Multiply mutated Gaussia luciferases provide prolonged and intense bioluminescence

Biochemical and Biophysical Research Communications

Volumn 389, Issue 4, 2009, Pages 563-568

  Welsh, John P ^a   Patel, Kedar G ^a   Manthiram, Karthish ^a   Swartz, James R ^a  

^a Stanford University   (United States)

Author keywords

Bioconjugation; Bioluminescence kinetics; Cell free protein synthesis; Gaussia luciferase; Non natural amino acids

Indexed keywords

AMINO ACID; AZIDE; GLYCINE; HOMOPROPARGLYGLYCINE; LUCIFERASE; UNCLASSIFIED DRUG;

ARTICLE; BIOLUMINESCENCE; CHEMICAL STRUCTURE; CONTROLLED STUDY; COPEPOD; GAUSSIA; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; PRIORITY JOURNAL; SYNTHESIS;

AMINO ACID SEQUENCE; ANIMALS; COPEPODA; LUCIFERASES; LUMINESCENCE; MOLECULAR SEQUENCE DATA; MUTANT PROTEINS; MUTATION;

COPEPODA; GAUSSIA (COPEPOD); GAUSSIA PRINCEPS (T. SCOTT, 1894);

EID: 70349751620     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2009.09.006     Document Type: Article

References (29)

1. Contag C.H., and Ross B.D. It's not just about anatomy: in vivo bioluminescence imaging as an eyepiece into biology. J. Magn. Reson. Imaging 16 (2002) 378-387
2. Luker K.E., and Luker G.D. Applications of bioluminescence imaging to antiviral research and therapy: multiple luciferase enzymes and quantitation. Antiviral Res. 78 (2008) 179-187
3. Tannous B.A., Kim D.E., Fernandez J.L., Weissleder R., and Breakefield X.O. Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo. Mol. Ther. 11 (2005) 435-443
4. Villalobos V., Naik S., and Piwnica-Worms D. Current state of imaging protein-protein interactions in vivo with genetically encoded reporters. Annu. Rev. Biomed. Eng. 9 (2007) 321-349
5. Wurdinger T., Badr C., Pike L., de Kleine R., Weissleder R., Breakefield X.O., and Tannous B.A. A secreted luciferase for ex vivo monitoring of in vivo processes. Nat. Methods 5 (2008) 171-173
6. Lee C., Kim J., Do H., and Hwang S. Monitoring thiocyanate-degrading microbial community in relation to changes in process performance in mixed culture systems near washout. Water Res. 42 (2008) 1254-1262
7. Bhaumik S., and Gambhir S.S. Optical imaging of Renilla luciferase reporter gene expression in living mice. Proc. Natl. Acad. Sci. USA 99 (2002) 377-382
8. de Wet J.R., Wood K.V., Helinski D.R., and DeLuca M. Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli. Proc. Natl. Acad. Sci. USA 82 (1985) 7870-7873
9. Fraga H. Firefly luminescence: a historical perspective and recent developments. Photochem. Photobiol. Sci. 7 (2008) 146-158
10. Lorenz W.W., McCann R.O., Longiaru M., and Cormier M.J. Isolation and expression of a cDNA encoding Renilla reniformis luciferase. Proc. Natl. Acad. Sci. USA 88 (1991) 4438-4442
11. Goerke A.R., Loening A.M., Gambhir S.S., and Swartz J.R. Cell-free metabolic engineering promotes high-level production of bioactive Gaussia princeps luciferase. Metab. Eng. 10 (2008) 187-200
12. Venisnik K.M., Olafsen T., Gambhir S.S., and Wu A.M. Fusion of Gaussia luciferase to an engineered anti-carcinoembryonic antigen (CEA) antibody for in vivo optical imaging. Mol. Imaging Biol. 9 (2007) 267-277
13. Maguire C.A., Deliolanis N.C., Pike L., Niers J.M., Tjon-Kon-Fat L.A., Sena-Esteves M., and Tannous B.A. Gaussia luciferase variant for high-throughput functional screening applications. Anal. Chem. 81 (2009) 7102-7106
14. Wuu J.J., and Swartz J.R. High yield cell-free production of integral membrane proteins without refolding or detergents. Biochim. Biophys. Acta 1778 (2008) 1237-1250
15. Bundy B.C., Franciszkowicz M.J., and Swartz J.R. Escherichia coli-based cell-free synthesis of virus-like particles. Biotechnol. Bioeng. 100 (2008) 28-37
16. Yang J., Kanter G., Voloshin A., Michel-Reydellet N., Velkeen H., Levy R., and Swartz J.R. Rapid expression of vaccine proteins for B-cell lymphoma in a cell-free system. Biotechnol. Bioeng. 89 (2005) 503-511
17. Deiters A., and Schultz P.G. In vivo incorporation of an alkyne into proteins in Escherichia coli. Bioorg. Med. Chem. Lett. 15 (2005) 1521-1524
18. Strable E., Prasuhn Jr. D.E., Udit A.K., Brown S., Link A.J., Ngo J.T., Lander G., Quispe J., Potter C.S., Carragher B., Tirrell D.A., and Finn M.G. Unnatural amino acid incorporation into virus-like particles. Bioconjug. Chem. 19 (2008) 866-875
19. Chin J.W., Santoro S.W., Martin A.B., King D.S., Wang L., and Schultz P.G. Addition of p-azido-l-phenylalanine to the genetic code of Escherichia coli. J. Am. Chem. Soc. 124 (2002) 9026-9027
20. Jewett M.C., and Swartz J.R. Mimicking the Escherichia coli cytoplasmic environment activates long-lived and efficient cell-free protein synthesis. Biotechnol. Bioeng. 86 (2004) 19-26
21. Kim D.M., and Swartz J.R. Efficient production of a bioactive, multiple disulfide-bonded protein using modified extracts of Escherichia coli. Biotechnol. Bioeng. 85 (2004) 122-129
22. Yin G., and Swartz J.R. Enhancing multiple disulfide bonded protein folding in a cell-free system. Biotechnol. Bioeng. 86 (2004) 188-195
23. Goerke A.R., and Swartz J.R. Development of cell-free protein synthesis platforms for disulfide bonded proteins. Biotechnol. Bioeng. 99 (2008) 351-367
24. Calhoun K.A., and Swartz J.R. An economical method for cell-free protein synthesis using glucose and nucleoside monophosphates. Biotechnol. Prog. 21 (2005) 1146-1153
25. O'Kane D.J., and Lee J. Absolute calibration of luminometers with low-level light standards. Methods Enzymol. 305 (2000) 87-96
26. Zhou Z., and Fahrni C.J. A fluorogenic probe for the copper(I)-catalyzed azide-alkyne ligation reaction: modulation of the fluorescence emission via 3(n, pi)-1(pi, pi) inversion. J. Am. Chem. Soc. 126 (2004) 8862-8863
27. Inouye S., and Sahara Y. Identification of two catalytic domains in a luciferase secreted by the copepod Gaussia princeps. Biochem. Biophys. Res. Commun. 365 (2008) 96-101
28. Kyte J., and Doolittle R.F. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157 (1982) 105-132
29. Matthews J.C., Hori K., and Cormier M.J. Substrate and substrate analogue binding properties of Renilla luciferase. Biochemistry 16 (1977) 5217-5220