Engineering algae for biohydrogen and biofuel production

Current Opinion in Biotechnology

Volumn 20, Issue 3, 2009, Pages 264-271

  Beer, Laura L ^a   Boyd, Eric S ^b   Peters, John W ^b   Posewitz, Matthew C ^a  

^a NSF Engineering Research Center for Reinventing the Nation s Urban Water Infrastructure   (United States)

^b MONTANA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIO-ENERGY; BIO-HYDROGEN; BIOFUEL PRODUCTION; ENDOGENOUS GENES; EUKARYOTIC ALGAE; GENETIC TECHNIQUES; MICRO-ALGAE; PHOTOSYNTHETIC EFFICIENCY; SALT WATER; TRANSGENE EXPRESSION;

BIOTECHNOLOGY; DIESEL FUELS; FUEL CELLS; GENES; PHYTOPLANKTON; SOLAR ENERGY;

BIOFUELS;

BIOFUEL; BIOHYDROGEN; CARBON; DIESEL FUEL; HYDROGEN; LIPID; STARCH; UNCLASSIFIED DRUG;

ALGA; ALGAL GENETICS; BIOFUEL PRODUCTION; BIOTECHNOLOGICAL PRODUCTION; CARBON STORAGE; CHLAMYDOMONAS REINHARDTII; ENERGY CONVERSION; GENE DISRUPTION; GENE EXPRESSION; GENETIC ENGINEERING; GENETIC MANIPULATION; LIPOGENESIS; METABOLIC ENGINEERING; MICROALGA; NONHUMAN; PHOTOSYNTHESIS; PLANT METABOLISM; PRIORITY JOURNAL; PROCESS OPTIMIZATION; RENEWABLE ENERGY; REVIEW; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; SIGNAL TRANSDUCTION; SOLAR ENERGY; TRANSGENE;

ALGAE; BIOELECTRIC ENERGY SOURCES; BIOTECHNOLOGY; CONSERVATION OF ENERGY RESOURCES;

ALGAE; EUKARYOTA;

EID: 67649757166     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.copbio.2009.06.002     Document Type: Review

References (51)

