In vitro and in vivo RNA interference mediated suppression of Tn-caspase-1 for improved recombinant protein production in High Five™ cell culture with the baculovirus expression vector system

Biotechnology and Bioengineering

Volumn 104, Issue 2, 2009, Pages 390-399

  Hebert, Colin G ^a,b,c   Valdes, James J ^b   Bentley, William E ^a,c  

^a UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

^b EDGEWOOD CHEMICAL BIOLOGICAL CENTER   (United States)

^c UNIVERSITY OF MARYLAND   (United States)

Author keywords

Baculovirus expression vector system; BEVS; High Five ; Metabolic engineering; RNA interference; RNAi

Indexed keywords

BACULOVIRUS EXPRESSION VECTOR SYSTEM; BEVS; METABOLIC ENGINEERING; RNA INTERFERENCE; RNAI;

CELL DEATH; CELL MEMBRANES; EXPERIMENTS; GENES; METABOLISM; NUCLEIC ACIDS; PROTEINS; RNA; TRANSCRIPTION; VECTORS;

CELL CULTURE;

BLASTICIDIN S; COMPLEMENTARY DNA; DACTINOMYCIN; DOUBLE STRANDED RNA; GREEN FLUORESCENT PROTEIN; INTERLEUKIN 1BETA CONVERTING ENZYME; MESSENGER RNA; RECOMBINANT PROTEIN; RNA;

APOPTOSIS; ARTICLE; BACULOVIRUS; BACULOVIRUS EXPRESSION SYSTEM; CELL CULTURE; CELL GROWTH; CELL VIABILITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; EXPRESSION VECTOR; HOST CELL; INVERTED REPEAT; METABOLIC ENGINEERING; NONHUMAN; NUCLEOTIDE SEQUENCE; NUTRIENT; PHENOTYPE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA INTERFERENCE;

ANIMALS; BACULOVIRIDAE; CASPASE 1; CELL COUNT; CELL CULTURE TECHNIQUES; CELL LINE; CELL SURVIVAL; GENES, REPORTER; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; LEPIDOPTERA; RECOMBINANT PROTEINS; RNA INTERFERENCE;

TRICHOPLUSIA NI;

EID: 70349330794     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22411     Document Type: Article

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