Purification of human interleukin-2 fusion protein produced in insect larvae is facilitated by fusion with green fluorescent protein and metal affinity ligand

Biotechnology Progress

Volumn 15, Issue 2, 1999, Pages 283-286

  Cha, Hyung Joon ^a,b   Dalal, Nimish G ^a   Pham, Minh Quan ^a   Bentley, William E ^a  

^a UNIVERSITY OF MARYLAND   (United States)

^b Pohang University of Science and Technology (POSTECH)   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ENTEROPEPTIDASE; GREEN FLUORESCENT PROTEIN; HISTIDINE; INTERLEUKIN 2 FUSION PROTEIN; POLYHEDROSIS VIRUS; PROTEIN PURIFICATION; TRICHOPLUSIA NI;

AFFINITY CHROMATOGRAPHY; BIOTECHNOLOGY; FLUORESCENCE; PURIFICATION; VIRUSES;

IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY (IMAC);

PROTEINS;

ANIMALS; BIOLOGICAL MARKERS; ENTEROPEPTIDASE; GENE EXPRESSION; GREEN FLUORESCENT PROTEINS; HUMANS; INDICATORS AND REAGENTS; INSECTS; INTERLEUKIN-2; LARVA; LUMINESCENT PROTEINS; METALS; RECOMBINANT FUSION PROTEINS;

ANIMALIA; AUTOGRAPHA CALIFORNICA; AUTOGRAPHA CALIFORNICA NUCLEOPOLYHEDROVIRUS; CYPOVIRUS; INSECTA; TRICHOPLUSIA NI; UNIDENTIFIED BACULOVIRUS; UNIDENTIFIED NUCLEAR POLYHEDROSIS VIRUSES;

EID: 0000399310     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp990022t     Document Type: Article

References (19)

1. Becht, H.; Muller, H.; Muller, H. K. Comparative Studies on Structural and Antigenic Properties of Two Serotypes of Infectious Bursal Desease Virus. J. Gen. Virol. 1988, 69, 631-640.
2. Boden, V.; Winzerling, J.; Vijayalakshmi, M.; Parath, J. Rapid One-Step Purification of Goat Immunoglobulins by Immobilized Metal Ion Affinity Chromatography. J. Immunol. Methods 1995, 181, 225-232.
3. Cha, H. J.; Pham, M. Q.; Rao, G.; Bentley, W. E. Expression of Green Fluorescent Protein in Insect Larvae and Its Application for Foreign Protein Production. Biotechnol. Bioeng. 1997, 56, 239-247.
4. Cha, H. J.; Dalal, N. G.; Vakharia, V. N.; Bentley, W. E. Expression of Human Interleukin-2 as a Fusion with Green Fluorescent Protein in Suspended Sf-9 Insect Cells, J. Biotechnol. 1999a, in press.
5. Cha, H. J.; Dalal, N. G.; Pham, M. Q.; Vakharia, V. N.; Bentley, W. E. Insect Larval Expression Process is Optimized by Generating Fusions with Green Fluorescent Protein. 1999b, submitted.
6. Chaga, G.; Widersten, M.; Andersson, L.; Parath, J.; Danielson, U.; Mannervik, B. Engineering of a Metal Coordinating Site into Human Glutathione Transferase M1-1 Based on Immobilized Metal Ion Affinity Chromatography of Homologous Rat Enzymes. Protein Eng. 1994, 7, 1115-1119.
7. Chalfie, M.; Tu, Y.; Euskirchen, G.; Ward, W. W.; Prasher, D. C. Green Fluorescent Protein as a Marker for Gene Expression. Science 1994, 263, 802-805.
8. Crameri, A.; Whitehorn, E. A.; Tate, E.; Stemmer, W. P. C. Improved Green Fluorescent Protein by Molecular Evolution using DNA Shuffling. Nat. Biotechnol. 1996, 14, 315-319.
9. Hale, J.; Beidler, D. Purification of Humanized Murine and Murine Monoclonal Antibodies using Immobilized Metal-Affinity Chromatography. Anal. Biochem. 1994, 222, 29-33.
10. Jacobson, E. L.; Pilano, F.; Smith, K. A. Rational Interleukin-2 Therapy for HIV Positive Individuals: Daily Low Doses Enhance Immune Function without Toxicity. Proc. Natl. Acad. Sci. U.S.A. 1996, 93, 10405-10410.
11. Klapper, M. H. The Independent Distribution of Amino Acid Near Neighbor Pairs into Polypeptides. Biochem. Biophys. Res. Commun. 1977, 78, 1018-1024.
12. 4. Nature 1970, 227, 680-685.
13. Morgan, D. A.; Ruscetti, F.; Giallo, F. W. Selective In Vitro Growth of T Lymphocytes from Normal Human Bone Marrows. Science 1976, 193, 1007-1008.
14. Paciotti, G. F.; Tamarkin, L. Interleukin-2 Differentially Affects the Proliferation of a Hormone-dependent and a Hormone-independent Human Breast Cancer Cell Line in vitro and in vivo. Anticancer Res. 1888, 8, 1233-1239.
15. Prasher, D. C.; Eckenrode, V. K.; Ward, W. W.; Prendergast, F. G.; Cormier, M. J.Primary Structure of the Aequorea victoria Green Fluorescent Protein. Gene 1992, 111, 229-233.
16. Rosenburg, S. A.; Grimm, E. A.; McGrogan, M.; Doyle, M.; Kawasaki, E.; Koghs, K.; Mark, D. F. Lymophokine Activated T Cells in the Treatment of Cancer. Science 1984, 223, 1412-1415.
17. Schmidt, W.; Schweighoffer, T.; Herbst, E.; Maass, G.; Berger, M.; Schilcher, F.; Schaffner, G.; Birnstiel, M. L. Cancer Vaccines: the Interleukin 2 Dosage Effect. Proc. Natl. Acad. Sci. U.S.A. 1995, 92, 4711-4714.
18. Shimomura, O.; Johnson, F. H.; Saiga, Y. Excitation, Purification and Properties of Aequorin, a Bioluminescent Protein from the Luminous Hydromedusan. Aequorea. J. Cell. Comput. Physiol. 1962, 59, 223-227.
19. Wang, M.; Bentley, W. E.; Vakharia, V. N. Purification of a Recombinant Protein Produced in a Baculovirus Expression System by Immobilized Metal Affinity Chromatography. Biotechnol. Bioeng. 1994, 43, 349-356.