Identification of a testicular odorant receptor mediating human sperm chemotaxis

Science

Volumn 299, Issue 5615, 2003, Pages 2054-2058

  Spehr, Marc ^a   Gisselmann, Günter ^a   Poplawski, Alexandra ^a   Riffell, Jeffrey A ^b   Wetzel, Christian H ^a   Zimmer, Richard K ^b   Hatt, Hanns ^a  

^a RUHR UNIVERSITY BOCHUM   (Germany)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; CELLS; HUMAN ENGINEERING; MEDICAL IMAGING;

OLFACTORY RECEPTORS (OR);

CLONING;

CHEMOATTRACTANT;

BIOLOGY; SPERM;

ARTICLE; BIOASSAY; CALCIUM SIGNALING; CHEMOTAXIS; CONTROLLED STUDY; FERTILIZATION; HUMAN; HUMAN CELL; HUMAN TISSUE; ODOR; OLFACTORY RECEPTOR; PRIORITY JOURNAL; SPERMATOZOON MOTILITY; STRUCTURE ANALYSIS; TESTIS;

ADENYLATE CYCLASE; ALDEHYDES; BINDING SITES; CALCIUM; CALCIUM SIGNALING; CELL LINE; CHEMOTACTIC FACTORS; CHEMOTAXIS; CLONING, MOLECULAR; DOSE-RESPONSE RELATIONSHIP, DRUG; FERTILIZATION; GENE EXPRESSION PROFILING; HUMANS; LIGANDS; MALE; MOLECULAR STRUCTURE; ODORS; PHOSPHOLIPASE C; RECEPTORS, ODORANT; RECOMBINANT FUSION PROTEINS; SEMINAL PLASMA PROTEINS; SPERM MOTILITY; SPERMATOZOA; TESTIS;

EID: 0037470996     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1080376     Document Type: Article

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22. We thank B. W. Ache and C. A. Zimmer for valuable comments that greatly improved earlier drafts of this manuscript; A. Sattler for construction of the vector pOLF-express; A. Stoeck, B. Pohl, P. J. Krug, and H. Bartel for technical assistance; C. Meier for help with digital microscopy; and K. M. Müller and R. Dermietzel for testicular tissue and human sperm samples. Analysis of sperm chemotaxis was supported by NSF grants IBN 01-32635 and IBN 02-06775.