1. 2 production from marine and freshwater species of green algae during sulfur deprivation and considerations for bioreactor design. Int J Hyd Energy 33 (2008) 511-521
2. Dismukes G.C., Carrieri D., Bennette N., Ananyev G.M., and Posewitz M.C. Aquatic phototrophs: efficient alternatives to land-based crops for biofuels. Curr Opin Biotechnol 19 (2008) 235-240
3. Hu Q., Sommerfeld M., Jarvis E., Ghirardi M., Posewitz M., Seibert M., and Darzins A. Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J 54 (2008) 621-639
4. Posewitz M.C., Dubini A., Meuser J.E., Seibert M., and Ghirardi M.L. Hydrogenases, hydrogen production and anoxia. In: Stern D.B. (Ed). Organellar and Metabolic Processes. edn 2. In: Harris E.E.. (Ed). The Chlamydomonas Sourcebook Vol. 2 (2009), Academic Press 217-246
5. Schenk P., Thomas-Hall S., Stephens E., Marx U., Mussgnug J., Posten C., Kruse O., and Hankamer B. Second generation biofuels: high-efficiency microalgae for biodiesel production. Bioenerg Res 1 (2008) 20-43. The review article provides an overview of second-generation biodiesel production systems using microalgae. Sustainability, photosynthetic conversion efficiencies, and the requirements, advantages and disadvantages of microalgal feedstocks are discussed.
6. Grossman A.R. Acclimation of Chlamydomonas reinhardtii to its nutrient environment. Protist 151 (2000) 201-224
7. Naumann B., Busch A., Allmer J., Ostendorf E., Zeller M., Kirchhoff H., and Hippler M. Comparative quantitative proteomics to investigate the remodeling of bioenergetic pathways under iron deficiency in Chlamydomonas reinhardtii. Proteomics 7 (2007) 3964-3979
8. Nguyen A.V., Thomas-Hall S.R., Malnoe A., Timmins M., Mussgnug J.H., Rupprecht J., Kruse O., Hankamer B., and Schenk P.M. Transcriptome for photobiological hydrogen production induced by sulfur deprivation in the green alga Chlamydomonas reinhardtii. Eukaryot Cell 7 (2008) 1965-1979
9. Mus F., Dubini A., Seibert M., Posewitz M.C., and Grossman A.R. Anaerobic acclimation in Chlamydomonas reinhardtii: anoxic gene expression, hydrogenase induction and metabolic pathways. J Biol Chem 282 (2007) 25475-25486
10. Dubini A., Mus F., Seibert M., Grossman A.R., and Posewitz M.C. Flexibility in anaerobic metabolism as revealed in a mutant of Chlamydomonas reinhardtii lacking hydrogenase activity. J Biol Chem 284 (2009) 7201-7213
11. Walker T.L., Collet C., and Purton S. Algal transgenics in the genomic era. J Phycol 41 (2005) 1077-1093
12. Neupert J., Karcher D., and Bock R. Generation of Chlamydomonas strains that efficiently express nuclear transgenes. Plant J 57 (2008) 1140-1150
13. Croft M.T., Moulin M., Webb M.E., and Smith A.G. Thiamine biosynthesis in algae is regulated by riboswitches. Proc Natl Acad Sci U S A 104 (2007) 20770-20775
14. Shao N., and Bock R. A codon-optimized luciferase from Gaussia princeps facilitates the in vivo monitoring of gene expression in the model alga Chlamydomonas reinhardtii. Curr Genet 53 (2008) 381-388
15. Surzycki R., Cournac L., Peltier G., and Rochaix J.D. Potential for hydrogen production with inducible chloroplast gene expression in Chlamydomonas. Proc Natl Acad Sci U S A 104 (2007) 17548-17553
16. Zorin B., Lu Y., Sizova I., and Hegemann P. Nuclear gene targeting in Chlamydomonas as exemplified by disruption of the PHOT gene. Gene 432 (2009) 91-96. The authors report the continued improvement of homologous recombination techniques in the green alga Chlamydomonas reinhardtii.
17. Molnar A., Bassett A., Thuenemann E., Schwach F., Karkare S., Ossowski S., Weigel D., and Baulcombe D. Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii. Plant J 58 (2009) 165-174. This article describes C. reinhardtii miRNAs that direct site-specific cleavage of target mRNA with miRNA-complementary motifs and, presumably, act as regulatory molecules in growth and development. A novel artificial miRNA system based on ligation of DNA oligonucleotides that can be used for specific high-throughput gene silencing in green algae is described.
