-
1
-
-
0037129827
-
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
-
RESEARCH0034
-
Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, et al. (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3: RESEARCH0034.
-
(2002)
Genome Biol
, vol.3
-
-
Vandesompele, J.1
De Preter, K.2
Pattyn, F.3
Poppe, B.4
Van Roy, N.5
-
2
-
-
0032883806
-
Housekeeping genes as internal standards: Use and limits
-
Thellin O, Zorzi W, Lakaye B, De Borman B, Coumans B, et al. (1999) Housekeeping genes as internal standards: use and limits. J Biotechnol 75: 291-295.
-
(1999)
J Biotechnol
, vol.75
, pp. 291-295
-
-
Thellin, O.1
Zorzi, W.2
Lakaye, B.3
De Borman, B.4
Coumans, B.5
-
3
-
-
0033775615
-
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
-
Bustin SA (2000) Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 25: 169-193.
-
(2000)
J Mol Endocrinol
, vol.25
, pp. 169-193
-
-
Bustin, S.A.1
-
4
-
-
0037109753
-
Quantitative real-time reverse transcription polymerase chain reaction: Normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies
-
Tricarico C, Pinzani P, Bianchi S, Paglierani M, Distante V, et al. (2002) Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies. Anal Biochem 309: 293-300.
-
(2002)
Anal Biochem
, vol.309
, pp. 293-300
-
-
Tricarico, C.1
Pinzani, P.2
Bianchi, S.3
Paglierani, M.4
Distante, V.5
-
5
-
-
12944307847
-
Housekeeping gene variability in normal and cancerous colorectal, pancreatic, esophageal, gastric and hepatic tissues
-
Rubie C, Kempf K, Hans J, Su T, Tilton B, et al. (2005) Housekeeping gene variability in normal and cancerous colorectal, pancreatic, esophageal, gastric and hepatic tissues. Mol Cell Probes 19: 101-109.
-
(2005)
Mol Cell Probes
, vol.19
, pp. 101-109
-
-
Rubie, C.1
Kempf, K.2
Hans, J.3
Su, T.4
Tilton, B.5
-
6
-
-
0034694128
-
Effect of experimental treatment on housekeeping gene expression: Validation by real-time, quantitative RT-PCR
-
Schmittgen TD, Zakrajsek BA (2000) Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR. J Biochem Biophys Methods 46: 69-81.
-
(2000)
J Biochem Biophys Methods
, vol.46
, pp. 69-81
-
-
Schmittgen, T.D.1
Zakrajsek, B.A.2
-
7
-
-
0033575003
-
Direct comparison of GAPDH, beta-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia
-
Zhong H, Simons JW (1999) Direct comparison of GAPDH, beta-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia. Biochem Biophys Res Commun 259: 523-526.
-
(1999)
Biochem Biophys Res Commun
, vol.259
, pp. 523-526
-
-
Zhong, H.1
Simons, J.W.2
-
8
-
-
0035197018
-
Beta-actin-an unsuitable internal control for RT-PCR
-
Selvey S, Thompson EW, Matthaei K, Lea RA, Irving MG, et al. (2001) Beta-actin-an unsuitable internal control for RT-PCR. Mol Cell Probes 15: 307-311.
-
(2001)
Mol Cell Probes
, vol.15
, pp. 307-311
-
-
Selvey, S.1
Thompson, E.W.2
Matthaei, K.3
Lea, R.A.4
Irving, M.G.5
-
9
-
-
0036178995
-
Control genes and variability: Absence of ubiquitous reference transcripts in diverse mammalian expression studies
-
Lee PD, Sladek R, Greenwood CM, Hudson TJ (2002) Control genes and variability: absence of ubiquitous reference transcripts in diverse mammalian expression studies. Genome Res 12: 292-297.
