Capillary and microchip electrophoresis for rapid detection of known mutations by combining allele-specific DNA amplification with heteroduplex analysis

Clinical Chemistry

Volumn 47, Issue 2, 2001, Pages 173-185

  Tian, H ^a   Brody, L C ^a   Fan, S ^a   Huang, Z ^a   Landers, J P ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL NUCLEUS DNA;

ARTICLE; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; DNA MICROARRAY; GENE AMPLIFICATION; GENE MUTATION; HETERODUPLEX ANALYSIS; HETEROZYGOSITY; HOMOZYGOSITY; HUMAN; HUMAN CELL; LYMPHOBLASTOID CELL; MUTATION RATE; ONCOGENE;

EID: 0035138104     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/47.2.173     Document Type: Article

References (35)

1. Breast cancer susceptibility genes. BRCA1 and BRCA2
2. BRCA1 and BRCA2: From molecular genetics to clinical medicine
3. Mutational spectra of PTEN/MMAC1 gene: A tumor suppressor with lipid phosphatase activity
4. Molecular epidemiology of human cancer: Contribution of mutation spectra studies of tumor suppressor genes
5. Colorectal cancer: Molecules and populations
6. Molecular and genetic targets in early detection
7. The risk of cancer associated with specific mutations of BRCA1 and BRCA2 among Ashkenazi Jews
8. Apolipoprotein E (APOE) allele distribution in the world. Is APOE*4 a 'thrifty' allele?
9. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction
10. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
11. Single-strand conformation polymorphism and heteroduplex analysis for ge·based mutation detection
12. Rapid detection of deletion, insertion and substitution mutations via heteroduplex analysis using capillary- and microchip-based electrophoresis
13. A novel method for detecting point mutations or polymorphisms and its application to population screening for carriers of phenylketonuria
14. PCR amplification of specific alleles (PASA) is a general method for rapidly detecting known single-base changes
15. PCR amplification of specific alleles: Rapid detection of known mutations and polymorphisms
16. A PCR artifact: Generation of heteroduplexes
17. Capillary electrophoresis as a clinical tool for the analysis of protein in serum and other body fluids
18. DNA sequencing by capillary array electrophoresis and microfabricated array systems
19. Simultaneous genetic typing from multiple short tandem repeat loci using a 96-capillary array electrophoresis system
20. High-speed DNA genotyping using microfabricated capillary array electrophoresis chips
21. Optimization of high-speed DNA sequencing on microfabricated capillary electrophoresis channels
22. Radial capillary array electrophoresis microplate and scanner for high-performance nucleic acid analysis
23. Evaluation of silica resins for direct and efficient extraction of DNA from complex biological matrices in a miniaturized format
24. BRCA1 as a potential human prostate tumor suppressor: Modulation of proliferation, damage responses and expression of cell regulatory proteins
25. Characterization of PicoGreen reagent and development of a fluorescence-based solution assay for double-stranded DNA quantitation
26. Confirmation of BRCA1 by analysis of germline mutations linked to breast and ovarian cancer in ten families
27. Somatic mutation of PTEN in bladder carcinoma
28. Molecular diagnostics on microfabricated electrophoretic devices: From slab gel to capillary to microchip-based assays for T- and B-cell lymphoproliferative disorders
29. Rapid diagnosis of herpes simplex encephalitis using microchip electrophoresis of PCR products
30. Identification of a candidate tumor suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers
31. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer
32. The role of MMAC1 mutations in early-onset breast cancer: Causative in association with Cowden syndrome and excluded in BRCA1-negative cases
33. Semi-automated detection of the factor V mutation by allele specific amplification and Capillary electrophoresis
34. Energy-transfer fluorescent reagents for DNA analyses