Development and validation of vectors containing multiple siRNA expression cassettes for maximizing the efficiency of gene silencing

BMC Biotechnology

Volumn 6, Issue , 2006, Pages

  Wang, Shunqing ^a,b   Shi, Zhenqi ^a   Liu, Wei ^a   Jules, Joel ^a   Feng, Xu ^a  

^a University of Alabama at Birmingham   (United States)

^b GUANGZHOU FIRST PEOPLE'S HOSPITAL   (China)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE-STRANDED RNA (DSRNA); GENE SILENCING; RNA POLYMERASE;

CELL CULTURE; GENETIC ENGINEERING; VECTORS;

RNA;

SMALL INTERFERING RNA;

ANIMAL CELL; ARTICLE; CLONING VECTOR; CONTROLLED STUDY; EXPRESSION VECTOR; GENE CASSETTE; GENE EXPRESSION REGULATION; GENE SILENCING; MOLECULAR CLONING; MOUSE; NONHUMAN; RNA VECTOR; VALIDATION PROCESS;

BASE SEQUENCE; CLONING, MOLECULAR; GENE SILENCING; GENETIC ENGINEERING; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; RNA, SMALL INTERFERING;

EID: 33846906043     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-6-50     Document Type: Article

References (15)

1. Cogoni C Macino G Post-transcriptional gene silencing across kingdoms. [Review] [50 refs] Current Opinion in Genetics & Development 2000, 10:638-643. 10.1016/S0959-437X(00)00134-9 11088014
2. McManus MT Sharp PA Gene silencing in mammals by small interfering RNAs. [Review] [112 refs] Nature Reviews Genetics 2002, 3:737-747. 10.1038/ nrg908 12360232
3. Matzke MA Birchler JA RNAi-mediated pathways in the nucleus. [Review] [130 refs] Nature Reviews Genetics 2005, 6:24-35. 10.1038/nrg1500 15630419
4. Elbashir SM Harborth J Weber K Tuschl T Analysis of gene function in somatic mammalian cells using small interfering RNAs Methods 2002, 26:199-213. 10.1016/S1046-2023(02)00023-3 12054897
5. Elbashir SM Harborth J Lendeckel W Yalcin A Weber K Tuschl T Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells.[see comment] Nature 2001, 411:494-498. 10.1038/35078107 11373684
6. Cullen BR RNA interference: Antiviral defense and genetic tool. [Review] [16 refs] Nature Immunology 2002, 3:597-599. 10.1038/ni0702-597 12087412
7. Sui G Soohoo C Affar EB Gay F Shi Y Forrester WC Shi Y A DNA vector-based RNAi technology to suppress gene expression in mammalian cells Proc Natl Acad Sci U S A 2002, 99:5515-5520. 122801 11960009 10.1073/pnas.082117599
8. Miyagishi M Taira K U6 promoter-driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells.[see comment] Nat Biotechnol 2002, 20:497-500. 10.1038/nbt0502-497 11981564
9. Sui G Shi Y Gene silencing by a DNA vector-based RNAi technology Methods in Molecular Biology 2005, 309:205-218. 15990402
10. Feng X Regulatory roles and molecular signaling of TNF family members in osteoclasts. [Review] [133 refs] Gene 2005, 350:1-13. 10.1016/ j.gene.2005.01.014 15777737
11. Liu W Xu D Yang H Xu H Shi Z Cao X Takeshita S Liu J Teale M Feng X Functional identification of three RANK cytoplasmic motifs mediating osteoclast differentiation and function J Biol Chem 2004, 279:54759-54769 10.1074/jbc.M404687200 15485878
12. Liu W Wang S Wei S Sun L Feng X RANK cytoplasmic motif, PFQEP369-373, plays a predominant role in osteoclast survival in part by activating Akt/PKB and its downstream effector AFX/FOXO4 J Biol Chem 2005, 280:43064-43072. 10.1074/jbc.M509006200 16260781
13. Feng X Novack DV Faccio R Ory DS Aya K Boyer MI McHugh KP Ross FP Teitelbaum SL A Glanzmann's mutation in beta 3 integrin specifically impairs osteoclast function. J Clin Invest 2001, 107:1137-1144. 209281 11342577
14. Takeshita S Kaji K Kudo A Identification and characterization of the new osteoclast progenitor with macrophage phenotypes being able to differentiate into mature osteoclasts J Bone Miner Res 2000, 15:1477-1488. 10.1359/jbmr.2000.15.8.1477 10934646
15. Morita S Kojima T Kitamura T Plat-E: An efficient and stable system for transient packaging of retroviruses Gene Therapy 2000, 7:1063-1066. 10.1038/sj.gt.3301206 10871756