-
(2002)
Genome Res
, vol.12
, pp. 292-297
-
-
Lee, P.D.1
Sladek, R.2
Greenwood, C.M.3
Hudson, T.J.4
-
10
-
-
0035577641
-
Identification and validation of endogenous reference genes for expression profiling of T helper cell differentiation by quantitative real-time RT-PCR
-
Hamalainen HK, Tubman JC, Vikman S, Kyrola T, Ylikoski E, et al. (2001) Identification and validation of endogenous reference genes for expression profiling of T helper cell differentiation by quantitative real-time RT-PCR. Anal Biochem 299: 63-70.
-
(2001)
Anal Biochem
, vol.299
, pp. 63-70
-
-
Hamalainen, H.K.1
Tubman, J.C.2
Vikman, S.3
Kyrola, T.4
Ylikoski, E.5
-
11
-
-
27644563899
-
The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization
-
Dheda K, Huggett JF, Chang JS, Kim LU, Bustin SA, et al. (2005) The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization. Anal Biochem 344: 141-143.
-
(2005)
Anal Biochem
, vol.344
, pp. 141-143
-
-
Dheda, K.1
Huggett, J.F.2
Chang, J.S.3
Kim, L.U.4
Bustin, S.A.5
-
12
-
-
12344270322
-
Normalization of gene expression measurements in tumor tissues: Comparison of 13 endogenous control genes
-
de Kok JB, Roelofs RW, Giesendorf BA, Pennings JL, Waas ET, et al. (2005) Normalization of gene expression measurements in tumor tissues: comparison of 13 endogenous control genes. Lab Invest 85: 154-159.
-
(2005)
Lab Invest
, vol.85
, pp. 154-159
-
-
de Kok, J.B.1
Roelofs, R.W.2
Giesendorf, B.A.3
Pennings, J.L.4
Waas, E.T.5
-
13
-
-
21344471138
-
Real-time RT-PCR normalisation; strategies and considerations
-
Huggett J, Dheda K, Bustin S, Zumla A (2005) Real-time RT-PCR normalisation; strategies and considerations. Genes Immun 6: 279-284.
-
(2005)
Genes Immun
, vol.6
, pp. 279-284
-
-
Huggett, J.1
Dheda, K.2
Bustin, S.3
Zumla, A.4
-
14
-
-
0035423813
-
Ribosomal 18S RNA prevails over glyceraldehyde-3-phosphate dehydrogenase and beta-actin genes as internal standard for quantitative comparison of mRNA levels in invasive and noninvasive human melanoma cell subpopulations
-
Goidin D, Mamessier A, Staquet MJ, Schmitt D, Berthier-Vergnes O (2001) Ribosomal 18S RNA prevails over glyceraldehyde-3-phosphate dehydrogenase and beta-actin genes as internal standard for quantitative comparison of mRNA levels in invasive and noninvasive human melanoma cell subpopulations. Anal Biochem 295: 17-21.
-
(2001)
Anal Biochem
, vol.295
, pp. 17-21
-
-
Goidin, D.1
Mamessier, A.2
Staquet, M.J.3
Schmitt, D.4
Berthier-Vergnes, O.5
-
15
-
-
7444226814
-
Equivalence test in quantitative reverse transcription polymerase chain reaction: Confirmation of reference genes suitable for normalization
-
Haller F, Kulle B, Schwager S, Gunawan B, von Heydebreck A, et al. (2004) Equivalence test in quantitative reverse transcription polymerase chain reaction: confirmation of reference genes suitable for normalization. Anal Biochem 335: 1-9.
-
(2004)
Anal Biochem
, vol.335
, pp. 1-9
-
-
Haller, F.1
Kulle, B.2
Schwager, S.3
Gunawan, B.4
von Heydebreck, A.5
-
16
-
-
28544451081
-
Gene expression studies in prostate cancer tissue: Which reference gene should be selected for normalization?
-
Ohl F, Jung M, Xu C, Stephan C, Rabien A, et al. (2005) Gene expression studies in prostate cancer tissue: which reference gene should be selected for normalization? J Mol Med 83: 1014-1024.
