Simultaneous knockdown of the expression of two genes using multiple shRNAs and subsequent knock-in of their expression

Nature Protocols

Volumn 4, Issue 9, 2009, Pages 1338-1349

  Xu, Xue Ming ^a   Yoo, Min Hyuk ^a   Carlson, Bradley A ^a   Gladyshev, Vadim N ^b   Hatfield, Dolph L ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b UNIVERSITY OF NEBRASKA LINCOLN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

K RAS PROTEIN; SELENOPROTEIN; SHORT HAIRPIN RNA; THIOREDOXIN REDUCTASE 1;

ANIMAL CELL; ARTICLE; CELL FUNCTION; CONTROLLED STUDY; EXPERIMENTAL DESIGN; GENE CONSTRUCT; GENE EXPRESSION; GENE LOSS; GENE MUTATION; GENE TARGETING; GENETIC RECOMBINATION; MOLECULAR CLONING; MOUSE; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN SYNTHESIS;

GENE EXPRESSION; GENE KNOCK-IN TECHNIQUES; GENE KNOCKOUT TECHNIQUES; GENETIC VECTORS; MUTAGENESIS; RNA INTERFERENCE; RNA, SMALL INTERFERING; RNA, UNTRANSLATED; TRANSFECTION;

EID: 70249087660     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2009.145     Document Type: Article

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