Metabolic engineering of the baculovirus-expression system via inverse "shotgun" genomic analysis and RNA interference (dsRNA) increases product yield and cell longevity

Biotechnology and Bioengineering

Volumn 98, Issue 3, 2007, Pages 645-654

  Eun, Jeong Kim ^a   Kramer, Shannon E ^b   Hebert, Colin G ^a,c,e   Valdes, James J ^c   Bentley, William E ^a,d,e  

^a UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

^b BAYLOR COLLEGE OF DENTISTRY   (United States)

^c EDGEWOOD CHEMICAL BIOLOGICAL CENTER   (United States)

^d UNIVERSITY OF MARYLAND   (United States)

^e UNIVERSITY OF MARYLAND   (United States)

Author keywords

Apoptosis; Baculovirus expression vector systems; Caspase; Cathepsin; dsRNA; Metabolic engineering; RNA interference; Spodoptera frugiperda cells; Trichoplusia ni larvae

Indexed keywords

BACULOVIRUS EXPRESSION VECTOR SYSTEMS; METABOLIC ENGINEERING; SPODOPTERA FRUGIPERDA CELLS; TRICHOPLUSIA NI LARVAE;

CELL DEATH; GENES; HORMONES; PROTEINS; RNA; VIRUSES;

BIOMEDICAL ENGINEERING;

DOUBLE STRANDED RNA; ELONGATION FACTOR 2; GREEN FLUORESCENT PROTEIN; INTERLEUKIN 1BETA CONVERTING ENZYME; JUVENILE HORMONE; PROTEIN DERIVATIVE; PROTEIN P35; RNA; UNCLASSIFIED DRUG; V CATH PROTEIN;

ANIMAL CELL; APOPTOSIS; ARTICLE; BACULOVIRUS; BACULOVIRUS EXPRESSION SYSTEM; CELL CULTURE; EXPRESSION VECTOR; FEMALE; GENE REPRESSION; GENE TARGETING; GENOME ANALYSIS; GROWTH; HOST; IN VITRO STUDY; INJECTION; INSECT CELL; INSECT GENETICS; LARVA; LARVAL DEVELOPMENT; LEPIDOPTERA; LONGEVITY; METABOLIC ENGINEERING; MODEL; NONHUMAN; RNA INTERFERENCE; STATISTICAL SIGNIFICANCE; SYNTHESIS; TRICHOPLUSIA NI;

ANIMALS; BACULOVIRIDAE; CELL AGING; CELL PROLIFERATION; CELL SURVIVAL; CHROMOSOME MAPPING; GENETIC ENHANCEMENT; GENETIC VECTORS; PROMOTER REGIONS (GENETICS); PROTEIN ENGINEERING; RECOMBINANT PROTEINS; RNA INTERFERENCE; SPODOPTERA;

EUKARYOTA; HEXAPODA; SPODOPTERA FRUGIPERDA; TRICHOPLUSIA NI;

EID: 35048855619     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21353     Document Type: Article

References (45)