-
(2005)
J Mol Med
, vol.83
, pp. 1014-1024
-
-
Ohl, F.1
Jung, M.2
Xu, C.3
Stephan, C.4
Rabien, A.5
-
17
-
-
0346728757
-
Guideline to reference gene selection for quantitative real-time PCR
-
Radonic A, Thulke S, Mackay IM, Landt O, Siegert W, et al. (2004) Guideline to reference gene selection for quantitative real-time PCR. Biochem Biophys Res Commun 313: 856-862.
-
(2004)
Biochem Biophys Res Commun
, vol.313
, pp. 856-862
-
-
Radonic, A.1
Thulke, S.2
Mackay, I.M.3
Landt, O.4
Siegert, W.5
-
18
-
-
7444225172
-
Reference genes identified in SH-SY5Y cells using custom-made gene arrays with validation by quantitative polymerase chain reaction
-
Hoerndli FJ, Toigo M, Schild A, Gotz J, Day PJ (2004) Reference genes identified in SH-SY5Y cells using custom-made gene arrays with validation by quantitative polymerase chain reaction. Anal Biochem 335: 30-41.
-
(2004)
Anal Biochem
, vol.335
, pp. 30-41
-
-
Hoerndli, F.J.1
Toigo, M.2
Schild, A.3
Gotz, J.4
Day, P.J.5
-
19
-
-
32644481938
-
Genomewide identification and testing of superior reference genes for transcript normalization in Arabidopsis
-
Czechowski T, Stitt M, Altmann T, Udvardi MK, Scheible WR (2005) Genomewide identification and testing of superior reference genes for transcript normalization in Arabidopsis. Plant Physiol 139: 5-17.
-
(2005)
Plant Physiol
, vol.139
, pp. 5-17
-
-
Czechowski, T.1
Stitt, M.2
Altmann, T.3
Udvardi, M.K.4
Scheible, W.R.5
-
20
-
-
9144224425
-
Selection and validation of endogenous reference genes using a high throughput approach
-
Jin P, Zhao Y, Ngalame Y, Panelli MC, Nagorsen D, et al. (2004) Selection and validation of endogenous reference genes using a high throughput approach. BMC Genomics 5: 55.
-
(2004)
BMC Genomics
, vol.5
, pp. 55
-
-
Jin, P.1
Zhao, Y.2
Ngalame, Y.3
Panelli, M.C.4
Nagorsen, D.5
-
21
-
-
25844491646
-
Selection of control genes for quantitative RT-PCR based on microarray data
-
Shulzhenko N, Yambartsev A, Goncalves-Primo A, Gerbase-DeLima M, Morgun A (2005) Selection of control genes for quantitative RT-PCR based on microarray data. Biochem Biophys Res Commun 337: 306-312.
-
(2005)
Biochem Biophys Res Commun
, vol.337
, pp. 306-312
-
-
Shulzhenko, N.1
Yambartsev, A.2
Goncalves-Primo, A.3
Gerbase-DeLima, M.4
Morgun, A.5
-
23
-
-
12344275541
-
Limited agreement among three global gene expression methods highlights the requirement for non-global validation
-
Haverty PM, Hsiao LL, Gullans SR, Hansen U, Weng Z (2004) Limited agreement among three global gene expression methods highlights the requirement for non-global validation. Bioinformatics 20: 3431-3441.
-
(2004)
Bioinformatics
, vol.20
, pp. 3431-3441
-
-
Haverty, P.M.1
Hsiao, L.L.2
Gullans, S.R.3
Hansen, U.4
Weng, Z.5
-
24
-
-
25444531992
-
Evaluation of the similarity of gene expression data estimated with SAGE and Affymetrix GeneChips
-
van Ruissen F, Ruijter JM, Schaaf GJ, Asgharnegad L, Zwijnenburg DA, et al. (2005) Evaluation of the similarity of gene expression data estimated with SAGE and Affymetrix GeneChips. BMC Genomics 6: 91.
-
(2005)
BMC Genomics
, vol.6
, pp. 91
-
-
van Ruissen, F.1
Ruijter, J.M.2
Schaaf, G.J.3
Asgharnegad, L.4
Zwijnenburg, D.A.5
-
25
-
-
2442679088
-
SAGE is far more sensitive than EST for detecting low-abundance transcripts
-
Sun M, Zhou G, Lee S, Chen J, Shi RZ, et al. (2004) SAGE is far more sensitive than EST for detecting low-abundance transcripts. BMC Genomics 5: 1-4.
