Induction of apoptosis by lupeol in human epidermoid carcinoma A431 cells through regulation of mitochondrial, Akt/PKB and NFκB signaling pathways

Cancer Biology and Therapy

Volumn 8, Issue 17, 2009, Pages 1632-1639

  Prasad, Sahdeo ^a   Madan, Esha ^a   Nigam, Nidhi ^a   Roy, Preeti ^a   George, Jasmine ^a   Shukla, Yogeshwer ^a  

^a CSIR INDIAN INSTITUTE OF TOXICOLOGY RESEARCH   (India)

Author keywords

A431 cells; Akt; Apoptosis; Lupeol; NF B; Skin cancer

Indexed keywords

APOPTOTIC PROTEASE ACTIVATING FACTOR 1; BETA ACTIN; CASPASE 3; CASPASE 9; I KAPPA B ALPHA; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; LUPEOL; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; PROTEIN BAD; PROTEIN BAX; PROTEIN BCL 2; PROTEIN KINASE B; REACTIVE OXYGEN METABOLITE; SERINE;

ANTINEOPLASTIC ACTIVITY; APOPTOSIS; ARTICLE; CARCINOMA CELL; CELL CYCLE G1 PHASE; CELL CYCLE M PHASE; CELL CYCLE S PHASE; CELL DEATH; CELL NUCLEUS; CELL PROLIFERATION; CELL SURVIVAL; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOSOL; DRUG EFFECT; DRUG EFFICACY; ENZYME ACTIVATION; HUMAN; HUMAN CELL; IC 50; MITOCHONDRIAL MEMBRANE POTENTIAL; MITOCHONDRION; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; SKIN CARCINOMA; SQUAMOUS CELL CARCINOMA; UPREGULATION; WESTERN BLOTTING;

ANTI-INFLAMMATORY AGENTS; APOPTOSIS; BLOTTING, WESTERN; CARCINOMA, SQUAMOUS CELL; CELL CYCLE; CELL GROWTH PROCESSES; CELL LINE, TUMOR; CELL SURVIVAL; FLOW CYTOMETRY; HUMANS; MITOCHONDRIA; NF-KAPPA B; PENTACYCLIC TRITERPENES; PROTO-ONCOGENE PROTEINS C-AKT; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION;

EID: 70350317603     PISSN: 15384047     EISSN: 15558576     Source Type: Journal    
DOI: 10.4161/cbt.8.17.9204     Document Type: Article

References (45)