1. Agrawal N, Malhotra P, Bhatnagar RK. 2004. siRNA-directed silencing of transgene expressed in cultured insect cells. Biochem Biophys Res Commun 320(2):428-434.
2. Ahmad M, Srinivasula SM, Wang L, Litwack G, Alnemri TF, Alnemri ES. 1997. Spodoptera frugiperda caspase-1, a novel insect death protease that cleaves the nuclear immonophilin FKBP46, is the target of the baculovirus antiapoptotic protein p35. J Biol Chem 272:1421-1424.
3. Aparna G, Bhuyan AK, Sahdev S, Hasnain SE, Kaufman RJ, Ramaiah KV. 2003. Stress-induced apoptosis in Spodoptera frugiperda (Sf-9) cells: Baculovirus p35 mitigates eIF2 alpha phosphorylation. Biochemistry 42(51):15352-15360.
4. Bettencourt-Dias M, Giet R, Sinka R, Mazumdar A, Lock WG, Balloux F, Zafiropoulos PJ, Yamaguchi S, Winter S, Carthew RW, Cooper M, Jones D, Frenz L, Glover DM. 2004. Genome-wide survey of protein kinases required for cell cycle progression. Nature 432:980-987.
5. Boutros M, Kiger AA, Armknecht S, Kerr K, Hild M, Koch B, Haas SA, Consortium HF, Paro R, Perrimon N. 2004. Genome-wide RNAi analysis of growth and viability in Drosophila cells. Science 303:832-835.
6. Cha HJ, Dalal NG, Pham MQ, Bentley WE. 1999a. Purification of human interleukin-2 fusion protein produced in insect larvae is facilitated by fusion with green fluorescent protein and metal affinity ligand. Biotechnol Prog 15:283-286.
7. Cha HJ, Dalal NG, Pham MQ, Vakharia VN, Rao G, Bentley WE. 1999b. Insect larval expression process is optimized by generating fusions with green fluorescent protein. Biotechnol Bioeng 65:316-324.
8. Cha HJ, Dalal NG, Vakharia VN, Bentley WE. 1999c. Expression and purification of human interleukin-2 simplified as a fusion with green fluorescent protein in suspended Sf-9 insect cells. J Biotechnol 69: 9-17.
9. Chung JD, Zabel C, Sisnkey AJ, Stephanopoulos G. 1997. Extend of Sp2/0 hybridoma cell viability through interleukin-6 supplementation. Biotechnol Bioeng 55:439-446.
10. Clem RJ, Fechheimer M, Miller LK. 1991. Prevention of apoptosis by a baculovirus gene during infection of insect cells. Science 254:1388-1390.
11. Crook NE, Clem RJ, Miller LK. 1993. An apoptosis-inhibiting baculovirus gene with a zinc finger-like motif. J Virol 67:2168-2174.
12. Desai RP, Papoutsakis ET. 1999. Antisense RNA strategies for metabolic engineering of Clostridium acetobutylicum. Appl Environ Microb 65:936-945.
13. Fire A, Xu SQ, Montgomery MK, Kostas SA, Driver SE, Mello CC. 1998. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391:806-811.
14. Hacker DL, Bertschinger M, Baldi L, Wurm FM. 2004. Reduction of adenovirus E1A mRNA by RNAi results in enhanced recombinant protein expression in transiently transfected HEK293 cells. Gene 341:227-234.
15. Hannon GJ. 2002. RNA interference. Nature 418:244-251.
16. Horn LG, Ohkawa T, Trudeau D, Volkman LE. 2002. Autographa californica M nucleopolyhedrovirus proV-CATH is activated during infected cell death. Virology 296:212-218.
17. Kaba SA, Salcedo AM, Wafula PO, Vlak JM, van Oers MM. 2004. Development of a chitinase and v-cathepsin negative bacmid for improved integrity of secreted recombinant proteins. J Virol Meth 122:113-118.
18. Kim NS, Lee GM. 2002. Inhibition of sodium butyrate-induced apoptosis in recombinant Chinese hamster ovary cells by constitutively expressing antisense RNA of caspase-3. Biotehnol Bioeng 78:217-228.
19. Kramer SF, Bentley WE. 2003. RNA interference as a metabolic engineering tool: Potential fir in vivo control of protein expression in an insect larval model. Metab Eng 5:183-190.
20. Kramer SF, Rostov Y, Rao G, Bentley WE. 2003. Ex vivo monitoring of protein production in baculovirus-infected Trichoplusia ni larvae with a GFP-specific optical probe. Biotechnol Bioeng 83:241-247.
21. LaCount DJ, Hanson SF, Schneider CL, Friesen PD. 2000. Caspase inhibitor P35 and inhibitors of apoptosis Op-IAP block in vitro proteolytic activation of an effector caspase at different steps. J Biol Chem 275:15657-15664.
22. Lin G, Li G, Granados RR, Blissard GW. 2001. Stable cell lines expressing baculovirus p35: Resistance to apoptosis and nutrient stress, and increased glycoprotein secretion. In Vitro Cell Dev Bio Anim 37:293-302.