-
(2004)
BMC Genomics
, vol.5
, pp. 1-4
-
-
Sun, M.1
Zhou, G.2
Lee, S.3
Chen, J.4
Shi, R.Z.5
-
26
-
-
34047118063
-
Identification of novel universal housekeeping genes by statistical analysis of microarray data
-
Lee S, Jo M, Lee J, Koh SS, Kim S (2007) Identification of novel universal housekeeping genes by statistical analysis of microarray data. J Biochem Mol Biol 40: 226-231.
-
(2007)
J Biochem Mol Biol
, vol.40
, pp. 226-231
-
-
Lee, S.1
Jo, M.2
Lee, J.3
Koh, S.S.4
Kim, S.5
-
27
-
-
0038692119
-
Human housekeeping genes are compact
-
Eisenberg E, Levanon EY (2003) Human housekeeping genes are compact. Trends Genet 19: 362-365.
-
(2003)
Trends Genet
, vol.19
, pp. 362-365
-
-
Eisenberg, E.1
Levanon, E.Y.2
-
28
-
-
9144257282
-
The FunCat, a functional annotation scheme for systematic classification of proteins from whole genomes
-
Ruepp A, Zollner A, Maier D, Albermann K, Hani J, et al. (2004) The FunCat, a functional annotation scheme for systematic classification of proteins from whole genomes. Nucleic Acids Res 32: 5539-5545.
-
(2004)
Nucleic Acids Res
, vol.32
, pp. 5539-5545
-
-
Ruepp, A.1
Zollner, A.2
Maier, D.3
Albermann, K.4
Hani, J.5
-
29
-
-
2442684104
-
An evaluation of new criteria for CpG islands in the human genome as gene markers
-
Wang Y, Leung FC (2004) An evaluation of new criteria for CpG islands in the human genome as gene markers. Bioinformatics 20: 1170-1177.
-
(2004)
Bioinformatics
, vol.20
, pp. 1170-1177
-
-
Wang, Y.1
Leung, F.C.2
-
30
-
-
0037435395
-
Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data
-
Ramakers C, Ruijter JM, Deprez RH, Moorman AF (2003) Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data. Neurosci Lett 339: 62-66.
-
(2003)
Neurosci Lett
, vol.339
, pp. 62-66
-
-
Ramakers, C.1
Ruijter, J.M.2
Deprez, R.H.3
Moorman, A.F.4
-
31
-
-
3442876969
-
Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets
-
Andersen CL, Jensen JL, Orntoft TF (2004) Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64: 5245-5250.
-
(2004)
Cancer Res
, vol.64
, pp. 5245-5250
-
-
Andersen, C.L.1
Jensen, J.L.2
Orntoft, T.F.3
-
32
-
-
0035931072
-
A compendium of gene expression in normal human tissues
-
Hsiao LL, Dangond F, Yoshida T, Hong R, Jensen RV, et al. (2001) A compendium of gene expression in normal human tissues. Physiol Genomics 7: 97-104.
-
(2001)
Physiol Genomics
, vol.7
, pp. 97-104
-
-
Hsiao, L.L.1
Dangond, F.2
Yoshida, T.3
Hong, R.4
Jensen, R.V.5
-
33
-
-
0026742792
-
CpG islands as gene markers in the human genome
-
Larsen F, Gundersen G, Lopez R, Prydz H (1992) CpG islands as gene markers in the human genome. Genomics 13: 1095-1107.
-
(1992)
Genomics
, vol.13
, pp. 1095-1107
-
-
Larsen, F.1
Gundersen, G.2
Lopez, R.3
Prydz, H.4
-
34
-
-
0035183199
-
Determinants of CpG islands: Expression in early embryo and isochore structure
-
Ponger L, Duret L, Mouchiroud D (2001) Determinants of CpG islands: expression in early embryo and isochore structure. Genome Res 11: 1854-1860.
