Fusion of Gaussia Luciferase to an engineered anti-carcinoembryonic antigen (CEA) antibody for in vivo optical imaging

Molecular Imaging and Biology

Volumn 9, Issue 5, 2007, Pages 267-277

  Venisnik, Katy M ^a   Olafsen, Tove ^a   Gambhir, Sanjiv S ^b   Wu, Anna M ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Bioluminescence; Carcinoembryonic antigen; Engineered antibodies; Gaussia luciferase; Nude mouse xenograft models

Indexed keywords

CARCINOEMBRYONIC ANTIBODY LUCIFERASE FUSION PROTEIN I 124; CARCINOEMBRYONIC ANTIGEN; RADIOPHARMACEUTICAL AGENT; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BIOLUMINESCENCE; COLON CARCINOMA; CONTROLLED STUDY; DIAGNOSTIC VALUE; DRUG UPTAKE; ENZYME STABILITY; FEMALE; GLIOMA; HUMAN; HUMAN CELL; IN VIVO STUDY; MOLECULAR IMAGING; MOUSE; NONHUMAN; POSITRON EMISSION TOMOGRAPHY; PRIORITY JOURNAL; PROCESS OPTIMIZATION; RAT; STATISTICAL SIGNIFICANCE; TUMOR XENOGRAFT;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, NEOPLASM; BASE SEQUENCE; CARCINOEMBRYONIC ANTIGEN; CELL LINE, TUMOR; COPEPODA; DNA PRIMERS; FEMALE; HUMANS; IMMUNOGLOBULIN FRAGMENTS; IODINE RADIOISOTOPES; LUCIFERASES; LUMINESCENT MEASUREMENTS; MICE; MICE, NUDE; MOLECULAR SEQUENCE DATA; NEOPLASM TRANSPLANTATION; NEOPLASMS, EXPERIMENTAL; POSITRON-EMISSION TOMOGRAPHY; PROTEIN ENGINEERING; RADIOPHARMACEUTICALS; RATS; RECOMBINANT FUSION PROTEINS; TRANSPLANTATION, HETEROLOGOUS;

MUS; MUS MUSCULUS;

EID: 34548155475     PISSN: 15361632     EISSN: 18602002     Source Type: Journal    
DOI: 10.1007/s11307-007-0101-8     Document Type: Article

References (23)

1. Zhao H, Doyle TC, Coquoz O, et al. (2005) Emission spectra of bioluminescent reporters and interaction with mammalian tissue determine the sensitivity of detection in vivo. J Biomed Opt 10(4):41210
2. Loening AM, Fenn TD, Wu AM, Gambhir SS (2006) Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output. Protein Eng Des Sel 19(9):391-400
3. Holliger P, Prospero T, Winter G (1993) Diabodies: Small bivalent and bispecific antibody fragments. Proc Natl Acad Sci USA 90(14):6444-6448
4. Wu AM, Williams LE, Zieran L, et al. (1999) Anti-carcinoembryonic antigen (CEA) diabody for rapid tumor targeting and imaging. Tumor Targeting 4:47-58
5. Sundaresan G, Yazaki PJ, Shively JE, et al. (2003) 124I-labeled engineered anti-CEA minibodies and diabodies allow high-contrast, antigen-specific small-animal PET imaging of xenografts in athymic mice. J Nucl Med 44(12):1962-1969
6. Lorenz WW, Cormier MJ, O'Kane DJ, et al. (1996) Expression of the Renilla reniformis luciferase gene in mammalian cells. J Biolumin Chemilumin 11(1):31-37
7. Massoud TF, Gambhir SS (2003) Molecular imaging in living subjects: seeing fundamental biological processes in a new light. Genes Dev 17(5):545-580
8. Shah K, Bureau E, Kim DE, et al. (2005) Glioma therapy and real-time imaging of neural precursor cell migration and tumor regression. Ann Neurol 57(1):34-41
9. Gross S, Piwnica-Worms D (2005) Spying on cancer: Molecular imaging in vivo with genetically encoded reporters. Cancer Cell 7(1):5-15
10. Bhaumik S, Gambhir SS (2002) Optical imaging of Renilla luciferase reporter gene expression in living mice. Proc Natl Acad Sci USA 99(1):377-382
11. Bhaumik S, Lewis XZ, Gambhir SS (2004) Optical imaging of Renilla luciferase, synthetic Renilla luciferase, and firefly luciferase reporter gene expression in living mice. J Biomed Opt 9(3):578-586
12. Matthews JC, Hori K, Cormier MJ (1977) Substrate and substrate analogue binding properties of Renilla luciferase. Biochemistry 16(24):5217-5220
13. Verhaegent M, Christopoulos TK (2002) Recombinant Gaussia luciferase. Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization. Anal Chem 74(17):4378-4385
14. Tannous BA, Kim DE, Fernandez JL, Weissleder R, Breakefield XO (2005) Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo. Mol Ther 11(3):435-443
15. Venisnik KM, Olafsen T, Loening AM, et al. (2006) Bifunctional antibody-Renilla luciferase fusion protein for in vivo optical detection of tumors. Protein Eng Des Sel 19(10):453-460
16. Takkinen K, Laukkanen ML, Sizmann D, et al. (1991) An active single-chain antibody containing a cellulase linker domain is secreted by Escherichia coli. Protein Eng 4(7):837-841
17. Yazaki PJ, Shively L, Clark C, et al. (2001) Mammalian expression and hollow fiber bioreactor production of recombinant anti-CEA diabody and minibody for clinical applications. J Immunol Methods 253(1-2):195-208
18. Wu AM, Yazaki PJ, Tsai S, et al. (2000) High-resolution microPET imaging of carcinoembryonic antigen-positive xenografts by using a copper-64-labeled engineered antibody fragment. Proc Natl Acad Sci USA 97(15):8495-8500
19. Kenanova V, Olafsen T, Crow DM, et al. (2005) Tailoring the pharmacokinetics and positron emission tomography imaging properties of anti-carcinoembryonic antigen single-chain Fv-Fc antibody fragments. Cancer Res 65(2):622-631
20. Chow PL, Rannou FR, Chatziioannou AF (2005) Attenuation correction for small animal PET tomographs. Phys Med Biol 50(8):1837-1850
21. Defrise M, Kinahan PE, Townsend DW, et al. (1997) Exact and approximate rebinning algorithms for 3-D PET data. IEEE Trans Med Imaging 16(2):145-158
22. Kinahan PE, Rogers JG (1989) Analytic 3D image reconstruction using all detected events. IEEE Trans Nucl Sci 36(1):964-968
23. Loening AM, Gambhir SS (2003) AMIDE: A free software tool for multimodality medical image analysis. Molecular Imaging 2(3):131-137