Sequential analysis of RNA synthesis by microchip electrophoresis

Analytical Biochemistry

Volumn 388, Issue 1, 2009, Pages 161-163

  Umemoto, Yoshihiro ^a   Kataoka, Masatoshi ^b   Yatsushiro, Shouki ^b   Watanabe, Masahiro ^b   Kido, Jun ichi ^a   Kakuhata, Rei ^a   Yamamoto, Takenori ^a   Shinohara, Yasuo ^a   Baba, Yoshinobu ^b,c  

^a UNIVERSITY OF TOKUSHIMA   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^c NAGOYA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ELECTROPHORESIS; RNA;

CONVENTIONAL METHODS; FLUORESCENCE INTENSITIES; HITACHI; IN-VITRO; MICROCHIP ELECTROPHORESIS; RNA SYNTHESIS; SEQUENTIAL ANALYSIS; TIME DEPENDENT;

MICROPROCESSOR CHIPS;

FATTY ACID BINDING PROTEIN 4; RNA;

ACCURACY; ARTICLE; CONTROLLED STUDY; DNA TEMPLATE; FLUORESCENCE; IN VITRO STUDY; MICROCHIP ELECTROPHORESIS; NONHUMAN; PRIORITY JOURNAL; REPRODUCIBILITY; RNA ANALYSIS; RNA SYNTHESIS; SEQUENTIAL ANALYSIS;

EID: 63149141295     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.01.040     Document Type: Article

References (10)

1. Stepien G., Torroni A., Chung A.B., Hodge J.A., and Wallace D.C. Differential expression of adenine nucleotide translocator isoforms in mammalian tissues and during muscle cell differentiation. J. Biol. Chem. 267 (1992) 14592-14597
2. Endo Y., and Sawasaki T. Cell-free expression systems for eukaryotic protein production. Curr. Opin. Biotechnol. 17 (2006) 373-380
3. Hino M., Kataoka M., Kajimoto K., Yamamoto T., Kido J., Shinohara Y., and Baba Y. Efficiency of cell-free protein synthesis based on crude cell extracts from Escherichia coli, wheat germ, and rabbit reticulocytes. J. Biotechnol. 133 (2008) 183-189
4. Zhang L., Dang F., and Baba Y. Microchip electrophoresis-based separation of DNA. J. Pharm. Biomed. Anal. 30 (2003) 1645-1654
5. Hawtin P., Hardern I., Wittig R., Mollenhauer J., Poustka A., Salowsky R., Wulff T., Rizzo C., and Willson B. Utility of lab-on-a-chip technology for high-throughput nucleic acid and protein analysis. Electrophoresis 26 (2005) 3674-3681
6. Schroeder A., Mueller O., Stocker S., Salowsky R., Leiber M., Gassmann M., Lightfoot S., Menzel W., Granzow M., and Ragg T. The RIN: an RNA integrity number for assigning integrity values to RNA measurements. BMC Mol. Biol. 7 (2006) 3
7. Sodowich B.I., Fadl I., and Burns C. Method validation of in vitro RNA transcript analysis on the Agilent 2100 Bioanalyzer. Electrophoresis 28 (2007) 2368-2378
8. Kataoka M., Inoue S., Kajimoto K., Shinohara Y., and Baba Y. Usefulness of microchip electrophoresis for reliable analyses of nonstandard DNA samples and subsequent on-chip enzymatic digestion. Eur. J. Biochem. 271 (2004) 2241-2247
9. Hino M., Shinohara Y., Kajimoto K., Terada H., and Baba Y. Requirement of continuous transcription for the synthesis of sufficient amounts of protein by a cell-free rapid translation system. Protein Expr. Purif. 24 (2002) 255-259
10. Shinohara Y., Yamamoto K., Inoue K., Yamazaki N., and Terada T. Quantitative determinations of the steady state transcript levels of hexokinase isozymes and glucose transporter isoforms in normal rat tissues and the malignant tumor cell line AH130. Biochim. Biophys. 1368 (1998) 129-136