-
(2001)
Genome Res
, vol.11
, pp. 1854-1860
-
-
Ponger, L.1
Duret, L.2
Mouchiroud, D.3
-
35
-
-
0141995484
-
Selection of candidate housekeeping controls in tomato plants using EST data
-
passim
-
Coker JS, Davies E (2003) Selection of candidate housekeeping controls in tomato plants using EST data. Biotechniques 35: 740-742, 744, 746 passim.
-
(2003)
Biotechniques
, vol.35
-
-
Coker, J.S.1
Davies, E.2
-
36
-
-
0034720002
-
Comparison of human adult and fetal expression and identification of 535 housekeeping/ maintenance genes
-
Warrington JA, Nair A, Mahadevappa M, Tsyganskaya M (2000) Comparison of human adult and fetal expression and identification of 535 housekeeping/ maintenance genes. Physiol Genomics 2: 143-147.
-
(2000)
Physiol Genomics
, vol.2
, pp. 143-147
-
-
Warrington, J.A.1
Nair, A.2
Mahadevappa, M.3
Tsyganskaya, M.4
-
37
-
-
7244245443
-
Quantitative gene expression profiling in formalin-fixed, paraffin-embedded tissues using universal bead arrays
-
Bibikova M, Talantov D, Chudin E, Yeakley JM, Chen J, et al. (2004) Quantitative gene expression profiling in formalin-fixed, paraffin-embedded tissues using universal bead arrays. Am J Pathol 165: 1799-1807.
-
(2004)
Am J Pathol
, vol.165
, pp. 1799-1807
-
-
Bibikova, M.1
Talantov, D.2
Chudin, E.3
Yeakley, J.M.4
Chen, J.5
-
39
-
-
36448987120
-
-
Glenn ST, Jones CA, Liang P, Kaushik D, Gross KW, et al. (2007) Expression profiling of archival renal tumors by quantitative PCR to validate prognostic markers. Biotechniques 43: 639-640, 642-633, 647.
-
Glenn ST, Jones CA, Liang P, Kaushik D, Gross KW, et al. (2007) Expression profiling of archival renal tumors by quantitative PCR to validate prognostic markers. Biotechniques 43: 639-640, 642-633, 647.
-
-
-
-
40
-
-
19944422061
-
A multigene assay to predict recurrence of tamoxifen-treated, node-negative breast cancer
-
Paik S, Shak S, Tang G, Kim C, Baker J, et al. (2004) A multigene assay to predict recurrence of tamoxifen-treated, node-negative breast cancer. N Engl J Med 351: 2817-2826.
-
(2004)
N Engl J Med
, vol.351
, pp. 2817-2826
-
-
Paik, S.1
Shak, S.2
Tang, G.3
Kim, C.4
Baker, J.5
-
41
-
-
15444373286
-
Statistical modeling for selecting housekeeper genes
-
Szabo A, Perou CM, Karaca M, Perreard L, Quackenbush JF, et al. (2004) Statistical modeling for selecting housekeeper genes. Genome Biol 5: R59.
-
(2004)
Genome Biol
, vol.5
-
-
Szabo, A.1
Perou, C.M.2
Karaca, M.3
Perreard, L.4
Quackenbush, J.F.5
-
42
-
-
67650276777
-
-
Roche Applied Science (2005) Technical Note No. LC 15/2005.
-
Roche Applied Science (2005) Technical Note No. LC 15/2005.
-
-
-
-
43
-
-
0033872068
-
Detection of gene amplification in archival breast cancer specimens by laser-assisted microdissection and quantitative real-time polymerase chain reaction
-
Lehmann U, Glockner S, Kleeberger W, von Wasielewski HF, Kreipe H (2000) Detection of gene amplification in archival breast cancer specimens by laser-assisted microdissection and quantitative real-time polymerase chain reaction. Am J Pathol 156: 1855-1864.
-
(2000)
Am J Pathol
, vol.156
, pp. 1855-1864
-
-
Lehmann, U.1
Glockner, S.2
Kleeberger, W.3
von Wasielewski, H.F.4
Kreipe, H.5
|