18. Zhao T., Wang W., Bai X., and Qi Y. Gene silencing by artificial microRNAs in Chlamydomonas. Plant J 58 (2009) 157-164. The researchers have developed an artificial miRNA-based strategy to silence gene expression in Chlamydomonas. The authors demonstrated that two amiRNAs could be expressed from a dimeric amiRNA precursor to knockdown the transcript levels of two unrelated genes simultaneously.
19. Rohr J., Sarkar N., Balenger S., Jeong B.R., and Cerutti H. Tandem inverted repeat system for selection of effective transgenic RNAi strains in Chlamydomonas. Plant J 40 (2004) 611-621
20. Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J., Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L., et al. The Chlamydomonas genome reveals the evolution of key animal and plant functions. Science 318 (2007) 245-250
21. Grossman A.R., Croft M., Gladyshev V.N., Merchant S.S., Posewitz M.C., Prochnik S., and Spalding M.H. Novel metabolism in Chlamydomonas through the lens of genomics. Curr Opin Plant Biol 10 (2007) 190-198
22. Margulies M., Egholm M., Altman W.E., Attiya S., Bader J.S., Bemben L.A., Berka J., Braverman M.S., Chen Y.-J., Chen Z., et al. Genome sequencing in microfabricated high-density picolitre reactors. Nature 437 (2005) 376-380
23. Zhao T., Li G.L., Mi S.J., Li S., Hannon G.J., Wang X.J., and Qi Y.J. A complex system of small RNAs in the unicellular green alga Chlamydomonas reinhardtii. Genes Dev 21 (2007) 1190-1203. This research showed that Chlamydomonas contains numerous microRNAs (miRNAs) that can regulate the transcript levels of endogenous genes.
24. Molnar A., Schwach F., Studholme D.J., Thuenemann E.C., and Baulcombe D.C. miRNAs control gene expression in the single-cell alga Chlamydomonas reinhardtii. Nature 447 (2007) 1126-1129. This research showed that Chlamydomonas contains many microRNAs (miRNAs) that can downregulate expression of endogenous genes.
25. Eamens A., Wang M.B., Smith N.A., and Waterhouse P.M. RNA silencing in plants: yesterday, today, and tomorrow. Plant Phys 147 (2008) 456-468
26. Baerenfaller K., Grossmann J., Grobei M.A., Hull R., Hirsch-Hoffmann M., Yalovsky S., Zimmermann P., Grossniklaus U., Gruissem W., and Baginsky S. Genome-scale proteomics reveals Arabidopsis thaliana gene models and proteome dynamics. Science 320 (2008) 938-941
27. 2-production phenotype compared to the parental strain. The mutant is blocked in cyclic e-transport, has a modified respiratory metabolism, and large starch reserves.
28. Mussgnug J.H., Thomas-Hall S., Rupprecht J., Foo A., Klassen V., McDowall A., Schenk P.M., Kruse O., and Hankamer B. Engineering photosynthetic light capture: impacts on improved solar energy to biomass conversion. Plant Biotechnol J 5 (2007) 802-814
29. Tanaka A., and Melis A. Irradiance-dependent changes in the size and composition of the chlorophyll a-b light-harvesting complex in the green alga Dunaliella salina. Plant Cell Physiol 38 (1997) 17-24
30. Tetali S.D., Mitra M., and Melis A. Development of the light-harvesting chlorophyll antenna in the green alga Chlamydomonas reinhardtii is regulated by the novel Tla1 gene. Planta 225 (2007) 813-829
31. 2-production capacity.
32. Posewitz M.C., Smolinski S.L., Kanakagiri S., Melis A., Seibert M., and Ghirardi M.L. Hydrogen photoproduction is attenuated by disruption of an isoamylase gene in Chlamydomonas reinhardtii. Plant Cell 16 (2004) 2151-2163
33. Ball S.G. The intricate pathway of starch biosynthesis and degradation in the monocellular alga Chlamydomonas reinhardtii. Aust J Chem 55 (2002) 49-59
34. Moellering E.R., Ouyang Y., Mamedov T.G., and Chollet R. The two divergent PEP-carboxylase catalytic subunits in the green microalga Chlamydomonas reinhardtii respond reversibly to inorganic-N supply and co-exist in the high-molecular-mass, hetero-oligomeric Class-2 PEPC complex. FEBS Lett 581 (2007) 4871-4876
35. Riekhof W.R., and Benning C. Glycerolipid biosynthesis. In: Stern D.B. (Ed). The Chlamydomonas Sourcebook Organellar and Metabolic Processes. edn 2. In: Harris E.E.. (Ed). The Chlamydomonas Sourcebook Vol. 2 (2009), Academic Press 41-68
