappaB and collagen formation. J. Nutr. 132 9 (2002) 2743-2747
44. Uni Z., Zaiger G., and Reifen R. Vitamin A deficiency induces morphometric changes and decreased functionality in chicken small intestine. Br. J. Nutr. 80 4 (1998) 401-407
45. Jung Y.H., Nam K.I., Oh C.S., Ahn K.Y., Bae C.S., Kim B.Y., and Park S.S. Light and electron microscopic study on the mode of cell death induced by methotrexate administration in the crypt and villus of rat small intestine. Korean J. Anat. 32 3 (1999) 305-321
46. Gibson R.J., Bowen J.M., Cummins A.G., and Keefe D.M. Relationship between dose of methotrexate, apoptosis, p53/p21 expression and intestinal crypt proliferation in the rat. Clin. Exp. Med. 4 4 (2005) 188-195
47. Okudela K., Ito T., Mitsui H., Hayashi H., Udaka N., Kanisawa M., and Kitamura H. The role of p53 in bleomycin-induced DNA damage in the lung. A comparative study with the small intestine. Am. J. Pathol. 155 4 (1999) 1341-1351
48. Slupphaug G., Kavli B., and Krokan H.E. The interacting pathways for prevention and repair of oxidative DNA damage. Mutat. Res. 531 1-2 (2003) 231-251
49. Valko M., Izakovic M., Mazur M., Rhodes C.J., and Telser J. Role of oxygen radicals in DNA damage and cancer incidence. Mol. Cell. Biochem. 266 1-2 (2004) 37-56
50. Lee J.D., Huh J.E., Baek Y.H., Cho K.C., Choi D.Y., and Park D.S. The efficacy and mechanism action of RvCSd, a new herbal agent, on immune suppression and cartilage protection in a mouse model of rheumatoid arthritis. J. Pharmacol. Sci. 109 2 (2009) 211-221
51. Nariya M., Shukla V., Jain S., and Ravishankar B. Comparison of enteroprotective efficacy of triphala formulations (Indian Herbal Drug) on methotrexate-induced small intestinal damage in rats. Phytother. Res. 23 8 (2009) 1092-1098
52. Horie T., Li T., Ito K., Sumi S., and Fuwa T. Aged garlic extract protects against methotrexate-induced apoptotic cell injury of IEC-6 cells. J. Nutr. 136 (2006) 861S-863S
53. Li T., Ito K., Sumi S., Fuwa T., and Horie T. Protective effect of aged garlic extract (AGE) on the apoptosis of intestinal epithelial cells caused by methotrexate. Cancer Chemother. Pharmacol. 63 5 (2009) 873-880
54. Southcott E., Tooley K.L., Howarth G.S., Davidson G.P., and Butler R.N. Yoghurts containing probiotics reduce disruption of the small intestinal barrier in methotrexate-treated rats. Dig. Dis. Sci. 53 7 (2008) 1837-1841
55. Uraz S., Tahan V., Aygun C., Eren F., Unluguzel G., Yuksel M., Senturk O., Avsar E., Haklar G., Celikel C., Hulagu S., and Tozun N. Role of ursodeoxycholic acid in prevention of methotrexate-induced liver toxicity. Dig. Dis. Sci. 53 4 (2008) 1071-1077
56. Cetin A., Kaynar L., Kocyigit I., Hacioglu S.K., Saraymen R., Ozturk A., Sari I., and Sagdic O. Role of grape seed extract on methotrexate-induced oxidative stress in rat liver. Am J. Chin. Med. 36 5 (2008) 861-872
57. Sukhotnik I., Shehadeh N., Coran A.G., Mogilner J.G., Karry R., Shamian B., Ure B.M., and Shamir R. Oral insulin enhances cell proliferation and decreases enterocyte apoptosis during methotrexate-induced mucositis in the rat. J. Ped. Gastroenterol. Nutr. 47 2 (2008) 115-122
58. Madhyastha S., Prabhu L.V., Saralaya V., and Rai R. A comparison of vitamin A and leucovorin for the prevention of methotrexate-induced micronuclei production in rat bone marrow. Clinics 63 6 (2008) 821-826
59. Warden R.A., Noltorp R.S., Francis J.L., Dunkley P.R., and O'Loughlin E.V. Vitamin A deficiency exacerbates methotrexate-induced jejunal injury in rats. J. Nutr. 127 5 (1997) 770-776
60. Sener G., Eksioglu-Demiralp E., Cetiner M., Ercan F., Sirvanci S., Gedik N., and Yegen B.C. L-Carnitine ameliorates methotrexate-induced oxidative organ injury and inhibits leukocyte death. Cell Biol. Toxicol. 22 1 (2006) 47-60
