Real-time fret PCR assay for Salmonella enterica serotype detection in food

Military Medicine

Volumn 174, Issue 9, 2009, Pages 983-990

  Olson, Eric V ^a   Gibbins, Carl S ^a   Grayson, J Kevin ^a  

^a DAVID GRANT MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA;

ANIMAL; ARTICLE; FLUORESCENCE RESONANCE ENERGY TRANSFER; FOOD CONTROL; GENETICS; ISOLATION AND PURIFICATION; MEAT; METHODOLOGY; MICROBIOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SALMONELLA ENTERICA; SALMONELLA FOOD POISONING; SENSITIVITY AND SPECIFICITY; SEROTYPING; VEGETABLE;

ANIMALS; DNA, BACTERIAL; FLUORESCENCE RESONANCE ENERGY TRANSFER; FOOD MICROBIOLOGY; MEAT; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SALMONELLA ENTERICA; SALMONELLA FOOD POISONING; SENSITIVITY AND SPECIFICITY; SEROTYPING; VEGETABLES;

EID: 70350743135     PISSN: 00264075     EISSN: None     Source Type: Journal    
DOI: 10.7205/milmed-d-00-9409     Document Type: Article

References (21)

1. Preliminary FoodNet data on the incidence of infection with pathogens transmitted commonly through food: 10 stales 2006. MMWR Morb Mortal Wkly Rep 2006;56:336-9.
2. Patel JR, Bhagwat AA, Sangley GC, Soloman MB: Rapid detection of Salmonella from hydrodynamic pressure-treated poultry using molecular beacon real-time PCR. Food Microbiol 2006; 23: 39-46.
3. Settanni L, Corsetti A: The use of multiplex PCR to detect and differentiate food-and beverage-associated microorganisms: a review. J Microbiol Methods 2007;69:1-22.
4. Carinato ME, Collin-Osdoby P, Yang X, Knox TM, Conlin CA, Miller CG: The apeE gene of Salmonella typhimwium encodes an outer membrane esterase not present in Escherichia coll. J Bacteriol 1998;180:3517-21.
5. Roche Diagnostics Mag NA: Pure LC DNA Isolation Kit III (bacteria, fungi). Available at http://www.roche-applied-science.com/pack-insert/3264785a. pdf; accessed March 2. 2009.
6. Fey A, Eichler S, Flavier S, Christen R, Höfle MG, Guzmán CA: Establishment of real-time PCR-based approach for quantification of bacterial RNA targets in water, using Salmonella as a model organism. Appl Environ Microbiol 2004;70:3618-23.
7. Bohaychuk VM, Gensler GE, McFall ME, King RK, Renter DG: A realtime PCR assay for the detection of Salmonella in a wide variety of food and food-animal matrices. J Food Prot 2007;70:1080-7.
8. Perelle S, Dilasser F, Malomcy B, Grout J, Hoorfar J, Each P: Comparison of PCR-Elisa and Lightcycler real-time PCR assays for detecting Salmonella spp. in milk and meat samples. Mol Cell Probes 2004;18:409-20.
9. Eyigor A, Carli KT: Rapid detection of Salmonella from poultry by realtime polymerase chain reaction fluorescent hybridization probes. Avian Dis 2003; 47: 380-6.
10. Liming SH, Bhagwat AA: Application of a molecular beacon-real time PCR technology to detect Salmonella species contaminating fruits and vegetables. Int J Food Microbiol 2004;95:177-87.
11. Ellingson JL, Anderson JL, Carlson SA, Sharma VK: Twelve hour realtime PCR technique for the sensitive and specific detection of Salmonella in raw and ready-to-eat meat products. Mol Cell Probes 2004; 18: 51-7.
12. Malorny B, Paccassoni E, Each P, Bunge C, Martin A, Helmuth R: Diagnostic real-time PCR for detection of Salmonella in food. Appl Environ Microbiol 2004; 70: 7046-52.
13. Myint MS, Johnson YJ, Tablante NL, Heckerl RA: The effect of preenrichment protocol on the sensitivity and specificity of PCR for detection of naturally contaminated Salmonella in raw poultry compared to conventional culture. Food Microbiol 2006; 23: 599-604.
14. Cheung P-Y, Chan CW, Wong W, Cheung TL, Kam KM: Evaluation of two real-time polymerase chain reaction pathogen detection kits for Salmonella spp. in food. Lett Appl Microbiol 2004;39:509-15.
15. Hein I, Flekna G, Krassnig M, Wagner M: Real-time PCR for the detection of Salmonella spp. in food: an alternative approach to a conventional PCR system suggested by the EGGD-PCR project. J Microbiol Methods 2006;66:538-47.
16. Josefsen MH, Krause M, Hansen F, Hoorfar J: Optimization of a 12-hour taqman-based method for detection of Salmonella bacteria in meat. Appl Environ Microbiol 2007;73:3040-8.
17. Jothikumar N, Wang X, Griffiths MW: Real-time multiplex SYBR green I-based PCR assay for simultaneous detection of Salmonella scrovars and Listeria monocytogenes. J Food Prot 2003;66:2141-5.
18. Malorny B, Bunge C, Helmuth R: A real-time PCR for the detection of Salmonella Enteritidis in poultry meat and consumption eggs. J Microbiol Methods 2007; 70: 245-51.
19. Reynisson E, Josefsen MH, Krause M, Hoorfar J: Evaluation of probe chemistries and platforms to improve the detection limit of real-lime PCR. J Microbiol Methods 2006;66:206-16.
20. Seo KH, Valentin-Bon IE, Brackett RE: Detection and enumeration of Salmonella Enteritidis in homemade ice cream associated with an outbreak: comparison of conventional and real-time PCR methods. J Food Prot 2006; 69: 639-43.
21. Dela Cruz WP, Gozurn MMA, Lineberry SF, et al: Rapid detection of enterotoxigenic Clostridium perfringens by real-time fluorescence resonance energy transfer PCR. J Food Prot 2006;69:1347-53.