1. Deo SV, Hazarika S, Shukla NK, Kumar S, Kar M, Samaiya A. Surgical management of skin cancers: Experience from a regional cancer centre in North India. Indian J Cancer 2005; 42:145-150
2. Jemal A, Siegel R, Ward E, Murray T, Xu J, Thun MJ. Cancer statistics, 2007. CA Cancer J Clin 2007; 57:43-46
3. Bowden GT. Prevention of non-melanoma skin cancer by targeting ultraviolet-B-light signaling. Nat Rev Cancer 2004; 4:23-35.
4. Fournet A, Angelo A, Munoz V, Roblot F, Hocquemiller R, Cave A. Biological and chemical studies of Pera benensis, a Bolivian plant used in folk medicine as a treatment of cutaneous leishmaniasis. J Ethnopharmacol 1992; 37:159-164
5. Kakuda R, Iijima T, Yaoita Y, Machida K, Kikuchi M. Triterpenoids from Gentiana scabra. Photochem 2002; 59:791-794
6. Prasad S, Kalra N, Shukla Y. Hepatoprotective effects of lupeol and mango pulp extract of carcinogen induced alteration in Swiss albino mice. Mol Nutr Food Res 2007; 51:352-359 (Pubitemid 47083670)
7. Geetha T, Varalakshmi P. Anti-inflammatory activity of lupeol and lupeol linoleate in rats. J Ethnopharmacol 2001; 76:77-80. (Pubitemid 32510734)
8. Nigam N, Prasad S, Shukla Y. Preventive effects of lupeol on DMBA induced DNA alkylation damage in mouse skin. Food Chem Toxicol 2007; 45:2331-2335 (Pubitemid 47451582)
9. Latha RM, Lenin M, Rasool M, Varalakshmi P. A novel derivative pentacyclic triterpene and omega 3 fatty acid. Prostaglandins Leukot Essent Fatty Acids 2001; 64:81-85
10. Ziegler HL, Staalso T, Jaroszewski JW. Loading of erythrocyte membrane with pentacyclic triterpenes inhibits Plasmodium falciparum invasion. Planta Med 2006; 72:640-642
11. Saleem M, Afaq F, Adhami VM, Mukhtar H. Lupeol modulates NFkappaB and PI3K/Akt pathways and inhibits skin cancer in CD-1 mice. Oncogene 2004; 23:5203-5214 (Pubitemid 38980353)
12. Prasad S, Nigam N, Kalra N, Shukla Y. Regulation of signaling pathways involved in lupeol induced inhibition of proliferation and induction of apoptosis in human prostate cancer cells. Mol Carcinog 2008; 47:916-924
13. Saleem M, Kaur S, Kweon MH, Adhami VM, Afaq F, Mukhtar H. Lupeol, a fruit and vegetable based triterpene, induces apoptotic death of human pancreatic adenocarcinoma cells via inhibition of Ras signaling pathway. Carcinogenesis 2005; 26:1956-1964 (Pubitemid 41631270)
14. Rodriguez-Hernandez A, Brea-Calvo G, Fernandez-Ayala DJ, Cordero M, Navas P, Sanchez-Alcazar JA. Nuclear caspase-3 and caspase-7 activation, and poly(ADP-ribose) polymerase cleavage are early events in camptothecin-induced apoptosis. Apoptosis 2006; 11:131-139 (Pubitemid 43151651)
15. Kumar A, Takada Y, Boriek AM, Aggarwal BB. Nuclear factor kappaB: its role in health and disease. J Mol Med 2004; 82:434-448 (Pubitemid 39044283)
16. Xu S, Bayat H, Hou X, Jiang B. Ribosomal S6 kinase-1 modulates interleukin-1β-induced persistent activation of NFκB through phosphorylation of IκBβ. Am J Physiol Cell Physiol 2006; 291:1336-1345 (Pubitemid 44878605)
17. Hwang YS, Hodge JC, Sivapurapu N, Lindholm PF. Lysophosphatidic acid stimulates PC3 prostrate cancer cell Matrigel invasion through activation of RhoA and NFkappaB activity. Mol Carcinogenesis 2006; 45:518-529
18. Heber D. Vegetables, fruits and phytoestrogens in the prevention of diseases. J Postgrad Med 2004; 50:145-149 (Pubitemid 39069773)
19. Kris-Etherton PM, Hecker KD, Bonanome A, Coval SM, Binkoski AE, Hilpert KF, et al. Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. Am J Med 2002; 113:71-88. (Pubitemid 36151834)
20. Tanaka R, Kinouchi Y, Wada S, Tokuda H. Potential anti-tumor promoting activity of lupane-type triterpenoids from the stem bark of Glochidion zeylanicum and Phyllanthus flexuosus. Planta Med 2004; 70:1234-1246 (Pubitemid 40101110)
21. Aratanechemuge Y, Hibasami H, Sanpin K, Katsuzaki H, Imai K, Komiya T. Induction of apoptosis by lupeol isolated from mokumen (Gossampinus malabarica L. Merr) in human promyelotic leukemia HL-60 cells. Oncol Rep 2004; 11:289-292
22. Hata K, Hori K, Takahashi S. Differentiation- and apoptosis-inducing activities by pentacyclic triterpenes on a mouse melanoma cell line. J Nat Prod 2002; 65:645-648 (Pubitemid 34547402)
