Structural and biochemical insights into 2′-O-methylation at the 3′-terminal nucleotide of RNA by Hen1

Proceedings of the National Academy of Sciences of the United States of America

Volumn 106, Issue 42, 2009, Pages 17699-17704

  Chio, Mui Chan ^a   Zhou, Chun ^a   Brunzelle, Joseph S ^b   Huang, Raven H ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b ARGONNE NATIONAL LABORATORY   (United States)

Author keywords

RNA interference; RNA methylation; RNA repair; X ray crystallography

Indexed keywords

BACTERIAL PROTEIN; DNA; HEN1 PROTEIN; METHYLTRANSFERASE; NUCLEOTIDE; SINGLE STRANDED RNA; UNCLASSIFIED DRUG;

ANABAENA VARIABILIS; ARTICLE; BIOCHEMISTRY; CATALYSIS; CLOSTRIDIUM THERMOCELLUM; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME SPECIFICITY; EUKARYOTE; INTROSPECTION; METHYLATION; MODEL; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN MOTIF; PROTEIN STRUCTURE;

AMINO ACID SEQUENCE; ANABAENA VARIABILIS; ANIMALS; BASE SEQUENCE; BINDING SITES; CATALYTIC DOMAIN; CLOSTRIDIUM THERMOCELLUM; CRYSTALLOGRAPHY, X-RAY; HUMANS; METHYLATION; METHYLTRANSFERASES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NUCLEIC ACID CONFORMATION; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; RNA; SEQUENCE HOMOLOGY, AMINO ACID;

ANABAENA VARIABILIS; ANIMALIA; BACTERIA (MICROORGANISMS); CLOSTRIDIUM THERMOCELLUM; EUKARYOTA;

EID: 70449566825     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0907540106     Document Type: Article

References (28)

1. Fire A, et al. (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391:806-811. (Pubitemid 28099681)
2. Hammond SM, Bernstein E, Beach D, Hannon GJ (2000) An RNA-directed nuclease mediates posttranscriptional gene silencing in Drosophila cells. Nature 404:293-296. (Pubitemid 30163547)
3. Hammond SM, Boettcher S, Caudy AA, Kobayashi R, Hannon GJ (2001) Argonaute2, a link between genetic and biochemical analyses of RNAi. Science 293:1146-1150. (Pubitemid 32758097)
4. Nykanen A, Haley B, Zamore PD (2001) ATP requirements and small interfering RNA structure in the RNA interference pathway. Cell 107:309-321. (Pubitemid 33049975)
5. Yu B, et al. (2005) Methylation as a crucial step in plant microRNA biogenesis. Science 307:932-935. (Pubitemid 40229231)
6. Vagin VV, et al. (2006) A distinct small RNA pathway silences selfish genetic elements in the germ line. Science 313:320-324.
7. Saito K, et al. (2007) Pimet, the Drosophila homolog of HEN1, mediates 2′-O-methylation of Piwi- interacting RNAs at their 3′ ends. Genes Dev 21:1603-1608. (Pubitemid 47026364)
8. Horwich MD, et al. (2007) The Drosophila RNA methyltransferase, DmHen1, modifies germ-line piRNAs and single-stranded siRNAs in RISC. Curr Biol 17:1265-1272.
9. Li J, Yang Z, Yu B, Liu J, Chen X (2005) Methylation protects miRNAs and siRNAs from a 3′-end uridylation activity in Arabidopsis. Curr Biol 15:1501-1507. (Pubitemid 41187490)
10. Shen B, Goodman HM (2004) Uridine addition after microRNA-directed cleavage. Science 306:997.
11. Kurth HM, Mochizuki K (2009) 2′-O-methylation stabilizes Piwi-associated small RNAs and ensures DNA elimination in Tetrahymena. RNA 15:675-685.
12. Yang Z, Ebright YW, Yu B, Chen X (2006) HEN1 recognizes 21-24-nt small RNA duplexes and deposits amethyl group onto the 2′OH of the 3′ terminal nucleotide. Nucleic Acids Res 34:667-675. (Pubitemid 43240253)
13. Macrae IJ, et al. (2006) Structural basis for double-stranded RNA processing by Dicer. Science 311:195-198. (Pubitemid 43108178)
14. Chan CM, Zhou C, Huang RH (2009) Reconstituting a bacterial RNA repair and modification system in vitro. Science, in press.
15. Du Z, Lee JK, Tjhen R, Stroud RM, James TL (2008) Structural and biochemical insights into the dicing mechanism of mouse Dicer: A conserved lysine is critical for dsRNA cleavage. Proc Natl Acad Sci USA 105:2391-2396.
16. Song JJ, Smith SK, Hannon GJ, Joshua-Tor L (2004) Crystal structure of Argonaute and its implications for RISC slicer activity. Science 305:1434-1437. (Pubitemid 39167656)
17. Yuan YR, et al. (2005) Crystal structure of A. aeolicus argonaute, a site-specific DNA-guided endoribonuclease, provides insights into RISC-mediated mRNA cleavage. Mol Cell 19:405-419.
18. Ma JB, et al. (2005) Structural basis for 5′-end-specific recognition of guide RNA by the A. fulgidus Piwi protein. Nature 434:666-670.
19. Parker JS, Roe SM, Barford D (2005) Structural insights into mRNA recognition from a PIWI domain-siRNA guide complex. Nature 434:663-666. (Pubitemid 40488560)
20. Wang Y, Sheng G, Juranek S, Tuschl T, Patel DJ (2008) Structure of the guide-strand-containing argonaute silencing complex. Nature 456:209-213.
21. Wang Y, et al. (2008) Structure of an argonaute silencing complex with a seed-containing guide DNA and target RNA duplex. Nature 456:921-926.
22. Parker JS, Parizotto EA, Wang M, Roe SM, Barford D (2009) Enhancement of the seed-target recognition step in RNA silencing by a PIWI/MID domain protein. Mol Cell 33:204-214.
23. Schubert HL, Blumenthal RM, Cheng X (2003) Many paths to methyltransfer: A chronicle of convergence. Trends Biochem Sci 28:329-335.
24. Martin JL, McMillan FM (2002) SAM (dependent) I AM: The S-adenosylmethionine-dependent methyltransferase fold. Curr Opin Struct Biol 12:783-793.
25. Holm L, Sander C (1993) Protein structure comparison by alignment of distance matrices. J Mol Biol 233:123-138. (Pubitemid 23288916)
26. Tkaczuk KL, Obarska A, Bujnicki JM (2006) Molecular phylogenetics and comparative modeling of HEN1, a methyltransferase involved in plant microRNA biogenesis. BMC Evol Biol 6:6. (Pubitemid 44971315)
27. Emsley P, Cowtan K (2004) Coot: Model-building tools for molecular graphics. Acta Crystallogr D 60:2126-2132. (Pubitemid 41742764)
28. DeLano WL (2002) The PyMOL Molecular Graphic System (DeLano Scientific, San Carlos, CA).