RNAi-based tuning of cell cycling in Drosophila S2 cells - Effects on recombinant protein yield

Applied Microbiology and Biotechnology

Volumn 73, Issue 5, 2007, Pages 1128-1135

  March, John C ^a,b   Bentley, William E ^a,b  

^a UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

^b UNIVERSITY OF MARYLAND   (United States)

Author keywords

Ago; Apoptosis; Archipelago; Cell culture; Cell cycle; CycE; Cyclin E; Drosophila; GFP; RNAi; S2; Sl 2

Indexed keywords

BIOSYNTHESIS; FLUORESCENT LAMPS; PARAFFINS; PROTEINS; RNA;

AGO; APOPTOSIS; ARCHIPELAGO; CELL CYCLE; CYCE; CYCLIN E; DROSOPHILA; GFP; RNAI;

CELL CULTURE;

DOUBLE STRANDED RNA; DROSOPHILA PROTEIN; GREEN FLUORESCENT PROTEIN; MESSENGER RNA; RECOMBINANT PROTEIN;

BIOENGINEERING; FLY; PROTEIN; RNA;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL CYCLE; CONTROLLED STUDY; DROSOPHILA MELANOGASTER; GENE SILENCING; GENETIC TRANSFECTION; IN VITRO STUDY; INSECT CELL; NONHUMAN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN SYNTHESIS; RNA INTERFERENCE; RNA TRANSCRIPTION;

ANIMALS; CELL CYCLE; CELL CYCLE PROTEINS; CELL LINE; CYCLIN E; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; GENE EXPRESSION REGULATION; GENE SILENCING; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; RECOMBINANT PROTEINS; RNA INTERFERENCE; RNA, DOUBLE-STRANDED; RNA-INDUCED SILENCING COMPLEX; TRANSFECTION;

EID: 33845786069     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0560-x     Document Type: Article

References (23)

1. Abu-Absi NR, Srienc F (2000) Cell cycle events and cell cycle dependent processes in eukaryotic cells. In: Spier RE (ed) Encyclopedia of cell technology. Wiley-Interscience, New York, pp 320-383
2. Boutros M, Kiger AA, Armknecht S, Kerr K, Hild M, Koch B, Haas SA, Consortium HF, Paro R, Perrimon N (2004) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Science 303(5659):832-835
3. Brumby AM, Zraly CB, Horsfield JA, Secombe J, Saint R, Dingwall AK, Richardson H (2002) Drosophila Cyclin E interacts with components of the Brahma complex. EMBO J 21(13):3377-3389
4. Clemens JC, Worby CA, Simonson-Leff N, Muda M, Maehama T, Hemmings BA, Dixon JE (2000) Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways. Proc Natl Acad Sci USA 97(12):6499-6503
5. Fussenegger M, Bailey JE (1998) Molecular regulation of cell-cycle progression and apoptosis in mammalian cells: implications for biotechnology. Biotechnol Prog 14(6):807-833
6. Fussenegger M, Schlatter S, Datwyler D, Mazur X, Bailey JE (1998) Controlled proliferation by multigene metabolic engineering enhances the productivity of Chinese hamster ovary cells. Nat Biotechnol 16(5):468-472
7. Hacker DL, Bertschinger M, Baldi L, Wurm FM (2004) Reduction of adenovirus E1A mRNA by RNAi results in enhanced recombinant protein expression in transiently transfected HEK293 cells. Gene 341:227-234
8. Ibarra N, Watanabe S, Bi JX, Shuttleworth J, Al-Rubeai M (2003) Modulation of cell cycle for enhancement of antibody productivity in perfusion culture of NS0 cells. Biotechnol Prog 19(1):224-228
9. Jia J, Zhang W, Wang B, Trinko R, Jiang J (2003) The Drosophila Ste20 family kinase dMST functions as a tumor suppressor by restricting cell proliferation and promoting apoptosis. Genes Dev 17(20):2514-2519
10. Jones L, Richardson H, Saint R (2000) Tissue-specific regulation of Cyclin E transcription during Drosophila melanogaster embryogenesis. Development 127(21):4619-4630
11. Kramer SF, Bentley WE (2003) RNA interference as a metabolic engineering tool: potential for in vivo control of protein expression in an insect larval model. Metab Eng 5(3):183-190
12. Lee LA, Orr-Weaver TL (2003) Regulation of cell cycles in Drosophila development: intrinsic and extrinsic cues. Annu Rev Genet 37:545-578
13. Maciorowski Z, Delic J, Padoy E, Klijanienko J, Dubray B, Cosset JM, Dumont J, Magdelenat H, Vielh P (1998) Comparative analysis of apoptosis measured by Hoechst and flow cytometry in non-Hodgkin's lymphomas. Cytometry 32(1):44-50
14. March JC, Bentley WE (2004) Insulin stimulates double-stranded RNA uptake in Drosophila S2 cells. Biotechniques 37(6):890-898
15. Mazur X, Fussenegger M, Renner WA, Bailey JE (1998) Higher productivity of growth-arrested Chinese hamster ovary cells expressing the Cyclin-dependent kinase inhibitor p27. Biotechnol Prog 14(5):705-713
16. Mazur X, Eppenberger HM, Bailey JE, Fussenegger M (1999) A novel autoregulated proliferation - controlled production process using recombinant CHO cells. Biotechnol Bioeng 65(2):144-150
17. Moberg KH, Bell DW, Wahrer DC, Haber DA, Hariharan IK (2001) Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell lines. Nature 413(6853):311-316
18. Qu ZL, MacLellan WR, Weiss JN (2003a) Dynamics of the cell cycle: checkpoints, sizers, and timers. Biophys J 85(6):3600-3611
19. Qu ZL, Weiss JN, MacLellan WR (2003b) Regulation of the mammalian cell cycle: a model of the G(1)-to-S transition. Am J Physiol Cell Physiol 284(2):C349-C364
20. Somasundaram K, Zhang HB, Zeng YX, Houvras Y, Peng Y, Zhang HX, Wu GS, Licht JD, Weber BL, ElDeiry WS (1997) Arrest of the cell cycle by the tumour-suppressor BRCA1 requires the CDK-inhibitor p21(WAF1/CiP1). Nature 389(6647):187-190
21. Suzuki E, Ollis DF (1989) Cell-cycle model for antibody-production kinetics. Biotechnol Bioeng 34(11):1398-1402
22. Suzuki E, Ollis DF (1990) Enhanced antibody-production at slowed growth-rates - experimental demonstration and a simple structured model. Biotechnol Prog 6(3):231-236
23. Watanabe S, Shuttleworth J, Al-Rubeai M (2002) Regulation of cell cycle and productivity in NS0 cells by the over-expression of p21 (CIP1). Biotechnol Bioeng 77(1):1-7