β-Actin - An unsuitable internal control for RT-PCR

Molecular and Cellular Probes

Volumn 15, Issue 5, 2001, Pages 307-311

  Selvey, S ^a   Thompson, E W ^a   Matthaei, K ^a   Lea, R A ^a   Irving, M G ^a   Griffiths, L R ^a  

^a GRIFFITH UNIVERSITY   (Australia)

Author keywords

Housekeeping gene; Quantitation; RT PCR; actin

Indexed keywords

BETA ACTIN; INTERSTITIAL COLLAGENASE; MATRIGEL;

ACCURACY; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DOSE RESPONSE; GENE CONTROL; GENE EXPRESSION; GENE TARGETING; MOUSE; NONHUMAN; PRIORITY JOURNAL; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPECTROPHOTOMETRY;

EID: 0035197018     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1006/mcpr.2001.0376     Document Type: Article

References (12)

1. Message amplification phenotyping (MAPPing)-principles, practice and potential
2. Construction of an internal control to quantitate multiple porcine cytokine mRNAs by RT-PCR
3. Abundance of leptin mRNA in fetal adipose tissue is related to fetal body weight
4. Effects of fasting on the expression of gastrin and various housekeeping genes in the pancreas and upper digestive tract of rats
5. Semi-quantitative RT-PCR for comparison of mRNAs in cells with different amounts of housekeeping gene transcripts
6. Rapid construction of deleted DNA fragments for use as internal standards in competitive PCR
7. Nucleotide sequence of a full-length cDNA for mouse cytoskeletal beta-actin mRNA
8. Membrane-type matrix metalloproteinase (MT-MMP) gene is expressed in stromal cells of human colon, breast, and head and neck carcinomas
9. Changes in beta-actin mRNA expression in remodeling canine myocardium
10. Regulation of hypoxanthine phosphoribosyltransferase, glyceraldehyde-3-phosphate dehydrogenase and beta-actin mRNA expression in porcine immune cells and tissues
11. Direct comparison of GAPDH, beta-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia
12. Assessment of template quality by the incorporation of an internal control into a RT-PCR for the detection of rabies and rabies-related viruses