Ribosomal 18S RNA prevails over glyceraldehyde-3-phosphate dehydrogenase and β-actin genes as internal standard for quantitative comparison of mRNA levels in invasive and noninvasive human melanoma cell subpopulations

Analytical Biochemistry

Volumn 295, Issue 1, 2001, Pages 17-21

  Goidin, Didier ^a   Mamessier, Audrey ^a   Staquet, Marie Jeanne ^a   Schmitt, Daniel ^a   Berthier Vergnes, Odile ^a  

^a EDOUARD HERRIOT HOSPITAL   (France)

Author keywords

cDNA array; Duplex relative RT PCR; GAPDH; Invasive and metastatic potential; Melanoma cells; Ribosomal 18S RNA; actin

Indexed keywords

CYTOLOGY; DERMATOLOGY; GENE EXPRESSION; ONCOLOGY; POLYMERASE CHAIN REACTION; RNA;

CDNA ARRAYS; DUPLEX RELATIVE RT-PCR; GAPDH; MELANOMA CELLS; METASTATIC POTENTIAL;

PROTEINS;

BETA ACTIN;

ARTICLE; CANCER INVASION; CELL SUBPOPULATION; GENE EXPRESSION REGULATION; HOUSEKEEPING GENE; HUMAN; HUMAN CELL; MALIGNANT TRANSFORMATION; MELANOMA CELL; MESSENGER RNA SYNTHESIS; METASTASIS POTENTIAL; MOLECULAR CLONING; PRIORITY JOURNAL; QUANTITATIVE ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RIBOSOME SUBUNIT; STATISTICAL ANALYSIS;

EID: 0035423813     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5171     Document Type: Article

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