Metabolomics for biotransformations: Intracellular redox cofactor analysis and enzyme kinetics offer insight into whole cell processes

Biotechnology and Bioengineering

Volumn 104, Issue 2, 2009, Pages 251-260

  Schroer, Kirsten ^a,c   Zelic, Bruno ^b   Oldiges, Marco ^a   Lütz, Stephan ^a,c  

^a FORSCHUNGSZENTRUM JÜLICH   (Germany)

^b UNIVERSITY OF ZAGREB   (Croatia)

^c NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Alcohol dehydrogenase; Cofactor pool analysis; Cofactor regeneration; Enzyme kinetics; LC MS MS; Whole cell biotransformation

Indexed keywords

ALCOHOL DEHYDROGENASE; COFACTOR POOL ANALYSIS; COFACTOR REGENERATION; LC-MS/MS; WHOLE CELL BIOTRANSFORMATION;

AMIDES; BIOCONVERSION; CELL MEMBRANES; ENZYME KINETICS; ESCHERICHIA COLI; ORGANIC POLYMERS; RATE CONSTANTS; REDOX REACTIONS; SENSITIVITY ANALYSIS;

ENZYMES;

2 PROPANOL; 3 HYDROXYBUTYRIC ACID; ACETOACETIC ACID DERIVATIVE; ALCOHOL DEHYDROGENASE; CARBENICILLIN; GLUCOSE; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ARTICLE; BACTERIAL CELL; BACTERIUM CULTURE; BIOCATALYST; BIOTRANSFORMATION; CELL TRANSFORMATION; CHEMICAL REACTION; CONTINUOUS STIRRED TANK REACTOR; CULTURE MEDIUM; ENZYME KINETICS; ESCHERICHIA COLI; GAS CHROMATOGRAPHY; IN VITRO STUDY; LACTOBACILLUS BREVIS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MATHEMATICAL MODEL; MEASUREMENT; METABOLITE; METABOLOMICS; NONHUMAN; OXIDATION REDUCTION REACTION; PREDICTION; PROCESS DEVELOPMENT; PROCESS MODEL; RELIABILITY; WHOLE CELL;

2-PROPANOL; ACETOACETATES; ALCOHOL DEHYDROGENASE; BIOTRANSFORMATION; CHROMATOGRAPHY, LIQUID; COENZYMES; ENZYMES; ESCHERICHIA COLI; KINETICS; LACTOBACILLUS BREVIS; METABOLOMICS; NAD; NADP; OXIDATION-REDUCTION; RECOMBINANT PROTEINS; TANDEM MASS SPECTROMETRY;

ESCHERICHIA COLI; LACTOBACILLUS BREVIS;

EID: 70349338910     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22390     Document Type: Article

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