Housekeeping gene variability in normal and cancerous colorectal, pancreatic, esophageal, gastric and hepatic tissues

Molecular and Cellular Probes

Volumn 19, Issue 2, 2005, Pages 101-109

  Rubie, Claudia ^a   Kempf, Katja ^a   Hans, Joachim ^a   Su, Tiefen ^a   Tilton, Bettina ^a   Georg, Thomas ^a   Brittner, Brigitte ^a   Ludwig, Bianca ^a   Schilling, Martin ^a  

^a SAARLAND UNIVERSITY   (Germany)

Author keywords

Cancer biopsies; Gene expression analysis; mRNA quantification; Normalization; Quantitative real time PCR; Reference genes

Indexed keywords

MESSENGER RNA;

ARTICLE; CANCER TISSUE; COLORECTAL CANCER; CONTROLLED STUDY; ESOPHAGUS CANCER; GENE EXPRESSION; GENE IDENTIFICATION; GENETIC VARIABILITY; HOUSEKEEPING GENE; HUMAN; HUMAN TISSUE; LIVER CANCER; LIVER METASTASIS; NUCLEOTIDE SEQUENCE; PANCREAS CANCER; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; STOMACH CANCER;

COLORECTAL NEOPLASMS; ESOPHAGEAL NEOPLASMS; ESOPHAGUS; GASTROINTESTINAL NEOPLASMS; GASTROINTESTINAL TRACT; GENE EXPRESSION PROFILING; HUMANS; LIVER; LIVER NEOPLASMS; PANCREAS; PANCREATIC NEOPLASMS; REFERENCE STANDARDS; RNA, MESSENGER; STOMACH; STOMACH NEOPLASMS;

EID: 12944307847     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcp.2004.10.001     Document Type: Article

References (35)