61. Tooley K.L., Howarth G.S., Lymn K.A., Lawrence A., and Butler R.N. Oral ingestion of streptococcus thermophilus diminishes severity of small intestinal mucositis in methotrexate treated rats. Cancer Biol. Ther. 5 6 (2006) 593-600
62. Cetiner M., Sener G., Sehirli A.O., Eksioglu-Demiralp E., Ercan F., Sirvanci S., Gedik N., Akpulat S., Tecimer T., and Yegen B.C. Taurine protects against methotrexate-induced toxicity and inhibits leukocyte death. Toxicol. Appl. Pharmacol. 209 1 (2005) 39-50
63. van't Land B., van Beek N.M., van den Berg J.J., and M'Rabet L. Lactoferrin reduces methotrexate-induced small intestinal damage, possibly through inhibition of GLP-2-mediated epithelial cell proliferation. Dig. Dis. Sci. 49 3 (2004) 425-433
64. Mitsugi K., Nakamura T., Kashiwabara N., Ariyama H., Tanaka R., Baba E., Nakamura M., Harada M., and Nakano S. Protection against methotrexate toxicity by a soybean protein- and omega-3 fatty acid-containing diet: comparative study with a casein-containing diet. Oncol. Rep. 12 1 (2004) 41-45
65. Jahovic N., Cevik H., Sehirli A.O., Yegen B.C., and Sener G. Melatonin prevents methotrexate-induced hepatorenal oxidative injury in rats. J. Pin. Res. 34 4 (2003) 282-287
66. Drachtman R.A., Cole P.D., Golden C.B., James S.J., Melnyk S., Aisner J., and Kamen B.A. Dextromethorphan is effective in the treatment of subacute methotrexate neurotoxicity. Ped. Hematol. Oncol. 19 5 (2002) 319-327
67. Muralikrishnan G., Amalan Stanley V., and Sadasivan Pillai K. Dual role of vitamin C on lipid profile and combined application of cyclophosphamide, methotrexate and 5-fluorouracil treatment in fibrosarcoma-bearing rats. Cancer lett. 169 2 (2001) 115-120
68. Shahin A.A., Ismail M.M., Saleh A.M., Moustafa H.A., Aboul-Ella A.A., and Gabr H.M. Protective effect of folinic acid on low-dose methotrexate genotoxicity. Z. Rheumatol. 60 2 (2001) 63-68
69. Marks S.L., Cook A.K., Reader R., Kass P.H., Theon A.P., Greve C., and Rogers Q.R. Effects of glutamine supplementation of an amino acid-based purified diet on intestinal mucosal integrity in cats with methotrexate-induced enteritis. Am. J. Vet. Res. 60 6 (1999) 755-763
70. Logvinova A.V., Foehr M.W., Pemberton P.A., Khazalpour K.M., Funk-Archuleta M.A., Bathurst I.C., and Tomei L.D. Soy-derived antiapoptotic fractions protect gastrointestinal epithelium from damage caused by methotrexate treatment in the rat. Nutr. Cancer 33 1 (1999) 33-39
71. Kanauchi O., Mitsuyama K., Saiki T., Agata K., Nakamura T., and Iwanaga T. Preventive effects of germinated barley foodstuff on methotrexate-induced enteritis in rats. Int. J. Mol. Med. 1 6 (1998) 961-966
72. Horie T., Nakamaru M., and Masubuchi Y. Docosahexaenoic acid exhibits a potent protection of small intestine from methotrexate-induced damage in mice. Life Sci. 62 15 (1998) 1333-1338
73. Howarth G.S., Francis G.L., Cool J.C., Xu X., Byard R.W., and Read L.C. Milk growth factors enriched from cheese whey ameliorate intestinal damage by methotrexate when administered orally to rats. J. Nutr. 126 10 (1996) 2519-2530
74. Chevreau N., and Funk-Archuleta M. Effect of enteral formulas on methotrexate toxicity. Nutr. Cancer 23 2 (1995) 185-204
75. Subramaniam S., Subramaniam S., and Shyamala Devi C.S. Vitamin E protects intestinal basolateral membrane from CMF-induced damages in rat. Ind. J. Physiol. Pharmacol. 39 3 (1995) 263-266
76. Oredipe O.A., White S.L., Grzegorzewski K., Gause B.L., Cha J.K., Miles V.A., and Olden K. Protective effects of swainsonine on murine survival and bone marrow proliferation during cytotoxic chemotherapy. J. Nat. Cancer Inst. 83 16 (1991) 1149-1156
77. Vanderhoof J.A., Park J.H., Mohammadpour H., and Blackwood D. Effects of dietary lipids on recovery from mucosal injury. Gastroenterology 98 5 Pt 1 (1990) 1226-1231
78. Badr M.Z., and Chen T.S. Prevention of methotrexate-induced gastrointestinal toxicity by glucose. Drug Nutr. Interact. 5 1 (1987) 43-47