36. Riekhof W.R., Sears B.B., and Benning C. Annotation of genes involved in glycerolipid biosynthesis in Chlamydomonas reinhardtii: discovery of the betaine lipid synthase BTA1(Cr). Eukaryot Cell 4 (2005) 242-252
37. Moseley J.L., Gonzalez-Ballester D., Pootakham W., Bailey S., and Grossman A.R. Genetic interactions between regulators of Chlamydomonas phosphorus and sulfur deprivation responses. Genetics 181 (2008) 889-905
38. Sugimoto K., Midorikawa T., Tsuzuki M., and Sato N. Upregulation of PG synthesis on sulfur-starvation for PS I in Chlamydomonas. Biochem Biophys Res Commun 369 (2008) 660-665
39. Sugimoto K., Sato N., and Tsuzuki M. Utilization of a chloroplast membrane sulfolipid as a major internal sulfur source for protein synthesis in the early phase of sulfur starvation in Chlamydomonas reinhardtii. FEBS Lett 581 (2007) 4519-4522
40. Nagy L.E., Meuser J.E., Plummer S., Seibert M., Ghirardi M.L., King P.W., Ahmann D., and Posewitz M.C. Application of gene-shuffling for the rapid generation of novel [FeFe]-hydrogenase libraries. Biotechnol Lett 29 (2007) 421-430
41. Boyd ES, Spear JR, Peters JW: [FeFe]-hydrogenase genetic diversity provides insight into molecular adaptation in a saline microbial mat community. Appl Environ Microbiol 2009, in press. This report details the development and application of robust primers for the highly specific amplification of novel [FeFe]-hydrogenases from environmental genomic DNA samples isolated from a saline system with high microbial diversity.
42. Meyer J. [FeFe] hydrogenases and their evolution: a genomic perspective. Cell Mol Life Sci 64 (2007) 1063-1084
43. Liu Y.G., and Whittier R.F. Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics 25 (1995) 674-681
44. Girbal L., von Abendroth G., Winkler M., Benton P.M.C., Meynial-Salles I., Croux C., Peters J.W., Happe T., and Soucaille P. Homologous and heterologous overexpression in Clostridium acetobutylicum and characterization of purified clostridial and algal Fe-only hydrogenases with high specific activities. Appl Environ Microbiol 71 (2005) 2777-2781
45. Posewitz M.C., King P.W., Smolinski S.L., Zhang L., Seibert M., and Ghirardi M.L. Discovery of two novel radical S-adenosylmethionine proteins required for the assembly of an active [Fe] hydrogenase. J Biol Chem 279 (2004) 25711-25720. The authors report the initial identification of the [FeFe]-hydrogenase maturases, and demonstrate the first heterologous expression of an [FeFe]-hydrogenase in a host lacking an endogenous [FeFe]-hydrogenase.
46. McGlynn S.E., Shepard E.M., Winslow M.A., Naumov A.V., Duschene K.S., Posewitz M.C., Broderick W.E., Broderick J.B., and Peters J.W. HydF as a scaffold protein in [FeFe] hydrogenase H-cluster biosynthesis. FEBS Lett 582 (2008) 2183-2187
47. Schubert T., Lenz O., Krause E., Volkmer R., and Friedrich B. Chaperones specific for the membrane-bound [NiFe]-hydrogenase interact with the Tat signal peptide of the small subunit precursor in Ralstonia eutropha H16. Mol Microbiol 66 (2007) 453-467
48. Ludwig M., Schubert T., Zebger I., Wisitruangsakul N., Saggu M., Strack A., Lenz O., Hildebrandt P., and Friedrich B. Concerted action of two novel auxiliary proteins in assembly of the active site in a membrane-bound [NiFe] hydrogenase. J Biol Chem 284 (2009) 2159-2168
49. Sybirna K., Antoine T., Lindberg P., Fourmond V., Rousset M., Mejean V., and Bottin H. Shewanella oneidensis: a new and efficient system for expression and maturation of heterologous [Fe-Fe] hydrogenase from Chlamydomonas reinhardtii. BMC Biotechnol 8 (2008) 73
50. Lenz O., Gleiche A., Strack A., and Friedrich B. Requirements for heterologous production of a complex metalloenzyme: the membrane-bound [NiFe] hydrogenase. J Bacteriol 187 (2005) 6590-6595
51. King P.W., Posewitz M.C., Ghirardi M.L., and Seibert M. Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system. J Bacteriol 188 (2006) 2163-2172