1. S.A. Bustin Quantification of mRNA using real-tune reverse transcription PCR (RT-PCR): trends and Problems J Mol Endocrinol 29 2002 23 39
2. -deltaCt method Methods 25 2001 402 408
3. W. Pfaffl A new mathematical model for relative quantification in real-time RT-PCR Nucl Acids Res 29 2001 2002 2007
4. Y. Wu, and J.L. Rees Variation in epidermal housekeeping gene expression in different pathological states Acta Derm Venerol 80 2000 2 3
5. C. Tricarico, P. Pinzani, S. Bianchi, M. Paglierani, V. Distante, and M. Pazzagli Quantitative real-tine reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies Anal Biochem 309 2002 293 300
6. E. Beillard, N. Pallisgaard, V.M. Van der Velden, W Bi, R Dee, and E Van der Schoot Evaluation of candidate control genes for diagnosis and residual disease detection in leukemic patients using real-time quantitative reverse-transcriptase polymerase chain reaction (RQ-PCR) - a Europe against cancer program Leukemia 17 2003 2474 2486
7. O. Thellin, W. Zorzi, and B. Lakaye Housekeeping genes as internal standards: use and limits J Biotechnol 75 1999 291 295
8. D.L. Foss, M.J. Baarsch, and M.P. Murtaugh Regulation of hypoxanthine phosphoribosyltransferase, glyceraldehydes 3 phosphate dehydrogenases and beta-actin mRNA expression in porcine immune cells and tissues Anim Biotechnol 9 1998 67 78
9. T. Suzuki, P.J. Higgins, and D.R. Crawford Control selection for RNA quantitation Biotechniques 29 2000 332 337
10. T.J. Chang, C.C. Juan, P.H. Yin, C.W. Chi, and H.J. Tsay Up-regulation of beta-actin, cyclophilin and GAPDH in ns1s1 rat hepatoma Oncol Rep 5 1998 469 471
11. N.W. Marten, E.J. Burke, J.M. Hayden, and D.S. Strass Effect of amino acid limitation on the expression of 19 genes in rat hepatoma cells FASEB J 8 2000 538 544
12. J. Lupberger, K.A. Kreuzer, G. Baskayanak, U.R. Peters, P. le Coutre, and C.A. Schmidt Quantitative analysis of beta-actin, beta-2-n1icroglobulin and porphobilinogen deaminase mRNA and their comparison as control transcripts for RT-PCR Mol Cell Probes 16 2002 25 30
13. C. Blanquicett, M.R. Johnson, M. Heslin, and R.B. Diasio Housekeeping gene variability in normal and carcinomatous colorectal and liver tissues: applications in pharmacogenomic gene expression studies Anal Biochem 303 2002 209 214
14. H. Zhong, and J. Simons Direct comparison of GAPDH, β-actin, cyclophilin and 28SrRNA as internal standards for quantifying RNA levels under hypoxia Biochem Biophys Res Commun 259 1999 523 526
15. J.L. Aerts, M.I. Gonzales, and S.L. Topalian Selection of approriate control genes to assess expression of tumor antigens using real-time RT-PCR Biotechniques 36 2004 84 86
16. A. Oikarinen, J. Makela, T. Vuorio, and E. Vuorio Comparison on collagen gene expression in the developing chick embryo tendon and heart. Tissue and development time-dependent action of dexamethasone Biochim Biophys Acta 1089 1991 40 46
17. K. Tokunaga, Y. Nakamura, K. Sakata, K. Fujimori, M. Ohkubo, and K. Sawada Enhanced expression of a glyceraldehydes-3-phosphate dehydrogenase gene in human lung cancers Cancer Res 47 1987 5616 5619
18. S.A. Bustin Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays J Mol Endocrinol 25 2000 169 193
19. T.D. Schmittgen, and B.A. Zakrajsek Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR J Biochem Biophys Meth 46 2000 69 81
20. E.M. Glare, M. Divjak, M.J. Bailey, and E.H. Walters Beta-actin and GAPDH housekeeping gene expression in asthmatic airways is variable and not suitable for normalising mRNA levels Thorax 9 2002 765 770
21. I. Winer, C.K. Jung, I. Shackel, and P.M. Williams Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro Anal Biochem 270 1999 41 49
22. S.J. Wall, and D.R. Edwards Quantitative reverse transcription-polymerase chain reaction (RT-PCR): a comparison of primer-dropping, competitive, and real-time RT-PCRs Anal Biochem 300 2002 269 273
23. W.G. Tatton, R.M. Chalmers-Redman, M.W. Elstner, W. Leesch, F.B. Jagodzinski, and D.P. Stupak Glyceraldehyde-3-phosphate dehydrogenase in neurodegeneration and apoptosis signaling J Neural Transm Suppl 60 2000 77 100
24. D. Goidin, A. Mammessier, M.J. Staquet, D. Schmitt, and O. Berthier-Vergnes Ribosomal 18S RNA prevails over glyceraldehyde-3-phosphate dehydrogenase and beta-actin genes as internal standard for quantitative comparison of mRNA levels in invasive and noninvasive human melanoma cell subpopulations Anal Biochem 295 2001 17 21
25. M.C. Finnegan, J.R. Goepel, B.W. Hancock, and M.H. Goyns Investigation of the expression of housekeeping genes in non-Hodgkin's lymphoma Leuk Lymphoma 10 1993 387 393
26. M. Solanas, R. Moral, and E. Escrich Unsuitability of using ribosomal RNA as loading control for Northern blot analyses related to the imbalance between messenger and ribosomal RNA content in rat mammary tumors Anal Biochem 288 2001 99 102
27. A. Radonic, S. Thulke, I.M. Mackay, O. Landt, W. Siegert, and A. Nitsche Guideline to reference gene selection for quantitative real-time PCR Biochem Biophys Res Commun 313 2004 856 862
28. W.C. Shen, and M.R. Green Yeast TAF(II)145 functions as a core promoter selectivity factor, not as a general coactivator Cell 90 1997 615 624
29. B. Li, and J.C. Reese Derepression of DNA damage-regulated genes requires yeast TAF(II)s EMBO J 19 2000 4091 4100
30. J. Acker, O. Muroni, M.G. Mattei, C. Kedinger, and M. Vigneron The gene (POLR2L) encoding the hRPB7.6 subunit of human RNA polymerase Genomics 32 1996 86 90
31. J. Reichrath, C. Welter, T. Mitschele, U. Classen, V. Meineke, and W. Tilgen Different expression patterns of calpain isozymes 1 and 2 (CAPN1 and 2) in squamous cell carcinomas (SCC) and basal cell carcinomas (BCC) of human skin J Pathol 199 2003 509 516
32. J.H. Lee, C.W. Lee, S.G. Lee, M.S. Yang, M.K. Hong, and J.J. Kim Comparison of polymerase chain reaction with adenosine deaminase activity in pericardial fluid for the diagnosis of tuberculous pericarditis Am J Med 113 2002 519 521
33. M. Gulec, H. Akin, H. Yuce, E. Ergin, H. Elyas, and O. Yalcin Adenosine deaminase and xanthine oxidase activities in bladder washing fluid from patients with bladder cancer: a preliminary study Clin Biochem 36 2003 193 196
34. H.S. Ozturk, M. Karaayvaz, M. Kacmaz, M. Kavutcu, H. Akgul, and I. Durak Activities of the enzymes participating in purine and free-radical metabolism in cancerous human colorectal tissues Cancer Biochem Biophys l6 1998 l57 168
35. G.P. Conolly Hypoxanthine-guanine phosphoribosyltransferase-deficiency produces aberrant neurite outgrowth of rodent neuroblastoma used to model the neurological disorder Lesch Nyhan syndrome Neurosci Lett 314 2001 61 64