23. Simon HU, Haj-Yehia A, Levi-Schaffer F. Role of reactive oxygen species (ROS) in apoptosis induction. Apoptosis 2004; 5:415-418
24. Mohan KV, Gunasekaran P, Varalakshmi E, Hara Y, Nagini S. In vitro evaluation of the anticancer effect of lactoferrin and tea polyphenol combination on oral carcinoma cells. Cell Biol Int 2007; 31:599-608.
25. Chung YM, Bae YS, Lee SY. Molecular ordering of ROS production, mitochondrial changes, and caspase activation during sodium salicylate-induced apoptosis. Free Radic Biol Med 2003; 34:434-442
26. Tophkhane C, Yang S, Bales W, Archer L, Osunkoya A, Thor AD, et al. Bcl-2 overexpression sensitizes MCF-7 cells to genistein by multiple mechanisms. Int J Oncol 2007; 31:867-874
27. Cohen GM. Caspases: the executioners of apoptosis. Biochem J 1997; 326:1-16.
28. Kolthur-Seetharam U, Dantzer F, McBurney MW, de Murcia G, Sassone-Corsi P. Control of AIF mediated cell death by the functional interplay of SIRT1 and PARP-1 in response to DNA damage. Cell Cycle 2006; 5:873-877 (Pubitemid 44145583)
29. Chen M, Guerrero AD, Huang L. Caspase-9-induced mitochondrial disruption through cleavage of anti-apoptotic Bcl-2 family members. J Biol Chem 2007; 282:33888-33895 (Pubitemid 350159562)
30. Meeran SM, Katiyar SK. Grape seed proanthocyanidins promote apoptosis in human epidermoid carcinoma A431 cells through alterations in Cdki-Cdk-cyclin cascade, and caspase-3 activation via loss of mitochondrial membrane potential. Exp Dermatol 2007; 16:405-415
31. Datta SR, Brunet A, Greenberg ME. Cellular survival: a play in three Akts. Genes Dev 1999; 13:2905-2927
32. Koh PO. Ethanol exposure suppresses survival kinases activation in adult rat testes. J Vet Med Sci 2007; 69:21-24
33. Masters SC, Yang H, Datta SR, Greenberg ME, Fu H. 14-3-3 inhibits Bad-induced cell death through interaction with serine-136. Mol Pharmacol 2001; 60:1325-1331 (Pubitemid 33144903)
34. Claerhout S, Decraene D, Van Laethem A, Van Kelst S, Agostinis P, Garmyn M. AKT delays the early-activated apoptotic pathway in UVB-irradiated keratinocytes via BAD translocation. J Invest Dermatol 2007; 127:429-438 (Pubitemid 46106923)
35. Sastry KS, Karpova Y, Kulik G. Epidermal growth factor protects prostate cancer cells from apoptosis by inducing BAD phosphorylation via redundant signaling pathways. J Biol Chem 2006; 281:27367-27377 (Pubitemid 44401764)
36. Ozes ON, Mayo LD, Gustin JA, Pfeffer SR, Pfeffer LM, Donner DB. NFκB activation by tumour necrosis factor requires the Akt serine-threonine kinase. Nature 1999; 401:82-85 (Pubitemid 29418447)
37. Romashkova JA, Makarov SS. NFκB is a target of AKT in anti-apoptotic PDGF signalling. Nature 1999; 401:86-90. (Pubitemid 29418448)
38. Kalra N, Bhui K, Roy P, Srivastava S, George J, Prasad S, et al. Regulation of p53, nuclear factor kappaB and cyclooxygenase-2 expression by bromelain through targeting mitogen-activated protein kinase pathway in mouse skin. Toxicol Appl Pharmacol 2008; 226:30-37 (Pubitemid 350235577)
39. Arora A, Seth K, Kalra N, Shukla Y. Modulation of P-glycoprotein-mediated multidrug resistance in K562 leukemic cells by indole-3-carbinol. Toxicol Appl Pharmacol 2005; 202:237-243 (Pubitemid 40164277)
40. Nicoletti I, Migliorati G, Pagliacci MC, Grignani F, Riccardi C. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods 1991; 139:271-279
41. Esposti DM, McLennan H. Mitochondria and cells produce reactive oxygen species in virtual anaerobiosis: relevance to ceramide-induced apoptosis. FEBS Lett 1998; 430:338-342 (Pubitemid 28337940)
42. Bai J, Rodriguez AM, Melendez JA, Cederbaum AI. Overexpression of catalase in cytosolic or mitochondrial compartment protects HepG2 cells against oxidative injury. J Biol Chem 1999; 274:26217-26224 (Pubitemid 129520050)
43. Arora A, Kalra N, Shukla Y. Regulation of p21/ras protein expression by diallyl sulfide in DMBA induced neoplastic changes in mouse skin. Cancer Lett 2006; 242:28-36. (Pubitemid 44485292)
44. Kalra N, Seth K, Prasad S, Singh M, Pant AB, Shukla Y. Theaflavins induced apoptosis of LNCaP cells is mediated through induction of p53, downregulation of NFkappaB and mitogen-activated protein kinases pathways. Life Sci 2007; 80:2137-2146 (Pubitemid 46755334)
45. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193:265-275