Cell-free production of Gaussia princeps luciferase - antibody fragment bioconjugates for ex vivo detection of tumor cells

Biochemical and Biophysical Research Communications

Volumn 390, Issue 3, 2009, Pages 971-976

  Patel, Kedar G ^a   Ng, Patrick P ^b   Kuo, Chiung Chi ^b   Levy, Shoshana ^b   Levy, Ronald ^b   Swartz, James R ^a,c  

^a Stanford University   (United States)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c NONE   (United States)

Author keywords

Bioconjugate; Bioluminescence; Cell free protein synthesis (CFPS); Cell surface binding assay; Gaussia princeps luciferase; Non natural amino acids

Indexed keywords

ALKYNE; AMINO ACID; AMINO ACID TRANSFER RNA LIGASE; AZIDE; GAUSSIA PRINCEPS LUCIFERASE; HYBRID PROTEIN; LUCIFERASE; METHIONINE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; TRANSFER RNA; TYROSINE DERIVATIVE; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARCHAEBACTERIUM; ARTICLE; BIOLUMINESCENCE; CELL FREE SYSTEM; CELL SURFACE; CONJUGATION; CONTROLLED STUDY; COPEPOD; COVALENT BOND; DISULFIDE BOND; DNA SEQUENCE; ESCHERICHIA COLI; EX VIVO STUDY; EXPRESSION VECTOR; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; REPORTER GENE; TUMOR CELL; TUMOR DIAGNOSIS;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, NEOPLASM; COPEPODA; ESCHERICHIA COLI; HUMANS; IMMUNOGLOBULIN IDIOTYPES; IMMUNOGLOBULIN VARIABLE REGION; LUCIFERASES; LYMPHOMA, B-CELL; MICE; MOLECULAR SEQUENCE DATA; PROTEIN BIOSYNTHESIS; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS;

ARCHAEA; ESCHERICHIA COLI; GAUSSIA PRINCEPS (T. SCOTT, 1894);

EID: 70449701405     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2009.10.087     Document Type: Article

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