23. Lin CC, Hsu JT, Huang KL, Tang HK, Lai YK. 2006. Stable RNA interference in Spodoptera frugiperda cells by a DNA vector-based method. Biotechnol Lett 28:271-277.
24. March JC, Bentley WE. 2006. Engineering eukaryotic signal transduction with RNAi: Enhancing Drosophila S2 cell growth and recombinant protein synthesis via silencing of TSC1. Biotechnol Bioeng 95(4):645-652.
25. Mastrangelo AJ, Betenbaugh MJ. 1998. Overcoming apoptosis: New methods for improving protein-expression systems. Trends Biotechnol 16:88-95.
26. Mastrangelo AJ, Hardwick JM, Bex F, Betenbaugh MJ. 2000. Part I. Bcl-2 and Bcl-x(L) limit apoptosis upon infection with alphavirus vectors. Biotechnol Bioeng 67:544-554.
27. Mastrangelo AJ, Hardwick JM, Zou S, Betenbaugh MJ. 2000. Part II. Overexpression of bcl-2 family members enhances survival of mammalian cells in response to various culture insults. Biotechnol Bioeng 67:555-564.
28. Montgomery MK, Fire A. 1998. Double-stranded RNA as a mediator in sequence-specific genetic silencing and co-suppression. Trends Genent 14:255-258.
29. Naggie S, Bentley WE. 1998. Appearance of protease activities coincide with p10 and polyhedron-driven protein production in the baculovirus expression system: Effects on yield. Biotechnol Prog 14:227-232.
30. Ooi BG, Miller LK. 1988. Regulation of host RNA levels during baculovirus infection. Virology 166:515-523.
31. O'Reilly DR, Miller LK, Luckow VA. 1992. Baculovirus expression vectors: A laboratory manual. Freeman, New York.
32. Pham MQ, Naggie S, Wier M, Cha HJ, Bentley WE. 1999. Human interleukin-2 production in insect (Trichoplusia ni) larvae: Effects and partial control of proteolysis. Biotechnol Bioeng 62:175-182.
33. Prati EG, Scheidegger P, Sburlati AR, Bailey JE. 1998. Antisense strategies for glycosylation engineering of Chinese hamster ovary (CHO) cells. Biotechnol Bioeng 59:445-450.
34. Prati EG, Matasci M, Suter TB, Dinter A, Sburlati AR, Bailey JE. 2002. Engineering of coordinated up- and down-regulation of two glycosyltransferases of the O-glycosylation pathway in Chinese hamster ovary (CHO) cells. Biotechnol Bioeng 79:580-585.
35. Richardson CD. editor. 1995. Baculovirus expression protocols. Totowa, NJ: Human Press.
36. Rocheleau CE, Downs WD, Lin RL, Wittmann C, Bei YX, Cha YH, Ali M, Priess JR, Mello CC. 1997. Wnt signaling and APC-related gene specify endoderm in early C. elegans embryos. Cell 90:707-716.
37. Sauerwald TM, Betenbaugh MJ, Oyler GA. 2002. Inhibiting apoptosis in mammalian cell culture using the caspase inhibitor XIAP and deletion mutants. Biotehnol Bioeng 77:704-716.
38. Seshagiri S, Miller LK. 1997. Baculovirus inhibitors of apoptosis (IAP) block activation of Sf-caspase-1. Proc Natl Acad Sci USA 94:13606-13611.
39. Slack JM, Kuzio J, Faulkner P. 1995. Characterization of v-cath, a cathepsin L-like proteinase expressed by the baculovirus Autographa californica multiple nuclear polyhedrosis virus. J Gen Virol 76:1091-1098.
40. Srivastava R, Cha HJ, Peterson MS, Bentley WE. 2000. Antisense downregulation of sigma (32) as a transient metabolic controller in Escherichia coli: Effects on yield of active organophosphorus hydrolase. Appl Environ Microb 66:4366-4371.
41. Sui G, Soohoo C, Affar EB, Gay F, Shi Y, Foreester WC, Shi Y. 2002. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc Natl Acad Sci USA 99:5515-5520.
42. Sung YH, Lee GM. 2005. Enhanced human thrombopoietin production by sodium butyrate addition to serum-free suspension culture of Bcl-2-overexpressing CHO cells. Biotechnol Prog 21:50-57.
43. Sung YH, Hwang SJ, Lee GM. 2005. Influence of down-regulation of caspase-3 by siRNAs on sodium-butyrate-induced apoptotic cell death of Chinese hamster ovary cells producing thrombopoietin. Metab Engin 7:457-466.
44. Timmons L, Fire A. 1998. Specific interference by ingested dsRNA. Nature 395:854.
45. Xia H, Mao Q, Paulson HL, Davidson BL. 2002. siRNA-mediated gene silencing in vitro and in vivo. Nat Biotechol 20:1006-1010.